LOCUS       BBF10K_003444           2831 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003444
VERSION     BBF10K_003444.1  
FEATURES             Location/Qualifiers
     CDS             5..711
                     /label=CD_TEV_protease
     CDS             complement(1754..2613)
                     /label=AmpR
     promoter        complement(2615..2718)
                     /label=AmpR promoter
     primer_bind     2737..2753
                     /label=M13 fwd
     primer_bind     complement(892..907)
                     /label=M13 rev
     rep_origin      complement(975..1562)
                     /label=ColE1
     primer_bind     809..827
                     /label=SLiCE-for
     primer_bind     complement(2781..2798)
                     /label=SLiCE-rev
     misc_feature    1566..1755
                     /label=spacer
     CDS             926..976
                     /label=spacer
     terminator      842..889
                     /label=T7 terminator
     terminator      2754..2785
                     /label=tonB terminator
     misc_feature    804..2804
                     /label=pOpen_v3 backbone
     misc_feature    2825..2830
                     /label=BsaI_site
     misc_feature    complement(717..722)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=AATG
     misc_feature    712..715
                     /label=AGGT
ORIGIN
        1 AATGGAAAGC TTGTTTAAGG GGCCGCGTGA TTACAACCCG ATATCGAGCA CCATTTGTCA
       61 TTTGACGAAT GAATCTGATG GGCACACAAC ATCGTTGTAT GGTATTGGAT TTGGTCCCTT
      121 CATCATTACA AACAAGCACT TGTTTAGAAG AAATAATGGA ACACTGTTGG TCCAATCACT
      181 ACATGGTGTA TTCAAGGTCA AGAACACCAC GACTTTGCAA CAACACCTCA TTGATGGGAG
      241 GGACATGATA ATTATTCGCA TGCCTAAGGA TTTCCCACCA TTTCCTCAAA AGCTGAAGTT
      301 TAGAGAGCCA CAGAGGGAGG AACGCATTTG TCTTGTGACA ACCAACTTCC AAACTAAGAG
      361 CATGTCTAGC ATGGTGTCAG ACACCAGTTG CACATTCCCT TCATCTGATG GCATATTCTG
      421 GAAGCATTGG ATTCAAACCA AGGATGGGCA GTGTGGCAGT CCATTAGTAT CAACTAGAGA
      481 TGGGTTCATT GTTGGTATAC ACTCAGCATC GAACTTCACC AACACAAACA ATTATTTCAC
      541 AAGCGTGCCG AAAAACTTCA TGGAATTGTT GACAAATCAG GAGGCGCAGC AGTGGGTTAG
      601 TGGTTGGCGA TTAAATGCTG ATTCAGTATT GTGGGGGGGC CATAAAGTTT TCATGGTGAA
      661 ACCTGAGGAA CCTTTTCAGC CAGTTAAGGA AGCGACGCAA CTCATGAATG GAGGTCGAGA
      721 CCAGCTCGAT GCCATAGTTC CTTTTCAAGG GAATACGATT TGGTTAGGGA TAACAGGGTA
      781 ATCCTATTCT TCCCCAGTAC CAACGCTGAG GTGTCAATCG TCGGAGCCGC TGAGCAATAA
      841 CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCA TGGTCATAGC
      901 TGTTTCCTGA GAGCTTGGCA GGTGATGACA CACATTAACA AATTTCGTGA GGAGTCTCCA
      961 GAAGAATGCC ATTAATTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
     1021 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
     1081 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
     1141 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT
     1201 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
     1261 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
     1321 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
     1381 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT
     1441 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
     1501 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
     1561 TCAAGAAGGC CTACTATTAG CAACAACGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT
     1621 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC
     1681 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA
     1741 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA
     1801 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC
     1861 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT
     1921 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA
     1981 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT
     2041 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT
     2101 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG
     2161 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC
     2221 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC
     2281 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG
     2341 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA
     2401 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA
     2461 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT
     2521 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG
     2581 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA
     2641 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT
     2701 AAACAAATAG GGGTTCCGCG CACCTGCACC AGTCAGTAAA ACGACGGCCA GTAGTCAAAA
     2761 GCCTCCGACC GGAGGCTTTT GACTTGGTTC AGGTGGAGTG GGAGAAGAAT TACTGACCCC
     2821 TCGGGGTCTC A
//