BBF10K_003461 1 T4T5_Int_ThiolS BBF10K_003498_comp 1 BBF10K_003461_part_comp 1 three_prime_fusion 1 CGGC three_prime_fusion_range 1 601 604 BsaI_site_fw_annotation 1 BsaI_site BsaI_site_fw_annotation_range 1 2714 2719 five_prime_fusion 1 TTCG five_prime_fusion_range 1 1 4 BBF10K_003498_annot 1 BBF10K_003498_range 1 693 2693 BsaI_site_rv_annotation 1 BsaI_site BsaI_site_rv_annotation_range 1 606 611 BBF10K_003461_part_annot 1 BBF10K_003461_part_range 1 5 600 BBF10K_003498_backbone 1 pOpen_v3 backbone annotation7 1 spacer annotation7_range 1 763 952 annotation0 1 AmpR annotation0_range 1 951 1810 annotation9 1 T7 terminator annotation9_range 1 39 86 annotation5 1 SLiCE-for annotation5_range 1 6 24 annotation1 1 AmpR promoter annotation1_range 1 1812 1915 annotation4 1 ColE1 annotation4_range 1 172 759 annotation10 1 tonB terminator annotation10_range 1 1951 1982 annotation8 1 spacer annotation8_range 1 123 173 annotation6 1 SLiCE-rev annotation6_range 1 1978 1995 annotation2 1 M13 fwd annotation2_range 1 1934 1950 annotation3 1 M13 rev annotation3_range 1 89 104 BBF10K_003461_part 1 T4T5_Int_ThiolS BBF10K_003461_part_seq 1 TGTATTACCGGCGATGCCCTAGTGGCCCTGCCTGAGGGCGAATCCGTTCGCATTGCAGACATCGTGCCTGGCGCCCGTCCCAACAGCGATAATGCGATAGACCTGAAGGTGCTTGATAGACACGGGAACCCGGTGCTGGCGGACCGCCTCTTCCACAGTGGTGAGCATCCAGTTTACACAGTCCGAACGGTGGAAGGGCTGCGCGTGACCGGCACAGCAAACCATCCGTTGTTGTGCCTGGTAGATGTTGCTGGAGTACCAACTCTCCTGTGGAAACTGATCGATGAAATCAAACCGGGTGATTATGCGGTCATTCAGCGTAGCGCCTTTAGTGTAGATTGCGCCGGTTTCGCGCGTGGGAAACCGGAATTTGCTCCGACCACGTATACGGTCGGCGTTCCCGGACTGGTCCGCTTTCTTGAAGCCCATCATCGGGACCCGGATGCACAAGCAATTGCGGACGAGTTAACGGATGGTCGCTTTTATTACGCGAAAGTTGCGTCTGTCACTGACGCGGGTGTGCAGCCGGTATACTCATTACGCGTTGATACCGCAGACCATGCTTTCATCACCAATGGCTTTGTGTCGCACGCTGG BBF10K_003461_seq 1 TTCGTGTATTACCGGCGATGCCCTAGTGGCCCTGCCTGAGGGCGAATCCGTTCGCATTGCAGACATCGTGCCTGGCGCCCGTCCCAACAGCGATAATGCGATAGACCTGAAGGTGCTTGATAGACACGGGAACCCGGTGCTGGCGGACCGCCTCTTCCACAGTGGTGAGCATCCAGTTTACACAGTCCGAACGGTGGAAGGGCTGCGCGTGACCGGCACAGCAAACCATCCGTTGTTGTGCCTGGTAGATGTTGCTGGAGTACCAACTCTCCTGTGGAAACTGATCGATGAAATCAAACCGGGTGATTATGCGGTCATTCAGCGTAGCGCCTTTAGTGTAGATTGCGCCGGTTTCGCGCGTGGGAAACCGGAATTTGCTCCGACCACGTATACGGTCGGCGTTCCCGGACTGGTCCGCTTTCTTGAAGCCCATCATCGGGACCCGGATGCACAAGCAATTGCGGACGAGTTAACGGATGGTCGCTTTTATTACGCGAAAGTTGCGTCTGTCACTGACGCGGGTGTGCAGCCGGTATACTCATTACGCGTTGATACCGCAGACCATGCTTTCATCACCAATGGCTTTGTGTCGCACGCTGGCGGCCGAGACCAGACCTTGGGATCGCTTATGTGTACAGTTGCAAGGTGTGAGATAGGGATAACAGGGTAATAATAGCTCTTTGGATAAAATCCGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAAGAATTACTGACCCCTCGGGGTCTCA BBF10K_003498_backbone_seq 1 CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAG