LOCUS       BBF10K_003452           2723 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003452
VERSION     BBF10K_003452.1  
FEATURES             Location/Qualifiers
     CDS             5..603
                     /label=DT4_Int_ThiolS
     CDS             complement(1646..2505)
                     /label=AmpR
     promoter        complement(2507..2610)
                     /label=AmpR promoter
     primer_bind     2629..2645
                     /label=M13 fwd
     primer_bind     complement(784..799)
                     /label=M13 rev
     rep_origin      complement(867..1454)
                     /label=ColE1
     primer_bind     701..719
                     /label=SLiCE-for
     primer_bind     complement(2673..2690)
                     /label=SLiCE-rev
     misc_feature    1458..1647
                     /label=spacer
     CDS             818..868
                     /label=spacer
     terminator      734..781
                     /label=T7 terminator
     terminator      2646..2677
                     /label=tonB terminator
     misc_feature    696..2696
                     /label=pOpen_v3 backbone
     misc_feature    2717..2722
                     /label=BsaI_site
     misc_feature    complement(609..614)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=AGGT
     misc_feature    604..607
                     /label=TTCG
ORIGIN
        1 AGGTTGTATT ACCGGCGATG CCCTAGTGGC CCTGCCTGAG GGCGAATCCG TTCGCATTGC
       61 AGACATCGTG CCTGGCGCCC GTCCCAACAG CGATAATGCG ATAGACCTGA AGGTGCTTGA
      121 TAGACACGGG AACCCGGTGC TGGCGGACCG CCTCTTCCAC AGTGGTGAGC ATCCAGTTTA
      181 CACAGTCCGA ACGGTGGAAG GGCTGCGCGT GACCGGCACA GCAAACCATC CGTTGTTGTG
      241 CCTGGTAGAT GTTGCTGGAG TACCAACTCT CCTGTGGAAA CTGATCGATG AAATCAAACC
      301 GGGTGATTAT GCGGTCATTC AGCGTAGCGC CTTTAGTGTA GATTGCGCCG GTTTCGCGCG
      361 TGGGAAACCG GAATTTGCTC CGACCACGTA TACGGTCGGC GTTCCCGGAC TGGTCCGCTT
      421 TCTTGAAGCC CATCATCGGG ACCCGGATGC ACAAGCAATT GCGGACGAGT TAACGGATGG
      481 TCGCTTTTAT TACGCGAAAG TTGCGTCTGT CACTGACGCG GGTGTGCAGC CGGTATACTC
      541 ATTACGCGTT GATACCGCAG ACCATGCTTT CATCACCAAT GGCTTTGTGT CGCACGCTGG
      601 CGGTTCGCGA GACCAGGCTG CCAAAGAAAC TGATTGACGG GTACCCTCTG CTGGATTAGG
      661 GATAACAGGG TAATCTTTCT TCGTGGCCCT AATCACGCTG AGGTGTCAAT CGTCGGAGCC
      721 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG
      781 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT
      841 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
      901 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
      961 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     1021 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     1081 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     1141 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     1201 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     1261 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
     1321 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     1381 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     1441 AAAAAAAGGA TCTCAAGAAG GCCTACTATT AGCAACAACG ATCCTTTGAT CTTTTCTACG
     1501 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
     1561 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT
     1621 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA
     1681 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
     1741 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA
     1801 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
     1861 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT
     1921 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA
     1981 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
     2041 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
     2101 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
     2161 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA
     2221 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG
     2281 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
     2341 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
     2401 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
     2461 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT
     2521 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT
     2581 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC
     2641 CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT TCAGGTGGAG TGGGAGAAGA
     2701 ATTACTGACC CCTCGGGGTC TCA
//