LOCUS       BBF10K_003451           2735 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003451
VERSION     BBF10K_003451.1  
FEATURES             Location/Qualifiers
     CDS             5..615
                     /label=DT4_Int_SaltS
     CDS             complement(1658..2517)
                     /label=AmpR
     promoter        complement(2519..2622)
                     /label=AmpR promoter
     primer_bind     2641..2657
                     /label=M13 fwd
     primer_bind     complement(796..811)
                     /label=M13 rev
     rep_origin      complement(879..1466)
                     /label=ColE1
     primer_bind     713..731
                     /label=SLiCE-for
     primer_bind     complement(2685..2702)
                     /label=SLiCE-rev
     misc_feature    1470..1659
                     /label=spacer
     CDS             830..880
                     /label=spacer
     terminator      746..793
                     /label=T7 terminator
     terminator      2658..2689
                     /label=tonB terminator
     misc_feature    708..2708
                     /label=pOpen_v3 backbone
     misc_feature    2729..2734
                     /label=BsaI_site
     misc_feature    complement(621..626)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=AGGT
     misc_feature    616..619
                     /label=TTCG
ORIGIN
        1 AGGTGGCAGC AGTGGCAGCG CGGCGGTGGA TGAGCTGGAT AAAATGAGAT GCGTTACGGG
       61 AGATACCCTG GTACAGGCGG GCGATGGCCG CCGTCGCATT CGCGAATTGG CGGGCGAAAC
      121 GGCGGAAGCC GGTTCAATAG AGGAATTACC GAACGGGCGG ACCATCCGCG ACGTTGATAT
      181 TGACGTGTGG ACGATGACCG ATGACGAAAC CTTGACCCGT CGTCCAGTCA CAGCAATCCA
      241 TGAATACGAC GCGCCTGAGA CTCTGTATGA GGTGACCCTG TCGACTGGCG AAGAAGTCAC
      301 CGTTACACCG GATCACCCGT TCTTCATCGA ACAGGCATCC GGTCGAGTCG AGACTCCGGC
      361 TGAGGATCTG CAACCGGGGG ATCTGGTGTT TGTGCCAGAA GGTTCCGCCA TGGCCACGGA
      421 TGGCGGGATT GCTCAGATTG ACACAAGTTC TGATCGCCTT GGACCTGCAG AATCTGGACT
      481 GGGTGACATC GGCCTCCGTA CGATTGAAAA CGTGGAATCG GTTCCCGACC ATGACTACGA
      541 TAGCGTCTAT GATTTAACCG TAGAAGGCAC GCACAATTTT CTAGCTAATG GTATGGTAGT
      601 TCATGCCGCA GGTGGTTCGC GAGACCACGG TGCTTTTGCG TGATATTGGG GTTCAGGCAA
      661 CTTCGCACTA GGGATAACAG GGTAATCCTC TTATCCAATA GTCGTAGCGC TGAGGTGTCA
      721 ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG
      781 AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT GACACACATT
      841 AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC TCCGCCCCCC
      901 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
      961 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
     1021 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
     1081 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
     1141 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
     1201 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
     1261 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
     1321 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
     1381 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
     1441 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGGCCTACTA TTAGCAACAA CGATCCTTTG
     1501 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC
     1561 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA
     1621 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG
     1681 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG
     1741 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA
     1801 GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG
     1861 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA
     1921 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC
     1981 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA
     2041 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG
     2101 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT
     2161 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC
     2221 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG
     2281 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG
     2341 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT
     2401 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA
     2461 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA
     2521 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC
     2581 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG CACCAGTCAG
     2641 TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG GTTCAGGTGG
     2701 AGTGGGAGAA GAATTACTGA CCCCTCGGGG TCTCA
//