LOCUS       BBF10K_003472           2410 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003472
VERSION     BBF10K_003472.1  
FEATURES             Location/Qualifiers
     CDS             5..70
                     /label=T5E_R5
     CDS             complement(1223..2082)
                     /label=AmpR
     promoter        complement(2084..2187)
                     /label=AmpR promoter
     primer_bind     2206..2222
                     /label=M13 fwd
     primer_bind     complement(361..376)
                     /label=M13 rev
     rep_origin      complement(444..1031)
                     /label=ColE1
     primer_bind     278..296
                     /label=SLiCE-for
     primer_bind     complement(2250..2267)
                     /label=SLiCE-rev
     misc_feature    1035..1224
                     /label=spacer
     CDS             395..445
                     /label=spacer
     terminator      311..358
                     /label=T7 terminator
     terminator      2223..2254
                     /label=tonB terminator
     misc_feature    273..2273
                     /label=pOpen_v3 backbone
     misc_feature    2404..2409
                     /label=BsaI_site
     misc_feature    complement(76..81)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=CGGC
     misc_feature    71..74
                     /label=GCTT
ORIGIN
        1 CGGCTCCTCT AAAAAGTCTG GTTCCTACTC TGGTAGCAAA GGCTCCAAAC GTCGCATCCT
       61 GTAATGATAG GCTTCGAGAC CCTAGTTCCT TTACAAAGTA CTGGTTCTCT TTTCGGCAAG
      121 ATGCCTTATC TAAATGCAAT GACGGACGTA TTCCTTTAGC CACATCGGTT CCTGCTTTCG
      181 CTGAAATCCA AAGGCCTTTT ATTGGGTCCA GTCATATTAA CTCCTTACCA CCCTAGGGAT
      241 AACAGGGTAA TTACCGACGT AATCCGTGCC TGCGCTGAGG TGTCAATCGT CGGAGCCGCT
      301 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT
      361 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG
      421 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA
      481 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
      541 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
      601 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
      661 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
      721 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
      781 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
      841 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT
      901 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
      961 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
     1021 AAAAGGATCT CAAGAAGGCC TACTATTAGC AACAACGATC CTTTGATCTT TTCTACGGGG
     1081 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
     1141 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
     1201 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
     1261 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA
     1321 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG
     1381 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT
     1441 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT
     1501 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
     1561 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA
     1621 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
     1681 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC
     1741 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA
     1801 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
     1861 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA
     1921 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT
     1981 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC
     2041 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA
     2101 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
     2161 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG
     2221 TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTTCA GGTGGAGTGG GAGAAGAATT
     2281 ACTGACCCCT CGGCATTGGC AACTGAAACC GCCATTCCAT AGTGAGTCCT TCGTCTGTGA
     2341 CTATCTGTGC CAAATCGTCT AGCAAACTGC TAATCCAGTT TATCTCACCA AATTATAGCC
     2401 ATAGGTCTCA
//