LOCUS       BBF10K_003474           2413 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003474
VERSION     BBF10K_003474.1  
FEATURES             Location/Qualifiers
     CDS             5..13
                     /label=T5E_3xStop
     CDS             complement(1196..2055)
                     /label=AmpR
     promoter        complement(2057..2160)
                     /label=AmpR promoter
     primer_bind     2179..2195
                     /label=M13 fwd
     primer_bind     complement(334..349)
                     /label=M13 rev
     rep_origin      complement(417..1004)
                     /label=ColE1
     primer_bind     251..269
                     /label=SLiCE-for
     primer_bind     complement(2223..2240)
                     /label=SLiCE-rev
     misc_feature    1008..1197
                     /label=spacer
     CDS             368..418
                     /label=spacer
     terminator      284..331
                     /label=T7 terminator
     terminator      2196..2227
                     /label=tonB terminator
     misc_feature    246..2246
                     /label=pOpen_v3 backbone
     misc_feature    2407..2412
                     /label=BsaI_site
     misc_feature    complement(19..24)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=CGGC
     misc_feature    14..17
                     /label=GCTT
ORIGIN
        1 CGGCTAATGA TAGGCTTCGA GACCTGCTTG TGTCCCAACA GGCTAGGATA TAATGCTGAA
       61 GCCTTTCCCC AAACGTTCAG GGTGGGATTT GCTACAACTT CTGAGTCCAA CATGTTCGTA
      121 TTCATGTTAT ATATGCACAA GGCCGAGAAT TGGACGTAGC TTTCACCCCG TGAATGCTAT
      181 GTTCTCCTTG GCCGATCCCA AAGCGTTAGG GATAACAGGG TAATCATCTT ATGATTTTGG
      241 CGTAGCGCTG AGGTGTCAAT CGTCGGAGCC GCTGAGCAAT AACTAGCATA ACCCCTTGGG
      301 GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CATGGTCATA GCTGTTTCCT GAGAGCTTGG
      361 CAGGTGATGA CACACATTAA CAAATTTCGT GAGGAGTCTC CAGAAGAATG CCATTAATTT
      421 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
      481 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
      541 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
      601 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
      661 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
      721 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
      781 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
      841 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
      901 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
      961 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG GCCTACTATT
     1021 AGCAACAACG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
     1081 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
     1141 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC
     1201 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
     1261 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT
     1321 GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG
     1381 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
     1441 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT
     1501 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC
     1561 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT
     1621 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG
     1681 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
     1741 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT
     1801 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC
     1861 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT
     1921 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT
     1981 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
     2041 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT
     2101 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG
     2161 CGCACCTGCA CCAGTCAGTA AAACGACGGC CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT
     2221 TTGACTTGGT TCAGGTGGAG TGGGAGAAGA ATTACTGACC CCTCGGCCGG ACTAAAGTGG
     2281 CGAAAATTAT GGTGTGTGAC CCGTTATGCT CCAGTTCGGT CAGTGGGTCA TTGCAAGTAG
     2341 TCGATTGCAT TGTCAATCTC CGAGTGATTT AGCGTGACAG CCGCAGGGAA CCCATAAAAT
     2401 GTGATCGGTC TCA
//