LOCUS       BBF10K_003471           3231 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003471
VERSION     BBF10K_003471.1  
FEATURES             Location/Qualifiers
     CDS             5..1111
                     /label=T5E_MBP
     CDS             complement(2154..3013)
                     /label=AmpR
     promoter        complement(3015..3118)
                     /label=AmpR promoter
     primer_bind     3137..3153
                     /label=M13 fwd
     primer_bind     complement(1292..1307)
                     /label=M13 rev
     rep_origin      complement(1375..1962)
                     /label=ColE1
     primer_bind     1209..1227
                     /label=SLiCE-for
     primer_bind     complement(3181..3198)
                     /label=SLiCE-rev
     misc_feature    1966..2155
                     /label=spacer
     CDS             1326..1376
                     /label=spacer
     terminator      1242..1289
                     /label=T7 terminator
     terminator      3154..3185
                     /label=tonB terminator
     misc_feature    1204..3204
                     /label=pOpen_v3 backbone
     misc_feature    3225..3230
                     /label=BsaI_site
     misc_feature    complement(1117..1122)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=CGGC
     misc_feature    1112..1115
                     /label=GCTT
ORIGIN
        1 CGGCAAAATC GAAGAAGGTA AACTGGTAAT CTGGATTAAC GGCGATAAAG GCTATAACGG
       61 CCTCGCTGAA GTCGGTAAGA AATTCGAGAA AGATACCGGA ATTAAAGTCA CCGTTGAGCA
      121 TCCGGATAAA CTGGAAGAGA AATTCCCACA GGTTGCGGCA ACTGGCGATG GCCCTGACAT
      181 TATCTTCTGG GCACACGACC GCTTTGGTGG CTACGCTCAA TCTGGCCTGT TGGCTGAAAT
      241 CACCCCGGAC AAAGCGTTCC AGGACAAGCT GTATCCGTTT ACCTGGGATG CCGTACGTTA
      301 CAACGGCAAG CTGATTGCTT ACCCGATCGC TGTTGAAGCG TTATCGCTGA TTTATAACAA
      361 AGACCTGCTG CCGAACCCGC CAAAAACCTG GGAAGAGATC CCGGCGCTGG ATAAAGAACT
      421 GAAAGCGAAA GGTAAGAGCG CGCTGATGTT CAACCTGCAA GAACCGTACT TCACCTGGCC
      481 GCTGATTGCT GCTGACGGGG GTTATGCGTT CAAGTATGAA AACGGCAAGT ACGACATTAA
      541 AGACGTGGGC GTGGATAACG CTGGCGCGAA AGCGGGTCTG ACCTTCCTGG TTGACCTGAT
      601 TAAAAACAAA CACATGAATG CAGACACCGA TTACTCCATC GCAGAAGCTG CCTTTAATAA
      661 AGGCGAAACA GCGATGACCA TCAACGGCCC GTGGGCATGG TCCAACATCG ACACCAGCAA
      721 AGTGAATTAT GGTGTAACGG TACTGCCGAC CTTCAAGGGT CAACCATCCA AACCGTTCGT
      781 TGGCGTGCTG AGCGCAGGTA TTAACGCCGC CAGTCCGAAC AAAGAGCTGG CGAAAGAGTT
      841 CCTCGAAAAC TATCTGCTGA CTGATGAAGG TCTGGAAGCG GTTAATAAAG ACAAACCGCT
      901 GGGTGCCGTA GCGCTGAAGT CTTACGAGGA AGAGTTGGCG AAAGATCCAC GTATTGCCGC
      961 CACGATGGAA AACGCCCAGA AAGGTGAAAT CATGCCGAAC ATCCCGCAGA TGTCCGCTTT
     1021 CTGGTATGCC GTGCGTACTG CGGTGATCAA CGCCGCCAGC GGTCGTCAGA CTGTCGATGA
     1081 AGCCCTGAAA GACGCGCAGA CTTAATGATA GGCTTCGAGA CCAGGGTGCC TTGCTAATCT
     1141 AGTGAAACGT GAAGTTCCGG TTCATAGGGA TAACAGGGTA ATATCCTTTA GGATGGTTCG
     1201 CTGCGCTGAG GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC
     1261 CTCTAAACGG GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA
     1321 GGTGATGACA CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC
     1381 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA
     1441 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC
     1501 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG
     1561 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC
     1621 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC
     1681 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA
     1741 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT
     1801 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG
     1861 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
     1921 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGGC CTACTATTAG
     1981 CAACAACGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
     2041 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA
     2101 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA
     2161 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC
     2221 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC
     2281 TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC
     2341 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT
     2401 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT
     2461 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC
     2521 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG
     2581 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT
     2641 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC
     2701 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG
     2761 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT
     2821 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC
     2881 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC
     2941 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA
     3001 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG
     3061 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG
     3121 CACCTGCACC AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT
     3181 GACTTGGTTC AGGTGGAGTG GGAGAAGAAT TACTGACCCC TCGGGGTCTC A
//