LOCUS       BBF10K_003454           2852 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003454
VERSION     BBF10K_003454.1  
FEATURES             Location/Qualifiers
     CDS             5..732
                     /label=DT4_fuGFP
     CDS             complement(1775..2634)
                     /label=AmpR
     promoter        complement(2636..2739)
                     /label=AmpR promoter
     primer_bind     2758..2774
                     /label=M13 fwd
     primer_bind     complement(913..928)
                     /label=M13 rev
     rep_origin      complement(996..1583)
                     /label=ColE1
     primer_bind     830..848
                     /label=SLiCE-for
     primer_bind     complement(2802..2819)
                     /label=SLiCE-rev
     misc_feature    1587..1776
                     /label=spacer
     CDS             947..997
                     /label=spacer
     terminator      863..910
                     /label=T7 terminator
     terminator      2775..2806
                     /label=tonB terminator
     misc_feature    825..2825
                     /label=pOpen_v3 backbone
     misc_feature    2846..2851
                     /label=BsaI_site
     misc_feature    complement(738..743)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=AGGT
     misc_feature    733..736
                     /label=TTCG
ORIGIN
        1 AGGTGGCGGT TCGGTTTCTA GCGGTGAAGA TATCTTCTCA GGCCTGGTCC CGATTCTGAT
       61 CGAGCTGGAG GGCGATGTGA ATGGCCACCG CTTCTCTGTT CGTGGTGAAG GTTATGGCGA
      121 CGCCTCTAAC GGCAAACTGG AAATTAAGTT CATCTGCACC ACCGGTCGTC TGCCGGTTCC
      181 GTGGCCGACG CTGGTGACTA CTCTGTCTTA CGGTGTCCAG TGCTTCGCAA AATACCCGGA
      241 ACACATGCGT CAGAATGATT TTTTCAAGTC CGCCATGCCA GATGGCTATG TTCAGGAACG
      301 TACCATCAGC TTCAAAGAGG ACGGTACGTA CAAAACCCGT GCGGAAGTTA AATTTGAAGG
      361 TGAAGCGCTG GTGAACCGTA TCGACTTAAA AGGTCTGGAG TTCAAGGAAG ATGGCAACAT
      421 TCTGGGCCAC AAACTGGAAT ACAGCTTCAA CAGCCACTAT GTTTATATTA CCGCGGACAA
      481 AAATCGCAAC GGCCTGGAGG CGCAGTTCCG TATCCGTCAC AACGTCGACG ACGGTTCTGT
      541 CCAGCTTGCG GACCATTACC AGCAGAACAC CCCGATCGGT GAGGGCCCAG TACTGCTCCC
      601 GGAGCAACAC TACCTGACGA CTAACTCTGT CCTGTCCAAA GACCCGCAGG AACGCCGTGA
      661 TCACATGGTT CTGGTTGAAT TTGTGACCGC TGCCGGCCTG TCGCTGGGCA TGGACGAACT
      721 GTATAAATCC GGTTCGCGAG ACCAGCCGTG TAAAATGACG CATTGTGGGG TAGCGTTCGA
      781 CGTACTAGGG ATAACAGGGT AATACACCGG ACCGATTCAA TGATCGCTGA GGTGTCAATC
      841 GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG
      901 GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC
      961 AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA
     1021 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
     1081 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
     1141 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
     1201 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
     1261 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
     1321 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
     1381 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC
     1441 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
     1501 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
     1561 TACGCGCAGA AAAAAAGGAT CTCAAGAAGG CCTACTATTA GCAACAACGA TCCTTTGATC
     1621 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG
     1681 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA
     1741 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA
     1801 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG
     1861 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA
     1921 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC
     1981 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT
     2041 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC
     2101 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG
     2161 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC
     2221 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT
     2281 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG
     2341 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT
     2401 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG
     2461 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA
     2521 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA
     2581 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC
     2641 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
     2701 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA
     2761 AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTT CAGGTGGAGT
     2821 GGGAGAAGAA TTACTGACCC CTCGGGGTCT CA
//