LOCUS       BBF10K_003447           2843 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003447
VERSION     BBF10K_003447.1  
FEATURES             Location/Qualifiers
     CDS             5..723
                     /label=CD_fuGFP
     CDS             complement(1766..2625)
                     /label=AmpR
     promoter        complement(2627..2730)
                     /label=AmpR promoter
     primer_bind     2749..2765
                     /label=M13 fwd
     primer_bind     complement(904..919)
                     /label=M13 rev
     rep_origin      complement(987..1574)
                     /label=ColE1
     primer_bind     821..839
                     /label=SLiCE-for
     primer_bind     complement(2793..2810)
                     /label=SLiCE-rev
     misc_feature    1578..1767
                     /label=spacer
     CDS             938..988
                     /label=spacer
     terminator      854..901
                     /label=T7 terminator
     terminator      2766..2797
                     /label=tonB terminator
     misc_feature    816..2816
                     /label=pOpen_v3 backbone
     misc_feature    2837..2842
                     /label=BsaI_site
     misc_feature    complement(729..734)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=AATG
     misc_feature    724..727
                     /label=AGGT
ORIGIN
        1 AATGGTTTCT AGCGGTGAAG ATATCTTCTC AGGCCTGGTC CCGATTCTGA TCGAGCTGGA
       61 GGGCGATGTG AATGGCCACC GCTTCTCTGT TCGTGGTGAA GGTTATGGCG ACGCCTCTAA
      121 CGGCAAACTG GAAATTAAGT TCATCTGCAC CACCGGTCGT CTGCCGGTTC CGTGGCCGAC
      181 GCTGGTGACT ACTCTGTCTT ACGGTGTCCA GTGCTTCGCA AAATACCCGG AACACATGCG
      241 TCAGAATGAT TTTTTCAAGT CCGCCATGCC AGATGGCTAT GTTCAGGAAC GTACCATCAG
      301 CTTCAAAGAG GACGGTACGT ACAAAACCCG TGCGGAAGTT AAATTTGAAG GTGAAGCGCT
      361 GGTGAACCGT ATCGACTTAA AAGGTCTGGA GTTCAAGGAA GATGGCAACA TTCTGGGCCA
      421 CAAACTGGAA TACAGCTTCA ACAGCCACTA TGTTTATATT ACCGCGGACA AAAATCGCAA
      481 CGGCCTGGAG GCGCAGTTCC GTATCCGTCA CAACGTCGAC GACGGTTCTG TCCAGCTTGC
      541 GGACCATTAC CAGCAGAACA CCCCGATCGG TGAGGGCCCA GTACTGCTCC CGGAGCAACA
      601 CTACCTGACG ACTAACTCTG TCCTGTCCAA AGACCCGCAG GAACGCCGTG ATCACATGGT
      661 TCTGGTTGAA TTTGTGACCG CTGCCGGCCT GTCGCTGGGC ATGGACGAAC TGTATAAATC
      721 CGGAGGTCGA GACCAGTTGT ATCCTTCTGG GCCTTCCGTA GAGCCCTGGG TCGTGTTAGG
      781 GATAACAGGG TAATCACAGC CGGGAGCCAT GACCCCGCTG AGGTGTCAAT CGTCGGAGCC
      841 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG
      901 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT
      961 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
     1021 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     1081 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     1141 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     1201 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     1261 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     1321 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     1381 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
     1441 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     1501 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     1561 AAAAAAAGGA TCTCAAGAAG GCCTACTATT AGCAACAACG ATCCTTTGAT CTTTTCTACG
     1621 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
     1681 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT
     1741 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA
     1801 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
     1861 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA
     1921 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
     1981 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT
     2041 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA
     2101 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
     2161 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
     2221 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
     2281 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA
     2341 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG
     2401 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
     2461 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
     2521 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
     2581 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT
     2641 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT
     2701 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC
     2761 CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT TCAGGTGGAG TGGGAGAAGA
     2821 ATTACTGACC CCTCGGGGTC TCA
//