LOCUS       BBF10K_003463           2792 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003463
VERSION     BBF10K_003463.1  
FEATURES             Location/Qualifiers
     CDS             5..672
                     /label=T4T5_AmilCP
     CDS             complement(1715..2574)
                     /label=AmpR
     promoter        complement(2576..2679)
                     /label=AmpR promoter
     primer_bind     2698..2714
                     /label=M13 fwd
     primer_bind     complement(853..868)
                     /label=M13 rev
     rep_origin      complement(936..1523)
                     /label=ColE1
     primer_bind     770..788
                     /label=SLiCE-for
     primer_bind     complement(2742..2759)
                     /label=SLiCE-rev
     misc_feature    1527..1716
                     /label=spacer
     CDS             887..937
                     /label=spacer
     terminator      803..850
                     /label=T7 terminator
     terminator      2715..2746
                     /label=tonB terminator
     misc_feature    765..2765
                     /label=pOpen_v3 backbone
     misc_feature    2786..2791
                     /label=BsaI_site
     misc_feature    complement(678..683)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=TTCG
     misc_feature    673..676
                     /label=CGGC
ORIGIN
        1 TTCGGGTGGC TCGGTAATTG CGAAACAAAT GACATATAAA GTTTATATGT CAGGTACGGT
       61 TAATGGTCAT TACTTCGAAG TTGAAGGTGA CGGTAAGGGT AAACCATACG AGGGTGAACA
      121 AACTGTTAAA TTAACAGTAA CAAAAGGTGG CCCTTTACCA TTTGCTTGGG ATATCTTATC
      181 TCCACAATGT CAATACGGTT CAATCCCATT TACTAAGTAT CCAGAAGATA TTCCGGACTA
      241 TGTAAAACAG TCTTTTCCTG AAGGTTACAC TTGGGAACGT ATTATGAACT TCGAGGACGG
      301 TGCAGTATGT ACGGTATCTA ATGATTCTTC TATTCAGGGT AACTGTTTCA TTTATCATGT
      361 AAAATTTTCT GGTTTAAATT TTCCACCAAA CGGGCCAGTA ATGCAAAAAA AAACACAAGG
      421 TTGGGAACCA AATACTGAAC GTTTATTCGC ACGTGATGGT ATGCTTTTAG GTAACAACTT
      481 TATGGCGTTG AAATTAGAGG GCGGTGGTCA TTACTTGTGT GAGTTCAAGA CAACTTATAA
      541 AGCTAAAAAA CCGGTAAAAA TGCCAGGTTA CCACTACGTA GATCGTAAAT TAGATGTTAC
      601 GAACCATAAC AAAGACTATA CTTCTGTAGA ACAATGCGAA ATTAGTATTG CTCGCAAACC
      661 AGTAGTTGCC GGCGGCCGAG ACCACGTGAA TCTTGACCTG TGGTTGCTTC CGAGATCAGG
      721 ACTCGTAGGG ATAACAGGGT AATGAAGTAC ACTAAGTGGT ACAACGCTGA GGTGTCAATC
      781 GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG
      841 GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC
      901 AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA
      961 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
     1021 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
     1081 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
     1141 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
     1201 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
     1261 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
     1321 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC
     1381 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
     1441 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
     1501 TACGCGCAGA AAAAAAGGAT CTCAAGAAGG CCTACTATTA GCAACAACGA TCCTTTGATC
     1561 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG
     1621 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA
     1681 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA
     1741 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG
     1801 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA
     1861 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC
     1921 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT
     1981 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC
     2041 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG
     2101 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC
     2161 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT
     2221 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG
     2281 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT
     2341 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG
     2401 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA
     2461 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA
     2521 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC
     2581 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
     2641 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA
     2701 AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTT CAGGTGGAGT
     2761 GGGAGAAGAA TTACTGACCC CTCGGGGTCT CA
//