LOCUS BBF10K_003460 3218 bp DNA circular 29-Apr-2025 ACCESSION BBF10K_003460 VERSION BBF10K_003460.1 FEATURES Location/Qualifiers terminator 1045..1092 /label=T7 terminator CDS 5..1098 /label=DF_TEVcut_fuGFP_His_Stop_TZ CDS complement(2141..3000) /label=AmpR promoter complement(3002..3105) /label=AmpR promoter primer_bind 3124..3140 /label=M13 fwd primer_bind complement(1279..1294) /label=M13 rev rep_origin complement(1362..1949) /label=ColE1 primer_bind 1196..1214 /label=SLiCE-for primer_bind complement(3168..3185) /label=SLiCE-rev misc_feature 1953..2142 /label=spacer CDS 1313..1363 /label=spacer terminator 1229..1276 /label=T7 terminator terminator 3141..3172 /label=tonB terminator misc_feature 1191..3191 /label=pOpen_v3 backbone misc_feature 3212..3217 /label=BsaI_site misc_feature complement(1104..1109) /label=BsaI_site misc_feature 1..4 /label=AGGT misc_feature 1099..1102 /label=CGCT ORIGIN 1 AGGTAGCGGC GGAGAAAATC TTTATTTTCA AGGTGGAAGC GGTGGCTCGG TTTCTAGCGG 61 TGAAGATATC TTCTCAGGCC TGGTCCCGAT TCTGATCGAG CTGGAGGGCG ATGTGAATGG 121 CCACCGCTTC TCTGTTCGTG GTGAAGGTTA TGGCGACGCC TCTAACGGCA AACTGGAAAT 181 TAAGTTCATC TGCACCACCG GTCGTCTGCC GGTTCCGTGG CCGACGCTGG TGACTACTCT 241 GTCTTACGGT GTCCAGTGCT TCGCAAAATA CCCGGAACAC ATGCGTCAGA ATGATTTTTT 301 CAAGTCCGCC ATGCCAGATG GCTATGTTCA GGAACGTACC ATCAGCTTCA AAGAGGACGG 361 TACGTACAAA ACCCGTGCGG AAGTTAAATT TGAAGGTGAA GCGCTGGTGA ACCGTATCGA 421 CTTAAAAGGT CTGGAGTTCA AGGAAGATGG CAACATTCTG GGCCACAAAC TGGAATACAG 481 CTTCAACAGC CACTATGTTT ATATTACCGC GGACAAAAAT CGCAACGGCC TGGAGGCGCA 541 GTTCCGTATC CGTCACAACG TCGACGACGG TTCTGTCCAG CTTGCGGACC ATTACCAGCA 601 GAACACCCCG ATCGGTGAGG GCCCAGTACT GCTCCCGGAG CAACACTACC TGACGACTAA 661 CTCTGTCCTG TCCAAAGACC CGCAGGAACG CCGTGATCAC ATGGTTCTGG TTGAATTTGT 721 GACCGCTGCC GGCCTGTCGC TGGGCATGGA CGAACTGTAT AAATCCGGCG GACATCACCA 781 TCACCATCAC TAATAAGGAT CCGAATTCGA GCTCCGTCGA CCTAGCATAA CCCCGCCGGG 841 CCTCTTCGGG GGTCTGGCGG GGTTTTTTGC TGAAAGAAGC TTCAAATAAA ACGAAAGGCT 901 CAGTCGAAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGCTGCGGCC GCACTCGAGC 961 ACCACCACCA CCACCACTGA GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG 1021 CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA 1081 GGGGTTTTTT GCTGAAAGCG CTCGAGACCA GGGCACTCAA TATACCCTGT ACACATAGTT 1141 ACACCCCATT CTAGGGATAA CAGGGTAATC TTATTGTGTC GGCGATTTTT CGCTGAGGTG 1201 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC 1261 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC 1321 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC 1381 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 1441 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 1501 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 1561 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 1621 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 1681 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 1741 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 1801 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 1861 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 1921 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA CAACGATCCT 1981 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG 2041 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT 2101 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT 2161 GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC 2221 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG 2281 CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC 2341 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG 2401 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA 2461 GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA 2521 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT 2581 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG 2641 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA 2701 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA 2761 CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT 2821 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT 2881 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA 2941 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC 3001 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 3061 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT 3121 CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTTCAGG 3181 TGGAGTGGGA GAAGAATTAC TGACCCCTCG GGGTCTCA //