LOCUS BBF10K_003475 2414 bp DNA circular 29-Apr-2025 ACCESSION BBF10K_003475 VERSION BBF10K_003475.1 FEATURES Location/Qualifiers terminator 5..94 /label=EF_ECK9600 CDS complement(1237..2096) /label=AmpR promoter complement(2098..2201) /label=AmpR promoter primer_bind 2220..2236 /label=M13 fwd primer_bind complement(375..390) /label=M13 rev rep_origin complement(458..1045) /label=ColE1 primer_bind 292..310 /label=SLiCE-for primer_bind complement(2264..2281) /label=SLiCE-rev misc_feature 1049..1238 /label=spacer CDS 409..459 /label=spacer terminator 325..372 /label=T7 terminator terminator 2237..2268 /label=tonB terminator misc_feature 287..2287 /label=pOpen_v3 backbone misc_feature 2408..2413 /label=BsaI_site misc_feature complement(100..105) /label=BsaI_site misc_feature 1..4 /label=GCTT misc_feature 95..98 /label=CGCT ORIGIN 1 GCTTTTCAGC CAAAAAACTT AAGACCGCCG GTCTTGTCCA CTACCTTGCA GTAATGCGGT 61 GGACAGGATC GGCGGTTTTC TTTTCTCTTC TCAACGCTCG AGACCTGCTA GCGTACAGGG 121 TACTCTTACT ATCCATTTGG GTCACAGGAC ACTCGCTGTT TTCGAATTTA CCCTTTATGC 181 GCCGGTATTG AACCACGCTT ATGCCACCAG CCTCAGATTG TTGTCTCAAG TGAATAGTCT 241 ATTAATATAG GGATAACAGG GTAATAAGCT CTGCTCTTGC TAGGAGCGCT GAGGTGTCAA 301 TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA 361 GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG ACACACATTA 421 ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT CCGCCCCCCT 481 GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA 541 AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG 601 CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA 661 CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA 721 CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG 781 GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG 841 TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA 901 ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC 961 TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG 1021 ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC GATCCTTTGA 1081 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA 1141 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT 1201 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG 1261 CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT 1321 AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG 1381 AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC 1441 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG 1501 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA 1561 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA 1621 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA 1681 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA 1741 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA 1801 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG 1861 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG 1921 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG 1981 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG 2041 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC 2101 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA 2161 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC ACCAGTCAGT 2221 AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG TTCAGGTGGA 2281 GTGGGAGAAG AATTACTGAC CCCTCGGCAA CATCGTTACA ACCAGACTGA TACTAGATAT 2341 ATAATGTCCG CCATACAGAC GAAACTAGTT GGAGATTATC GAGCATTCTA TCACGTCGGC 2401 GACCACTGGT CTCA //