LOCUS       BBF10K_003450           2588 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003450
VERSION     BBF10K_003450.1  
FEATURES             Location/Qualifiers
     CDS             5..468
                     /label=CD_EcRNAseH
     CDS             complement(1511..2370)
                     /label=AmpR
     promoter        complement(2372..2475)
                     /label=AmpR promoter
     primer_bind     2494..2510
                     /label=M13 fwd
     primer_bind     complement(649..664)
                     /label=M13 rev
     rep_origin      complement(732..1319)
                     /label=ColE1
     primer_bind     566..584
                     /label=SLiCE-for
     primer_bind     complement(2538..2555)
                     /label=SLiCE-rev
     misc_feature    1323..1512
                     /label=spacer
     CDS             683..733
                     /label=spacer
     terminator      599..646
                     /label=T7 terminator
     terminator      2511..2542
                     /label=tonB terminator
     misc_feature    561..2561
                     /label=pOpen_v3 backbone
     misc_feature    2582..2587
                     /label=BsaI_site
     misc_feature    complement(474..479)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=AATG
     misc_feature    469..472
                     /label=AGGT
ORIGIN
        1 AATGCTTAAA CAGGTAGAAA TTTTCACCGA TGGTTCGTGT CTGGGCAATC CAGGACCTGG
       61 GGGTTACGGC GCTATTTTAC GCTATCGCGG ACGCGAGAAA ACCTTTAGCG CTGGCTACAC
      121 CCGCACCACC AACAACCGTA TGGAGTTGAT GGCCGCTATT GTCGCGCTGG AGGCGTTAAA
      181 AGAACATTGC GAAGTCATTT TGAGTACCGA CAGCCAGTAT GTCCGCCAAG GTATCACCCA
      241 GTGGATCCAT AACTGGAAAA AACGTGGCTG GAAAACCGCA GACAAAAAAC CAGTAAAAAA
      301 TGTCGATCTC TGGCAACGTC TTGATGCTGC ATTGGGGCAG CATCAAATCA AATGGGAATG
      361 GGTTAAAGGC CATGCCGGAC ACCCGGAAAA CGAACGCTGT GATGAACTGG CTCGTGCCGC
      421 GGCGATGAAT CCCACACTGG AAGATACAGG CTACCAAGTT GAAGTTGGAG GTCGAGACCA
      481 CCGCGTAAGA GGTTCCTATT GAATCGACTG AGGTTTCTGC ATAGGGATAA CAGGGTAATC
      541 GCATGAGTTC GCAAGCGAGT CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA
      601 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT
      661 TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA
      721 GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
      781 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
      841 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
      901 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
      961 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
     1021 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
     1081 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
     1141 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
     1201 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     1261 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     1321 AGAAGGCCTA CTATTAGCAA CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG
     1381 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC
     1441 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT
     1501 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT
     1561 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA
     1621 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA
     1681 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC
     1741 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT
     1801 AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT
     1861 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG
     1921 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA
     1981 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA
     2041 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG
     2101 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT
     2161 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG
     2221 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT
     2281 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA
     2341 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC
     2401 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA
     2461 CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC
     2521 TCCGACCGGA GGCTTTTGAC TTGGTTCAGG TGGAGTGGGA GAAGAATTAC TGACCCCTCG
     2581 GGGTCTCA
//