LOCUS       BBF10K_003446           2570 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003446
VERSION     BBF10K_003446.1  
FEATURES             Location/Qualifiers
     CDS             5..450
                     /label=CD_Calmodulin
     CDS             complement(1493..2352)
                     /label=AmpR
     promoter        complement(2354..2457)
                     /label=AmpR promoter
     primer_bind     2476..2492
                     /label=M13 fwd
     primer_bind     complement(631..646)
                     /label=M13 rev
     rep_origin      complement(714..1301)
                     /label=ColE1
     primer_bind     548..566
                     /label=SLiCE-for
     primer_bind     complement(2520..2537)
                     /label=SLiCE-rev
     misc_feature    1305..1494
                     /label=spacer
     CDS             665..715
                     /label=spacer
     terminator      581..628
                     /label=T7 terminator
     terminator      2493..2524
                     /label=tonB terminator
     misc_feature    543..2543
                     /label=pOpen_v3 backbone
     misc_feature    2564..2569
                     /label=BsaI_site
     misc_feature    complement(456..461)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=AATG
     misc_feature    451..454
                     /label=AGGT
ORIGIN
        1 AATGGCGGAC CAGCTGACGG AGGAGCAAAT TGCGGAATTT AAAGAGGCTT TCTCGCTGTT
       61 CGACAAAGAC GGCGATGGTA CAATCACCAC TAAGGAACTG GGAACCGTGA TGCGGTCCCT
      121 TGGGCAGAAC CCGACTGAAG CCGAACTGCA AGATATGATA AATGAGGTGG ATGCAGATGG
      181 CAACGGCACC ATTGATTTCC CAGAATTTTT GACAATGATG GCACGTAAAA TGAAAGATAC
      241 CGATAGTGAG GAAGAAATTC GCGAAGCCTT TCGAGTATTT GATAAGGATG GTAATGGGTA
      301 CATTAGCGCT GCCGAACTCC GTCATGTCAT GACCAATTTA GGTGAAAAAC TGACGGATGA
      361 GGAAGTTGAC GAGATGATCC GCGAAGCGGA CATCGATGGC GACGGTCAGG TGAACTATGA
      421 AGAGTTTGTT CAGATGATGA CGGCGAAAGG AGGTCGAGAC CAGGCGGTGT CTACATATCC
      481 TTGGTTAAAC TAGAGCACAA TAATAGGGAT AACAGGGTAA TAGGACGTCA CTTCTGAGAG
      541 AGCGCTGAGG TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC
      601 TCTAAACGGG TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG AGCTTGGCAG
      661 GTGATGACAC ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA TTAATTTCCA
      721 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
      781 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
      841 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
      901 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
      961 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG
     1021 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
     1081 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
     1141 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
     1201 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
     1261 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGGCC TACTATTAGC
     1321 AACAACGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
     1381 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA
     1441 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA
     1501 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC
     1561 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT
     1621 GCAATGATAC CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
     1681 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT
     1741 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT
     1801 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC
     1861 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC
     1921 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT
     1981 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT
     2041 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC
     2101 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT
     2161 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG
     2221 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT
     2281 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA
     2341 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT
     2401 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC
     2461 ACCTGCACCA GTCAGTAAAA CGACGGCCAG TAGTCAAAAG CCTCCGACCG GAGGCTTTTG
     2521 ACTTGGTTCA GGTGGAGTGG GAGAAGAATT ACTGACCCCT CGGGGTCTCA
//