LOCUS       BBF10K_003476           2405 bp    DNA     circular     17-Jun-2025
ACCESSION   BBF10K_003476
VERSION     BBF10K_003476.1  
FEATURES             Location/Qualifiers
     terminator      23..70
                     /label=T7 terminator
     terminator      5..69
                     /label=EF_T7term
     CDS             complement(1221..2080)
                     /label=AmpR
     promoter        complement(2082..2185)
                     /label=AmpR promoter
     primer_bind     2204..2220
                     /label=M13 fwd
     primer_bind     complement(359..374)
                     /label=M13 rev
     rep_origin      complement(442..1029)
                     /label=ColE1
     primer_bind     276..294
                     /label=SLiCE-for
     primer_bind     complement(2248..2265)
                     /label=SLiCE-rev
     misc_feature    1033..1222
                     /label=spacer
     CDS             393..443
                     /label=spacer
     terminator      309..356
                     /label=T7 terminator
     terminator      2221..2252
                     /label=tonB terminator
     misc_feature    271..2271
                     /label=pOpen_v3 backbone
     misc_feature    2399..2404
                     /label=BsaI_site
     misc_feature    complement(75..80)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=GCTT
     misc_feature    70..73
                     /label=CGCT
ORIGIN
        1 GCTTGGTGCT TAATAAGGAT CCTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG
       61 GGTTTTTTGC GCTCGAGACC ATTTATTAGA CCGACCTACA TTCGGCATTG TGGGCAAAAT
      121 GAAGTATTGG CAAACATTAA GTGTCGAACT AGATCTGACC TAACGGTAAG AGAGTTTCAT
      181 AATACGTCCA CCTACGGTTA AGGCTTGTCT AGGCAAAGGG GAATGTTGCT TTAGGGATAA
      241 CAGGGTAATG TTCATAAAAC TCACTCTCAA CGCTGAGGTG TCAATCGTCG GAGCCGCTGA
      301 GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG
      361 TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA
      421 GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA
      481 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC
      541 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT
      601 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA
      661 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG
      721 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT
      781 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA
      841 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT
      901 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC
      961 AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA
     1021 AAGGATCTCA AGAAGGCCTA CTATTAGCAA CAACGATCCT TTGATCTTTT CTACGGGGTC
     1081 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG
     1141 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA
     1201 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT
     1261 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG
     1321 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC
     1381 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC
     1441 AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC
     1501 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC
     1561 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC
     1621 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA
     1681 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT
     1741 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA
     1801 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA
     1861 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG
     1921 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC
     1981 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC
     2041 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA
     2101 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA
     2161 GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA
     2221 GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTTCAGG TGGAGTGGGA GAAGAATTAC
     2281 TGACCCCTCG GGTATCGAAA GTACAAGACT AGCCTTGCTA GCAACCGCGG GCTAGGAGCC
     2341 TAAGGTATCA CTCAAGATAC AGGCTCGGTA ACATACGCTC TAGCTATCTA ACTATCCAGG
     2401 TCTCA
//