LOCUS       BBF10K_003465           2422 bp    DNA     circular     17-Jun-2025
ACCESSION   BBF10K_003465
VERSION     BBF10K_003465.1  
FEATURES             Location/Qualifiers
     CDS             5..42
                     /label=T4T5_GS-flexible
     CDS             complement(1215..2074)
                     /label=AmpR
     promoter        complement(2076..2179)
                     /label=AmpR promoter
     primer_bind     2198..2214
                     /label=M13 fwd
     primer_bind     complement(353..368)
                     /label=M13 rev
     rep_origin      complement(436..1023)
                     /label=ColE1
     primer_bind     270..288
                     /label=SLiCE-for
     primer_bind     complement(2242..2259)
                     /label=SLiCE-rev
     misc_feature    1027..1216
                     /label=spacer
     CDS             387..437
                     /label=spacer
     terminator      303..350
                     /label=T7 terminator
     terminator      2215..2246
                     /label=tonB terminator
     misc_feature    265..2265
                     /label=pOpen_v3 backbone
     misc_feature    2416..2421
                     /label=BsaI_site
     misc_feature    complement(48..53)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=TTCG
     misc_feature    43..46
                     /label=CGGC
ORIGIN
        1 TTCGGGAGGT GGCTCTGGCG GTGGATCAGG TGGAGGCTCT GGCGGCCGAG ACCTTAGAAC
       61 TGGACGTGCG GTTTCTCTGC GAAAAATACC TCGAGCTGTT GCGTTATTGC GCTGCTTAGA
      121 TGCAGTGTTG CACATATCAC TTTTGTTTCA ACGACTGCCG CTTTCGCTGT ATCCTTAGAC
      181 AGTACTATCG GTGCATCATC TCGTTAGATG GGAACCGCCC AGACGTAGGG ATAACAGGGT
      241 AATAGTTGTA AGCGAGGATC AAACCGCTGA GGTGTCAATC GTCGGAGCCG CTGAGCAATA
      301 ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC ATGGTCATAG
      361 CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC AAATTTCGTG AGGAGTCTCC
      421 AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC
      481 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG
      541 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT
      601 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG
      661 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT
      721 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC
      781 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT
      841 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC
      901 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA
      961 CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT
     1021 CTCAAGAAGG CCTACTATTA GCAACAACGA TCCTTTGATC TTTTCTACGG GGTCTGACGC
     1081 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT
     1141 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA
     1201 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT
     1261 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG
     1321 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA CCACGCTCAC CGGCTCCAGA
     1381 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT
     1441 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT
     1501 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT
     1561 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT
     1621 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC
     1681 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC
     1741 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT
     1801 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG
     1861 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT
     1921 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC
     1981 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA
     2041 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG
     2101 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA
     2161 TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA AACGACGGCC AGTAGTCAAA
     2221 AGCCTCCGAC CGGAGGCTTT TGACTTGGTT CAGGTGGAGT GGGAGAAGAA TTACTGACCC
     2281 CTCGGATTAA TTGCCACTGC CAAAATTCTG TCTACAAATG TTTTAGTTCG CTCCAGTAAA
     2341 GTTGTTTATA ACGACTACTA AATCCGCATG TTACGGGATT TCTTATTAAT TCTTTTTTCG
     2401 TAAGGAACAG CGGATGGTCT CA
//