LOCUS       BBF10K_003457           2424 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003457
VERSION     BBF10K_003457.1  
FEATURES             Location/Qualifiers
     CDS             5..24
                     /label=DT5_TEVcut
     CDS             complement(1207..2066)
                     /label=AmpR
     promoter        complement(2068..2171)
                     /label=AmpR promoter
     primer_bind     2190..2206
                     /label=M13 fwd
     primer_bind     complement(345..360)
                     /label=M13 rev
     rep_origin      complement(428..1015)
                     /label=ColE1
     primer_bind     262..280
                     /label=SLiCE-for
     primer_bind     complement(2234..2251)
                     /label=SLiCE-rev
     misc_feature    1019..1208
                     /label=spacer
     CDS             379..429
                     /label=spacer
     terminator      295..342
                     /label=T7 terminator
     terminator      2207..2238
                     /label=tonB terminator
     misc_feature    257..2257
                     /label=pOpen_v3 backbone
     misc_feature    2418..2423
                     /label=BsaI_site
     misc_feature    complement(30..35)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=AGGT
     misc_feature    25..28
                     /label=CGGC
ORIGIN
        1 AGGTGAAAAT CTTTATTTTC AAGGCGGCCG AGACCAAGTG CTGGATCCTA TTCCAGCGGG
       61 ATCTTTTATC TAAAGACAAT GAGAGGAGTA TTCGTCAGGC CACATAGCTT TCATGTTCTG
      121 ATCGGAACGA TCGTTGGCGC CCGATCCTCG GATTCCATAG TGAGTTCTTT GTCTGAGTGA
      181 TATGCCGACT CCTTGTTGGC AGGCGCGCAC GAGTTAATAG GGATAACAGG GTAATCCCGG
      241 CCATTCATTT GAGGCTCGCT GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT
      301 AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC
      361 TGAGAGCTTG GCAGGTGATG ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT
      421 GCCATTAATT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT
      481 CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC
      541 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT
      601 TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC
      661 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA
      721 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA
      781 GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG
      841 TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG
      901 CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT
      961 AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA
     1021 GGCCTACTAT TAGCAACAAC GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA
     1081 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA
     1141 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT
     1201 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT
     1261 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT
     1321 CTGGCCCCAG TGCTGCAATG ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG
     1381 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT
     1441 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT
     1501 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG
     1561 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA
     1621 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT
     1681 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT
     1741 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC
     1801 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA
     1861 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT
     1921 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT
     1981 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA
     2041 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT
     2101 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA
     2161 TAGGGGTTCC GCGCACCTGC ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG
     2221 ACCGGAGGCT TTTGACTTGG TTCAGGTGGA GTGGGAGAAG AATTACTGAC CCCTCGGAGT
     2281 CATTGTATGC GAGATCGATA GACTGATAGG GGATGCAGTA TATCCCTGGA TACAATAGAC
     2341 GAACAGGTTG GAATCTTAAG TGAAATCGCG CGTCCAAACC CAGCTCTATT TTAGAGGTCA
     2401 TGGGTTCTGG TGCCCGCGGT CTCA
//