LOCUS       BBF10K_003461           2720 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003461
VERSION     BBF10K_003461.1  
FEATURES             Location/Qualifiers
     CDS             5..600
                     /label=T4T5_Int_ThiolS
     CDS             complement(1643..2502)
                     /label=AmpR
     promoter        complement(2504..2607)
                     /label=AmpR promoter
     primer_bind     2626..2642
                     /label=M13 fwd
     primer_bind     complement(781..796)
                     /label=M13 rev
     rep_origin      complement(864..1451)
                     /label=ColE1
     primer_bind     698..716
                     /label=SLiCE-for
     primer_bind     complement(2670..2687)
                     /label=SLiCE-rev
     misc_feature    1455..1644
                     /label=spacer
     CDS             815..865
                     /label=spacer
     terminator      731..778
                     /label=T7 terminator
     terminator      2643..2674
                     /label=tonB terminator
     misc_feature    693..2693
                     /label=pOpen_v3 backbone
     misc_feature    2714..2719
                     /label=BsaI_site
     misc_feature    complement(606..611)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=TTCG
     misc_feature    601..604
                     /label=CGGC
ORIGIN
        1 TTCGTGTATT ACCGGCGATG CCCTAGTGGC CCTGCCTGAG GGCGAATCCG TTCGCATTGC
       61 AGACATCGTG CCTGGCGCCC GTCCCAACAG CGATAATGCG ATAGACCTGA AGGTGCTTGA
      121 TAGACACGGG AACCCGGTGC TGGCGGACCG CCTCTTCCAC AGTGGTGAGC ATCCAGTTTA
      181 CACAGTCCGA ACGGTGGAAG GGCTGCGCGT GACCGGCACA GCAAACCATC CGTTGTTGTG
      241 CCTGGTAGAT GTTGCTGGAG TACCAACTCT CCTGTGGAAA CTGATCGATG AAATCAAACC
      301 GGGTGATTAT GCGGTCATTC AGCGTAGCGC CTTTAGTGTA GATTGCGCCG GTTTCGCGCG
      361 TGGGAAACCG GAATTTGCTC CGACCACGTA TACGGTCGGC GTTCCCGGAC TGGTCCGCTT
      421 TCTTGAAGCC CATCATCGGG ACCCGGATGC ACAAGCAATT GCGGACGAGT TAACGGATGG
      481 TCGCTTTTAT TACGCGAAAG TTGCGTCTGT CACTGACGCG GGTGTGCAGC CGGTATACTC
      541 ATTACGCGTT GATACCGCAG ACCATGCTTT CATCACCAAT GGCTTTGTGT CGCACGCTGG
      601 CGGCCGAGAC CAGACCTTGG GATCGCTTAT GTGTACAGTT GCAAGGTGTG AGATAGGGAT
      661 AACAGGGTAA TAATAGCTCT TTGGATAAAA TCCGCTGAGG TGTCAATCGT CGGAGCCGCT
      721 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT
      781 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG
      841 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA
      901 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
      961 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
     1021 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
     1081 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
     1141 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
     1201 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
     1261 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT
     1321 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
     1381 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
     1441 AAAAGGATCT CAAGAAGGCC TACTATTAGC AACAACGATC CTTTGATCTT TTCTACGGGG
     1501 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
     1561 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
     1621 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
     1681 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA
     1741 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG
     1801 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT
     1861 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT
     1921 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
     1981 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA
     2041 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
     2101 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC
     2161 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA
     2221 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
     2281 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA
     2341 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT
     2401 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC
     2461 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA
     2521 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
     2581 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG
     2641 TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTTCA GGTGGAGTGG GAGAAGAATT
     2701 ACTGACCCCT CGGGGTCTCA
//