LOCUS BBF10K_003453 2409 bp DNA circular 29-Apr-2025 ACCESSION BBF10K_003453 VERSION BBF10K_003453.1 FEATURES Location/Qualifiers CDS 5..39 /label=DT4_TEV CDS complement(1207..2066) /label=AmpR promoter complement(2068..2171) /label=AmpR promoter primer_bind 2190..2206 /label=M13 fwd primer_bind complement(345..360) /label=M13 rev rep_origin complement(428..1015) /label=ColE1 primer_bind 262..280 /label=SLiCE-for primer_bind complement(2234..2251) /label=SLiCE-rev misc_feature 1019..1208 /label=spacer CDS 379..429 /label=spacer terminator 295..342 /label=T7 terminator terminator 2207..2238 /label=tonB terminator misc_feature 257..2257 /label=pOpen_v3 backbone misc_feature 2403..2408 /label=BsaI_site misc_feature complement(45..50) /label=BsaI_site misc_feature 1..4 /label=AGGT misc_feature 40..43 /label=TTCG ORIGIN 1 AGGTAGCGGC GGAAGCGAAA ATCTTTATTT TCAAGGTGGT TCGCGAGACC CGTATCAACG 61 CGCAGGCTTA GTTCGAGATA AAATGTCCAG AGCCAAAGAC AACCAGCATT TCGCGTCTTG 121 CCTAATCCCC CTACATGTTG TTATAAATAA TCAGTAGAAA CTCAGTGTTA GACGAACGCG 181 CTAATAGCTT TGCATTTTAT CGCTTTGGCG CATGGTATAG GGATAACAGG GTAATTTTAA 241 TAGAACATAT GGTCGCCGCT GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT 301 AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC 361 TGAGAGCTTG GCAGGTGATG ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT 421 GCCATTAATT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT 481 CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC 541 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT 601 TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC 661 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA 721 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA 781 GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG 841 TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG 901 CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT 961 AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA 1021 GGCCTACTAT TAGCAACAAC GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 1081 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 1141 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT 1201 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT 1261 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT 1321 CTGGCCCCAG TGCTGCAATG ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG 1381 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT 1441 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT 1501 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG 1561 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA 1621 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT 1681 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT 1741 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC 1801 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA 1861 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT 1921 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT 1981 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA 2041 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT 2101 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA 2161 TAGGGGTTCC GCGCACCTGC ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG 2221 ACCGGAGGCT TTTGACTTGG TTCAGGTGGA GTGGGAGAAG AATTACTGAC CCCTCGGCCT 2281 AGCGTACAGG GTGCACTTTG TAACGATTTG GGAGTCCAAA GACTCGCTGT TTTCGAAATT 2341 TCCCTTTAAG CGCGAGTATT GAACCAGGCT TATGCCCAAG ATCGTAGCAA GCAGACTCAA 2401 ACGGTCTCA //