LOCUS       BBF10K_003442           3017 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003442
VERSION     BBF10K_003442.1  
FEATURES             Location/Qualifiers
     CDS             5..897
                     /label=CD_PBCV-1_ligase
     CDS             complement(1940..2799)
                     /label=AmpR
     promoter        complement(2801..2904)
                     /label=AmpR promoter
     primer_bind     2923..2939
                     /label=M13 fwd
     primer_bind     complement(1078..1093)
                     /label=M13 rev
     rep_origin      complement(1161..1748)
                     /label=ColE1
     primer_bind     995..1013
                     /label=SLiCE-for
     primer_bind     complement(2967..2984)
                     /label=SLiCE-rev
     misc_feature    1752..1941
                     /label=spacer
     CDS             1112..1162
                     /label=spacer
     terminator      1028..1075
                     /label=T7 terminator
     terminator      2940..2971
                     /label=tonB terminator
     misc_feature    990..2990
                     /label=pOpen_v3 backbone
     misc_feature    3011..3016
                     /label=BsaI_site
     misc_feature    complement(903..908)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=AATG
     misc_feature    898..901
                     /label=AGGT
ORIGIN
        1 AATGGCAATC ACAAAGCCAT TGCTCGCCGC CACACTTGAA AATATCGAGG ACGTGCAATT
       61 TCCGTGTTTG GCAACTCCTA AAATAGATGG CATCAGGAGC GTGAAACAAA CGCAAATGCT
      121 CTCAAGAACT TTCAAACCAA TTAGAAACTC TGTCATGAAC CGCCTGCTTA CCGAACTGCT
      181 GCCGGAGGGC AGTGATGGTG AAATTAGCAT CGAAGGTGCC ACCTTTCAGG ACACCACGAG
      241 TGCGGTTATG ACGGGCCATA AAATGTATAA TGCTAAGTTT TCGTATTATT GGTTTGATTA
      301 TGTGACGGAC GACCCCCTGA AGAAATATAT TGATCGCGTA GAGGATATGA AGAACTATAT
      361 CACCGTTCAT CCTCACATTC TGGAACACGC GCAAGTAAAA ATTATTCCTC TGATTCCAGT
      421 TGAAATCAAC AATATTACTG AACTATTACA ATATGAACGA GATGTTTTGT CTAAAGGGTT
      481 CGAAGGAGTG ATGATACGAA AACCGGACGG AAAATACAAG TTCGGTCGTT CTACCTTGAA
      541 AGAAGGTATT CTACTGAAGA TGAAACAGTT CAAAGATGCG GAGGCGACTA TCATCAGCAT
      601 GACGGCGCTG TTTAAAAACA CAAACACCAA AACGAAAGAT AATTTTGGGT ACAGTAAACG
      661 TTCCACCCAT AAGAGTGGCA AAGTAGAGGA GGACGTGATG GGGAGCATCG AAGTCGATTA
      721 CGACGGCGTT GTGTTCTCGA TCGGCACCGG CTTTGATGCT GATCAGCGGC GCGATTTTTG
      781 GCAGAACAAA GAATCATACA TTGGTAAAAT GGTGAAGTTC AAATACTTCG AAATGGGCTC
      841 CAAGGATTGC CCGCGCTTTC CGGTCTTTAT TGGTATTCGT CACGAGGAGG ACCGTGGAGG
      901 TCGAGACCAC TTGAAATCTT GTCGCACGGT GGGCGTTATG GAACAGTCAA TAGGGATAAC
      961 AGGGTAATAG GATCTATCTC CAGCACTCTC GCTGAGGTGT CAATCGTCGG AGCCGCTGAG
     1021 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT
     1081 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG
     1141 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     1201 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     1261 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     1321 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     1381 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     1441 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     1501 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     1561 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     1621 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     1681 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     1741 AGGATCTCAA GAAGGCCTAC TATTAGCAAC AACGATCCTT TGATCTTTTC TACGGGGTCT
     1801 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG
     1861 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT
     1921 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
     1981 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG
     2041 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT
     2101 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA
     2161 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG
     2221 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG
     2281 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC
     2341 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG
     2401 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG
     2461 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG
     2521 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT
     2581 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG
     2641 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA
     2701 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA
     2761 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT
     2821 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG
     2881 AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG
     2941 TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTTCAGGT GGAGTGGGAG AACGATGATG
     3001 CTCACTCTCG GGTCTCA
//