LOCUS       BBF10K_003467           2417 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003467
VERSION     BBF10K_003467.1  
FEATURES             Location/Qualifiers
     CDS             5..31
                     /label=T5E_6H
     CDS             complement(1200..2059)
                     /label=AmpR
     promoter        complement(2061..2164)
                     /label=AmpR promoter
     primer_bind     2183..2199
                     /label=M13 fwd
     primer_bind     complement(338..353)
                     /label=M13 rev
     rep_origin      complement(421..1008)
                     /label=ColE1
     primer_bind     255..273
                     /label=SLiCE-for
     primer_bind     complement(2227..2244)
                     /label=SLiCE-rev
     misc_feature    1012..1201
                     /label=spacer
     CDS             372..422
                     /label=spacer
     terminator      288..335
                     /label=T7 terminator
     terminator      2200..2231
                     /label=tonB terminator
     misc_feature    250..2250
                     /label=pOpen_v3 backbone
     misc_feature    2411..2416
                     /label=BsaI_site
     misc_feature    complement(37..42)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=CGGC
     misc_feature    32..35
                     /label=GCTT
ORIGIN
        1 CGGCCATCAC CATCACCATC ACTAATGATA GGCTTCGAGA CCATTACCTA CAACGGTCGA
       61 CCATACCTTC GATTATCGTG GCCACTCTCG GATTACACGG CAGAGGTGGT TGTGTTGCGA
      121 TAATTTAGAC CCTAATATCA CATCATTAGA CATTAATTGC CTCTGCCAAG GTTACAATAA
      181 GGGCTCCGTA GGAAATTGTA GAAGCAAATA TAGGGATAAC AGGGTAATGA CTGAATCGAT
      241 ATAAATGAGC GCTGAGGTGT CAATCGTCGG AGCCGCTGAG CAATAACTAG CATAACCCCT
      301 TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT CATAGCTGTT TCCTGAGAGC
      361 TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG TCTCCAGAAG AATGCCATTA
      421 ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT
      481 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC
      541 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA
      601 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT
      661 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA
      721 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG
      781 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC
      841 CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA
      901 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG
      961 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGGCCTAC
     1021 TATTAGCAAC AACGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA
     1081 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT
     1141 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG
     1201 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT
     1261 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC
     1321 CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA
     1381 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA
     1441 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA
     1501 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT
     1561 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC
     1621 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT
     1681 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC
     1741 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG
     1801 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT
     1861 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC
     1921 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG
     1981 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC
     2041 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG
     2101 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT
     2161 TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG
     2221 GCTTTTGACT TGGTTCAGGT GGAGTGGGAG AAGAATTACT GACCCCTCGG TCGGGTCATA
     2281 CTATAAGATT CCACAATGCG ACTACTTATA AGATGTCTTA ACGGTATCCG GGCCAGTATA
     2341 TTATTCTAAG GCGTTACCCT AATGAATTTT CATCAGAATT GCTATAGCCC TTGAACGCTA
     2401 CATGCACGAT GGTCTCA
//