LOCUS       BBF10K_003448           2786 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003448
VERSION     BBF10K_003448.1  
FEATURES             Location/Qualifiers
     CDS             5..666
                     /label=CD_AmilCP
     CDS             complement(1709..2568)
                     /label=AmpR
     promoter        complement(2570..2673)
                     /label=AmpR promoter
     primer_bind     2692..2708
                     /label=M13 fwd
     primer_bind     complement(847..862)
                     /label=M13 rev
     rep_origin      complement(930..1517)
                     /label=ColE1
     primer_bind     764..782
                     /label=SLiCE-for
     primer_bind     complement(2736..2753)
                     /label=SLiCE-rev
     misc_feature    1521..1710
                     /label=spacer
     CDS             881..931
                     /label=spacer
     terminator      797..844
                     /label=T7 terminator
     terminator      2709..2740
                     /label=tonB terminator
     misc_feature    759..2759
                     /label=pOpen_v3 backbone
     misc_feature    2780..2785
                     /label=BsaI_site
     misc_feature    complement(672..677)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=AATG
     misc_feature    667..670
                     /label=AGGT
ORIGIN
        1 AATGAGCGTA ATTGCGAAAC AAATGACATA TAAAGTTTAT ATGTCAGGTA CGGTTAATGG
       61 TCATTACTTC GAAGTTGAAG GTGACGGTAA GGGTAAACCA TACGAGGGTG AACAAACTGT
      121 TAAATTAACA GTAACAAAAG GTGGCCCTTT ACCATTTGCT TGGGATATCT TATCTCCACA
      181 ATGTCAATAC GGTTCAATCC CATTTACTAA GTATCCAGAA GATATTCCGG ACTATGTAAA
      241 ACAGTCTTTT CCTGAAGGTT ACACTTGGGA ACGTATTATG AACTTCGAGG ACGGTGCAGT
      301 ATGTACGGTA TCTAATGATT CTTCTATTCA GGGTAACTGT TTCATTTATC ATGTAAAATT
      361 TTCTGGTTTA AATTTTCCAC CAAACGGGCC AGTAATGCAA AAAAAAACAC AAGGTTGGGA
      421 ACCAAATACT GAACGTTTAT TCGCACGTGA TGGTATGCTT TTAGGTAACA ACTTTATGGC
      481 GTTGAAATTA GAGGGCGGTG GTCATTACTT GTGTGAGTTC AAGACAACTT ATAAAGCTAA
      541 AAAACCGGTA AAAATGCCAG GTTACCACTA CGTAGATCGT AAATTAGATG TTACGAACCA
      601 TAACAAAGAC TATACTTCTG TAGAACAATG CGAAATTAGT ATTGCTCGCA AACCAGTAGT
      661 TGCCGGAGGT CGAGACCAGA GTATTATCCG AGCGCCTTTG TCACACCTCT CCCTGGGTCT
      721 AGGGATAACA GGGTAATATA GCCTTTTTTA TAAGGCTGCG CTGAGGTGTC AATCGTCGGA
      781 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT
      841 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT
      901 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA
      961 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
     1021 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
     1081 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
     1141 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
     1201 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
     1261 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
     1321 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT
     1381 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
     1441 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
     1501 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT
     1561 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
     1621 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
     1681 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC
     1741 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT
     1801 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC
     1861 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT
     1921 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA
     1981 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG
     2041 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT
     2101 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC
     2161 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT
     2221 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC
     2281 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC
     2341 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA
     2401 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC
     2461 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA
     2521 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT
     2581 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
     2641 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC
     2701 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTTCAGGTG GAGTGGGAGA
     2761 AGAATTACTG ACCCCTCGGG GTCTCA
//