LOCUS BBF10K_003469 3705 bp DNA circular 29-Apr-2025 ACCESSION BBF10K_003469 VERSION BBF10K_003469.1 FEATURES Location/Qualifiers CDS 5..1585 /label=T5E_CBD CDS complement(2628..3487) /label=AmpR promoter complement(3489..3592) /label=AmpR promoter primer_bind 3611..3627 /label=M13 fwd primer_bind complement(1766..1781) /label=M13 rev rep_origin complement(1849..2436) /label=ColE1 primer_bind 1683..1701 /label=SLiCE-for primer_bind complement(3655..3672) /label=SLiCE-rev misc_feature 2440..2629 /label=spacer CDS 1800..1850 /label=spacer terminator 1716..1763 /label=T7 terminator terminator 3628..3659 /label=tonB terminator misc_feature 1678..3678 /label=pOpen_v3 backbone misc_feature 3699..3704 /label=BsaI_site misc_feature complement(1591..1596) /label=BsaI_site misc_feature 1..4 /label=CGGC misc_feature 1586..1589 /label=GCTT ORIGIN 1 CGGCAAGATC GAAGAAGGGA AGTTAGTGAT CGGATCGTTA GAAGGCTGCT TTGCGAAAGG 61 AACCAATGTC CTGATGGCGG ACGGTAGCAT TGAATGTATC GAAAACATCG AAGTCGGAAA 121 CAAGGTGATG GGGAAAGATG GTCGTCCGCG TGAAGTTATC AAACTGCCCC GTGGACGCGA 181 AACTATGTAC TCAGTTGTCC AAAAGTCCCA GCACCGTGCG CACAAAAGTG ATTCGTCGCG 241 TGAAGTGCCC GAGCTGTTGA AGTTCACCTG TAACGCAACG CACGAACTGG TAGTGCGTAC 301 ACCTCGCTCT GTGCGTCGTC TGTCTCGTAC CATTAAGGGT GTCGAGTATT TCGAGGTGAT 361 CACTTTCGAA ATGGGTCAGA AGAAGGCACC CGACGGTCGT ATTGTGGAAC TGGTGAAAGA 421 GGTGTCAAAG TCATACCCGA TCAGCGAAGG TCCGGAACGT GCCAATGAGT TAGTTGAATC 481 GTACCGCAAA GCTTCTAACA AAGCATACTT CGAATGGACC ATCGAAGCGC GTGACCTGTC 541 TCTGCTGGGG AGCCATGTGC GTAAAGCAAC CTATCAGACC TACGCTCCGA TCCTGTACGA 601 GAACGACCAC TTCTTTGATT ACATGCAGAA ATCGAAATTC CACCTGACCA TCGAGGGGCC 661 AAAAGTCCTG GCCTACTTAC TGGGTCTTTG GATTGGTGAT GGTCTTAGCG ATCGTGCAAC 721 CTTCTCAGTA GATAGTCGTG ATACCTCTCT GATGGAACGT GTGACTGAGT ATGCGGAAAA 781 GCTGAATCTG TGTGCCGAGT ACAAGGACCG TAAGGAACCG CAAGTCGCGA AAACCGTTAA 841 CCTGTATTCA AAAGTTGTTC GTGGTGCATC TACGAACCCG GGAGTTAGTG CGTGGCAAGT 901 CAACACCGCT TATACGGCTG GCCAACTGGT AACCTATAAC GGGAAAACGT ACAAGTGCCT 961 GCAACCGCAC ACCTCCTTGG CGGGATGGGA GCCGAGCAAC GTGCCAGCGC TGTGGCAATT 1021 GCAGGGCGGT CACGGCGGTA TCCGCAACAA TTTGAATACG GAAAACCCCT TGTGGGACGC 1081 GATCGTGGGC CTGGGTTTCC TGAAAGACGG GGTCAAGAAC ATCCCATCCT TCCTGAGCAC 1141 CGATAACATT GGTACGCGTG AGACATTCCT GGCAGGATTG ATTGATTCGG ATGGCTATGT 1201 GACTGACGAA CACGGTATTA AGGCTACTAT CAAAACCATT CATACCTCTG TTCGCGATGG 1261 GCTGGTTTCT TTAGCTCGCT CGCTGGGTTT AGTGGTGAGC GTTAACGCGG AACCGGCAAA 1321 AGTCGATATG AATGTGACCA AACACAAGAT CTCTTATGCG ATTTACATGT CTGGCGGAGA 1381 TGTTCTGCTT AACGTTCTGT CTAAATGTGC GGGTAGCAAG AAATTCCGTC CTGCGCCAGC 1441 GGCAGCTTTT GCGCGTGAAT GTCGTGGTTT CTACTTCGAA TTGCAAGAAT TGAAAGAGGA 1501 CGACTATTAC GGCATTACCC TGTCCGACGA CAGCGATCAT CAATTCCTGT TGGGTTCTCA 1561 AGTGGTCGTA CAAAACTAAT GATAGGCTTC GAGACCAGGC TCGATACAGT TTCACGTTAG 1621 ACAATCGGAA ATGATTTGTA GGGATAACAG GGTAATGAAA AGATTCCTCA GTGGACTCGC 1681 TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA 1741 ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT 1801 GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC 1861 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA 1921 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC 1981 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 2041 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT 2101 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG 2161 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA 2221 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT 2281 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 2341 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT 2401 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGGCCTACTA TTAGCAACAA 2461 CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 2521 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 2581 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT 2641 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT 2701 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT 2761 GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG 2821 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG 2881 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT 2941 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC 3001 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT 3061 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC 3121 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA 3181 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC 3241 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA 3301 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA 3361 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG 3421 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG 3481 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT 3541 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG 3601 CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG 3661 GTTCAGGTGG AGTGGGAGAA GAATTACTGA CCCCTCGGGG TCTCA //