LOCUS       BBF10K_003470           2451 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003470
VERSION     BBF10K_003470.1  
FEATURES             Location/Qualifiers
     CDS             5..331
                     /label=T5E_Cex
     CDS             complement(1374..2233)
                     /label=AmpR
     promoter        complement(2235..2338)
                     /label=AmpR promoter
     primer_bind     2357..2373
                     /label=M13 fwd
     primer_bind     complement(512..527)
                     /label=M13 rev
     rep_origin      complement(595..1182)
                     /label=ColE1
     primer_bind     429..447
                     /label=SLiCE-for
     primer_bind     complement(2401..2418)
                     /label=SLiCE-rev
     misc_feature    1186..1375
                     /label=spacer
     CDS             546..596
                     /label=spacer
     terminator      462..509
                     /label=T7 terminator
     terminator      2374..2405
                     /label=tonB terminator
     misc_feature    424..2424
                     /label=pOpen_v3 backbone
     misc_feature    2445..2450
                     /label=BsaI_site
     misc_feature    complement(337..342)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=CGGC
     misc_feature    332..335
                     /label=GCTT
ORIGIN
        1 CGGCCCTGCA GGTTGTCAGG TGCTGTGGGG CGTCAACCAG TGGAATACTG GCTTTACCGC
       61 AAACGTCACG GTGAAAAACA CCTCGTCTGC TCCGGTTGAT GGCTGGACAT TAACTTTCTC
      121 GTTTCCGAGC GGTCAGCAGG TGACGCAGGC GTGGTCCAGT ACCGTTACGC AATCCGGAAG
      181 CGCGGTAACC GTACGCAACG CCCCGTGGAA TGGCAGTATT CCAGCTGGGG GCACTGCGCA
      241 ATTTGGTTTC AATGGTAGCC ATACCGGTAC CAATGCGGCC CCCACAGCCT TCTCACTGAA
      301 CGGGACCCCG TGCACGGTGG GATAATGATA GGCTTCGAGA CCAGCCATTG GAAAACTGAA
      361 CGTTATTCGT TTTGCCAATT TCTATAGGGA TAACAGGGTA ATATAAACTG GCACGTGCAA
      421 CCGCGCTGAG GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC
      481 CTCTAAACGG GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA
      541 GGTGATGACA CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC
      601 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA
      661 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC
      721 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG
      781 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC
      841 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC
      901 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA
      961 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT
     1021 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG
     1081 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
     1141 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGGC CTACTATTAG
     1201 CAACAACGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
     1261 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA
     1321 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA
     1381 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC
     1441 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC
     1501 TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC
     1561 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT
     1621 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT
     1681 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC
     1741 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG
     1801 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT
     1861 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC
     1921 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG
     1981 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT
     2041 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC
     2101 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC
     2161 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA
     2221 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG
     2281 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG
     2341 CACCTGCACC AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT
     2401 GACTTGGTTC AGGTGGAGTG GGAGAAGAAT TACTGACCCC TCGGGGTCTC A
//