LOCUS BBF10K_003449 2624 bp DNA circular 29-Apr-2025 ACCESSION BBF10K_003449 VERSION BBF10K_003449.1 FEATURES Location/Qualifiers CDS 5..504 /label=CD_TthRNAseH CDS complement(1547..2406) /label=AmpR promoter complement(2408..2511) /label=AmpR promoter primer_bind 2530..2546 /label=M13 fwd primer_bind complement(685..700) /label=M13 rev rep_origin complement(768..1355) /label=ColE1 primer_bind 602..620 /label=SLiCE-for primer_bind complement(2574..2591) /label=SLiCE-rev misc_feature 1359..1548 /label=spacer CDS 719..769 /label=spacer terminator 635..682 /label=T7 terminator terminator 2547..2578 /label=tonB terminator misc_feature 597..2597 /label=pOpen_v3 backbone misc_feature 2618..2623 /label=BsaI_site misc_feature complement(510..515) /label=BsaI_site misc_feature 1..4 /label=AATG misc_feature 505..508 /label=AGGT ORIGIN 1 AATGGTGAAC CCGAGTCCAC GCAAACGCGT AGCGCTATTT ACCGATGGTG CGTGCCTGGG 61 AAATCCGGGT CCCGGAGGTT GGGCTGCGCT GTTAAGATTC CATGCTCATG AGAAACTCCT 121 GTCGGGGGGT GAGGCCTGGA CCACCAACAA CCGCATGGAA CTGAAAGCGG CAATTGAAGG 181 CTTAAAAGCC CTGAAGGAGC CTTGTGAAGT TGATCTCTAC ACAGATAGCC ACTATTTGAA 241 AAAAGCGTTC ACCGAGGGCT GGCTGGAAGG TTGGCGTAAA CGGGGCTGGC GGACTGCCGA 301 GGGCAAGCCG GTCAAAAATC GCGACCTGTG GGAAGCTCTG TTGCTTGCCA TGGCACCTCA 361 TCGTGTGCGC TTCCATTTTG TTAAGGGGCA TACTGGCCAC CCGGAAAATG AACGAGTGGA 421 CCGTGAAGCG CGTCGCCAGG CACAATCCCA GGCGAAAACG CCCTGCCCGC CACGTGCCCC 481 GACGCTTTTT CACGAAGAAG CAGGAGGTCG AGACCAGCGG TCGCTATACA TACCTTCGGT 541 CGAAGAGGAA CGGTAGCTAG GGATAACAGG GTAATGGTGC TGAAAAGCTA GATATGCGCT 601 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA 661 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG 721 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT 781 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 841 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 901 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 961 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 1021 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 1081 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 1141 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 1201 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 1261 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 1321 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC 1381 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG 1441 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA 1501 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA 1561 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC 1621 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG 1681 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA 1741 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT 1801 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT 1861 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC 1921 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC 1981 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA 2041 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG 2101 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG 2161 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA 2221 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA 2281 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA 2341 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA 2401 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 2461 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC 2521 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG 2581 TTCAGGTGGA GTGGGAGAAG AATTACTGAC CCCTCGGGGT CTCA //