BBF10K_003460
1
DF_TEVcut_fuGFP_His_Stop_TZ
BBF10K_003460_part_comp
1
BBF10K_003498_comp
1
BBF10K_003498_annot
1
BBF10K_003498_range
1
1191
3191
BBF10K_003460_part_annot
1
BBF10K_003460_part_range
1
5
1098
BsaI_site_fw_annotation
1
BsaI_site
BsaI_site_fw_annotation_range
1
3212
3217
three_prime_fusion
1
CGCT
three_prime_fusion_range
1
1099
1102
BsaI_site_rv_annotation
1
BsaI_site
BsaI_site_rv_annotation_range
1
1104
1109
five_prime_fusion
1
AGGT
five_prime_fusion_range
1
1
4
BBF10K_003498_backbone
1
pOpen_v3 backbone
annotation7
1
spacer
annotation7_range
1
763
952
annotation0
1
AmpR
annotation0_range
1
951
1810
annotation9
1
T7 terminator
annotation9_range
1
39
86
annotation5
1
SLiCE-for
annotation5_range
1
6
24
annotation1
1
AmpR promoter
annotation1_range
1
1812
1915
annotation4
1
ColE1
annotation4_range
1
172
759
annotation10
1
tonB terminator
annotation10_range
1
1951
1982
annotation8
1
spacer
annotation8_range
1
123
173
annotation6
1
SLiCE-rev
annotation6_range
1
1978
1995
annotation2
1
M13 fwd
annotation2_range
1
1934
1950
annotation3
1
M13 rev
annotation3_range
1
89
104
BBF10K_003460_part
1
DF_TEVcut_fuGFP_His_Stop_TZ
annotation0
1
T7 terminator
annotation0_range
1
1041
1088
BBF10K_003460_seq
1
AGGTAGCGGCGGAGAAAATCTTTATTTTCAAGGTGGAAGCGGTGGCTCGGTTTCTAGCGGTGAAGATATCTTCTCAGGCCTGGTCCCGATTCTGATCGAGCTGGAGGGCGATGTGAATGGCCACCGCTTCTCTGTTCGTGGTGAAGGTTATGGCGACGCCTCTAACGGCAAACTGGAAATTAAGTTCATCTGCACCACCGGTCGTCTGCCGGTTCCGTGGCCGACGCTGGTGACTACTCTGTCTTACGGTGTCCAGTGCTTCGCAAAATACCCGGAACACATGCGTCAGAATGATTTTTTCAAGTCCGCCATGCCAGATGGCTATGTTCAGGAACGTACCATCAGCTTCAAAGAGGACGGTACGTACAAAACCCGTGCGGAAGTTAAATTTGAAGGTGAAGCGCTGGTGAACCGTATCGACTTAAAAGGTCTGGAGTTCAAGGAAGATGGCAACATTCTGGGCCACAAACTGGAATACAGCTTCAACAGCCACTATGTTTATATTACCGCGGACAAAAATCGCAACGGCCTGGAGGCGCAGTTCCGTATCCGTCACAACGTCGACGACGGTTCTGTCCAGCTTGCGGACCATTACCAGCAGAACACCCCGATCGGTGAGGGCCCAGTACTGCTCCCGGAGCAACACTACCTGACGACTAACTCTGTCCTGTCCAAAGACCCGCAGGAACGCCGTGATCACATGGTTCTGGTTGAATTTGTGACCGCTGCCGGCCTGTCGCTGGGCATGGACGAACTGTATAAATCCGGCGGACATCACCATCACCATCACTAATAAGGATCCGAATTCGAGCTCCGTCGACCTAGCATAACCCCGCCGGGCCTCTTCGGGGGTCTGGCGGGGTTTTTTGCTGAAAGAAGCTTCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGCTGCGGCCGCACTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGCGCTCGAGACCAGGGCACTCAATATACCCTGTACACATAGTTACACCCCATTCTAGGGATAACAGGGTAATCTTATTGTGTCGGCGATTTTTCGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAAGAATTACTGACCCCTCGGGGTCTCA
BBF10K_003460_part_seq
1
AGCGGCGGAGAAAATCTTTATTTTCAAGGTGGAAGCGGTGGCTCGGTTTCTAGCGGTGAAGATATCTTCTCAGGCCTGGTCCCGATTCTGATCGAGCTGGAGGGCGATGTGAATGGCCACCGCTTCTCTGTTCGTGGTGAAGGTTATGGCGACGCCTCTAACGGCAAACTGGAAATTAAGTTCATCTGCACCACCGGTCGTCTGCCGGTTCCGTGGCCGACGCTGGTGACTACTCTGTCTTACGGTGTCCAGTGCTTCGCAAAATACCCGGAACACATGCGTCAGAATGATTTTTTCAAGTCCGCCATGCCAGATGGCTATGTTCAGGAACGTACCATCAGCTTCAAAGAGGACGGTACGTACAAAACCCGTGCGGAAGTTAAATTTGAAGGTGAAGCGCTGGTGAACCGTATCGACTTAAAAGGTCTGGAGTTCAAGGAAGATGGCAACATTCTGGGCCACAAACTGGAATACAGCTTCAACAGCCACTATGTTTATATTACCGCGGACAAAAATCGCAACGGCCTGGAGGCGCAGTTCCGTATCCGTCACAACGTCGACGACGGTTCTGTCCAGCTTGCGGACCATTACCAGCAGAACACCCCGATCGGTGAGGGCCCAGTACTGCTCCCGGAGCAACACTACCTGACGACTAACTCTGTCCTGTCCAAAGACCCGCAGGAACGCCGTGATCACATGGTTCTGGTTGAATTTGTGACCGCTGCCGGCCTGTCGCTGGGCATGGACGAACTGTATAAATCCGGCGGACATCACCATCACCATCACTAATAAGGATCCGAATTCGAGCTCCGTCGACCTAGCATAACCCCGCCGGGCCTCTTCGGGGGTCTGGCGGGGTTTTTTGCTGAAAGAAGCTTCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGCTGCGGCCGCACTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAG
BBF10K_003498_backbone_seq
1
CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAG