LOCUS       BBF10K_003473           2553 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003473
VERSION     BBF10K_003473.1  
FEATURES             Location/Qualifiers
     CDS             5..433
                     /label=T5E_CipA
     CDS             complement(1476..2335)
                     /label=AmpR
     promoter        complement(2337..2440)
                     /label=AmpR promoter
     primer_bind     2459..2475
                     /label=M13 fwd
     primer_bind     complement(614..629)
                     /label=M13 rev
     rep_origin      complement(697..1284)
                     /label=ColE1
     primer_bind     531..549
                     /label=SLiCE-for
     primer_bind     complement(2503..2520)
                     /label=SLiCE-rev
     misc_feature    1288..1477
                     /label=spacer
     CDS             648..698
                     /label=spacer
     terminator      564..611
                     /label=T7 terminator
     terminator      2476..2507
                     /label=tonB terminator
     misc_feature    526..2526
                     /label=pOpen_v3 backbone
     misc_feature    2547..2552
                     /label=BsaI_site
     misc_feature    complement(439..444)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=CGGC
     misc_feature    434..437
                     /label=GCTT
ORIGIN
        1 CGGCAATAGC ATTAATCCGC AGTTTAAAGT GACCAATACC GGCAGCAGCG CGATTGATCT
       61 GAGCAAACTG ACCCTGCGCT ATTATTATAC CGTGGATGGC CAGAAAGATC AGACCTTTTG
      121 GTGCGATCAT GCGGCGATTA TTGGCAGCAA TGGCAGCTAT AATGGCATTA CCAGCAATGT
      181 GAAAGGCACC TTTGTGAAAA TGAGCAGCAG CACCAATAAT GCGGATACCT ATCTGGAAAT
      241 TAGCTTTACC GGCGGCACCC TGGAACCGGG CGCGCATGTG CAGATTCAGG GCCGCTTTGC
      301 GAAAAATGAT TGGAGCAATT ATACCCAGAG CAATGATTAT AGCTTTAAAA GCGCGAGCCA
      361 GTTTGTGGAA TGGGATCAGG TGACCGCGTA TCTGAATGGC GTGCTGGTGT GGGGCAAAGA
      421 ACCGTAATGA TAGGCTTCGA GACCACAGTC CTTGCCAATC GAAGTACATA TCATGACTCT
      481 AACTTGTAGG GATAACAGGG TAATCGTGAG TGTCGATCGG ATGGGCGCTG AGGTGTCAAT
      541 CGTCGGAGCC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG
      601 GGGTTTTTTG CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA
      661 CAAATTTCGT GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG
      721 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA
      781 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC
      841 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC
      901 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC
      961 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG
     1021 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT
     1081 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA
     1141 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT
     1201 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA
     1261 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG GCCTACTATT AGCAACAACG ATCCTTTGAT
     1321 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT
     1381 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC
     1441 AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC
     1501 ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA
     1561 GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA
     1621 ACCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG
     1681 CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC
     1741 TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT
     1801 CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG
     1861 GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT
     1921 CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA
     1981 TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA
     2041 GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA
     2101 TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG
     2161 GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC
     2221 ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG
     2281 AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT
     2341 CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT
     2401 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA
     2461 AAACGACGGC CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT TCAGGTGGAG
     2521 TGGGAGAAGA ATTACTGACC CCTCGGGGTC TCA
//