LOCUS       BBF10K_003445           2783 bp    DNA     circular     17-Jun-2025
ACCESSION   BBF10K_003445
VERSION     BBF10K_003445.1  
FEATURES             Location/Qualifiers
     CDS             5..663
                     /label=CD_SUMO_protease
     CDS             complement(1706..2565)
                     /label=AmpR
     promoter        complement(2567..2670)
                     /label=AmpR promoter
     primer_bind     2689..2705
                     /label=M13 fwd
     primer_bind     complement(844..859)
                     /label=M13 rev
     rep_origin      complement(927..1514)
                     /label=ColE1
     primer_bind     761..779
                     /label=SLiCE-for
     primer_bind     complement(2733..2750)
                     /label=SLiCE-rev
     misc_feature    1518..1707
                     /label=spacer
     CDS             878..928
                     /label=spacer
     terminator      794..841
                     /label=T7 terminator
     terminator      2706..2737
                     /label=tonB terminator
     misc_feature    756..2756
                     /label=pOpen_v3 backbone
     misc_feature    751..756
                     /label=BsaI_site
     misc_feature    complement(669..674)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=AATG
     misc_feature    664..667
                     /label=AGGT
ORIGIN
        1 AATGCTGGTG CCTGAACTGA ATGAGAAAGA TGATGACCAG GTCCAGAAAG CACTCGCGTC
       61 ACGCGAAAAC ACCCAGCTTA TGAATCGTGA TAACATCGAG ATCACGGTAC GCGATTTCAA
      121 AACCTTGGCC CCACGCCGAT GGCTGAACGA TACTATAATT GAGTTCTTTA TGAAGTATAT
      181 CGAAAAGAGT ACCCCGAATA CCGTCGCATT TAATAGCTTC TTTTATACTA ATTTGTCCGA
      241 ACGTGGATAC CAGGGCGTGC GCCGTTGGAT GAAGCGGAAA AAAACACAAA TTGATAAATT
      301 AGACAAAATC TTTACGCCCA TTAATCTGAA CCAAAGCCAT TGGGCTCTGG GCATCATTGA
      361 TCTAAAGAAA AAAACGATTG GTTACGTTGA CAGTCTGTCT AACGGCCCGA ATGCAATGTC
      421 GTTTGCTATT CTCACCGATC TTCAGAAATA CGTTATGGAA GAATCGAAAC ATACCATAGG
      481 TGAAGATTTC GATTTAATCC ACCTTGACTG CCCACAACAG CCGAACGGGT ATGACTGCGG
      541 TATTTACGTG TGTATGAACA CACTGTATGG CAGCGCCGAT GCGCCGTTAG ACTTTGACTA
      601 TAAAGATGCC ATTCGTATGC GTCGCTTCAT TGCGCACCTG ATCTTGACGG ATGCGCTGAA
      661 AGGAGGTCGA GACCACAACC TGGTATGCAG TCCTTGTGGT CATCGGTTCC TTGGCGTAGG
      721 GATAACAGGG TAATATTAGA GGCTCAAAAG GGTCTCGCTG AGGTGTCAAT CGTCGGAGCC
      781 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG
      841 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT
      901 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
      961 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     1021 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     1081 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     1141 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     1201 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     1261 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     1321 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
     1381 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     1441 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     1501 AAAAAAAGGA TCTCAAGAAG GCCTACTATT AGCAACAACG ATCCTTTGAT CTTTTCTACG
     1561 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
     1621 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT
     1681 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA
     1741 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
     1801 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA
     1861 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
     1921 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT
     1981 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA
     2041 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
     2101 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
     2161 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
     2221 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA
     2281 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG
     2341 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
     2401 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
     2461 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
     2521 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT
     2581 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT
     2641 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC
     2701 CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT TCAGGTGGAG TGGGAGAAGA
     2761 ATTACTGACC CCTCGGGGTC TCA
//