LOCUS       BBF10K_003443           3047 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003443
VERSION     BBF10K_003443.1  
FEATURES             Location/Qualifiers
     CDS             5..927
                     /label=CD_HRP
     CDS             complement(1970..2829)
                     /label=AmpR
     promoter        complement(2831..2934)
                     /label=AmpR promoter
     primer_bind     2953..2969
                     /label=M13 fwd
     primer_bind     complement(1108..1123)
                     /label=M13 rev
     rep_origin      complement(1191..1778)
                     /label=ColE1
     primer_bind     1025..1043
                     /label=SLiCE-for
     primer_bind     complement(2997..3014)
                     /label=SLiCE-rev
     misc_feature    1782..1971
                     /label=spacer
     CDS             1142..1192
                     /label=spacer
     terminator      1058..1105
                     /label=T7 terminator
     terminator      2970..3001
                     /label=tonB terminator
     misc_feature    1020..3020
                     /label=pOpen_v3 backbone
     misc_feature    3041..3046
                     /label=BsaI_site
     misc_feature    complement(933..938)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=AATG
     misc_feature    928..931
                     /label=AGGT
ORIGIN
        1 AATGCTAACG CCCACTTTCT ATGATAACTC ATGTCCAGAT GTGAGTAATA TTGTTCGCGA
       61 TACCATTGTC AACGAGCTCC GCTCTGATCC GCGTATTGCC GCCTCGATAT TGCGTTTGCA
      121 CTTTCATGAC TGCTTTGTCA ATGGTTGTGA TGCGAGCATC CTGTTGGACA GCACAACCAG
      181 TTTTCGTACG GAAAAAGACG CGTTTGGTAA CGCAAACTCC GCGAGAGGCT TTCCGGTGAT
      241 TGATAGGATG AAAGCGGCGG TGGAATCAGC ATGCCCGCGG ACCGTGAGCT GTGCCGATTT
      301 ACTGACTATT GCGGCACAAC AGTCGGTAAC GTTAGCGGGC GGACCGAGCT GGCGCGTTCC
      361 GTTGGGTCGC CGTGACTCAC TGCAGGCTTT TCTGGATCTG GCTAACGCCA ACCTGCCCGC
      421 ACCATTTTTT ACCCTCCCAC AGCTGAAGGA TTCTTTTCGG AACGTAGGCT TAAATCGTTC
      481 CTCTGATCTG GTAGCACTGT CCGGGGGCCA TACCTTCGGA AAAAATCAAT GTCGCTTTAT
      541 CATGGACCGT CTGTACAACT TTTCCAACAC CGGCTTACCG GACCCTACAC TGAATACCAC
      601 TTATCTGCAA ACGCTTCGTG GCCTTTGCCC TCTCAATGGT AACTTAAGTG CCCTGGTTGA
      661 TTTCGATCTT CGTACCCCTA CGATCTTCGA CAATAAATAT TACGTTAATC TTGAGGAACA
      721 GAAAGGCCTG ATTCAGTCGG ACCAGGAACT GTTCTCTAGC CCGGATGCTA CCGATACAAT
      781 CCCGCTGGTG CGAAGTTTCG CTGATTCGAC GCAGACGTTC TTCAACGCGT TTGTCGAAGC
      841 CATGGACCGC ATGGGGAATA TTACTCCGCT GACAGGGACC CAAGGTCAGA TCCGCTTGAA
      901 TTGCCGCGTG GTCAACAGCA ATAGCGGAGG TCGAGACCAG TAATGGCTCG GATCGTCTTG
      961 CTGTACAGAA GTTCGGTCTA TAGGGATAAC AGGGTAATAC TCGTGTTTGT TACGCACCAC
     1021 GCTGAGGTGT CAATCGTCGG AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT
     1081 AAACGGGTCT TGAGGGGTTT TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG
     1141 ATGACACACA TTAACAAATT TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG
     1201 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
     1261 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
     1321 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
     1381 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
     1441 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
     1501 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
     1561 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
     1621 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
     1681 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
     1741 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGGCCTAC TATTAGCAAC
     1801 AACGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA
     1861 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA
     1921 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC
     1981 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA
     2041 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA
     2101 ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC
     2161 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT
     2221 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC
     2281 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT
     2341 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC
     2401 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG
     2461 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT
     2521 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG
     2581 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA
     2641 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG
     2701 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG
     2761 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT
     2821 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC
     2881 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC
     2941 TGCACCAGTC AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT
     3001 TGGTTCAGGT GGAGTGGGAG AAGAATTACT GACCCCTCGG GGTCTCA
//