LOCUS       BBF10K_003459           2495 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003459
VERSION     BBF10K_003459.1  
FEATURES             Location/Qualifiers
     terminator      322..369
                     /label=T7 terminator
     CDS             5..375
                     /label=DF_TEVcut_His_Stop_TZ
     CDS             complement(1418..2277)
                     /label=AmpR
     promoter        complement(2279..2382)
                     /label=AmpR promoter
     primer_bind     2401..2417
                     /label=M13 fwd
     primer_bind     complement(556..571)
                     /label=M13 rev
     rep_origin      complement(639..1226)
                     /label=ColE1
     primer_bind     473..491
                     /label=SLiCE-for
     primer_bind     complement(2445..2462)
                     /label=SLiCE-rev
     misc_feature    1230..1419
                     /label=spacer
     CDS             590..640
                     /label=spacer
     terminator      506..553
                     /label=T7 terminator
     terminator      2418..2449
                     /label=tonB terminator
     misc_feature    468..2468
                     /label=pOpen_v3 backbone
     misc_feature    2489..2494
                     /label=BsaI_site
     misc_feature    complement(381..386)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=AGGT
     misc_feature    376..379
                     /label=CGCT
ORIGIN
        1 AGGTAGCGGC GGAGAAAATC TTTATTTTCA AGGTGGAAGC CATCACCATC ACCATCACAG
       61 CAGCGGCTAA TAAGGATCCG AATTCGAGCT CCGTCGACCT AGCATAACCC CGCCGGGCCT
      121 CTTCGGGGGT CTGGCGGGGT TTTTTGCTGA AAGAAGCTTC AAATAAAACG AAAGGCTCAG
      181 TCGAAAGACT GGGCCTTTCG TTTTATCTGT TGTTTGTCGC TGCGGCCGCA CTCGAGCACC
      241 ACCACCACCA CCACTGAGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG
      301 CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG
      361 GTTTTTTGCT GAAAGCGCTC GAGACCAGCG TATACCTTGC TTCCTGTCAT TAGACCACCT
      421 GGCGTACGTA GGGATAACAG GGTAATGAGT TCGCACCAAG TCGAAAACGC TGAGGTGTCA
      481 ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG
      541 AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT GACACACATT
      601 AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC TCCGCCCCCC
      661 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
      721 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
      781 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
      841 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
      901 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
      961 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
     1021 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
     1081 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
     1141 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
     1201 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGGCCTACTA TTAGCAACAA CGATCCTTTG
     1261 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC
     1321 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA
     1381 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG
     1441 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG
     1501 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA
     1561 GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG
     1621 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA
     1681 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC
     1741 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA
     1801 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG
     1861 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT
     1921 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC
     1981 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG
     2041 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG
     2101 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT
     2161 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA
     2221 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA
     2281 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC
     2341 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG CACCAGTCAG
     2401 TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG GTTCAGGTGG
     2461 AGTGGGAGAA GAATTACTGA CCCCTCGGGG TCTCA
//