LOCUS       BBF10K_003477           2426 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003477
VERSION     BBF10K_003477.1  
FEATURES             Location/Qualifiers
     terminator      253..300
                     /label=T7 terminator
     terminator      5..306
                     /label=EF_TZ
     CDS             complement(1349..2208)
                     /label=AmpR
     promoter        complement(2210..2313)
                     /label=AmpR promoter
     primer_bind     2332..2348
                     /label=M13 fwd
     primer_bind     complement(487..502)
                     /label=M13 rev
     rep_origin      complement(570..1157)
                     /label=ColE1
     primer_bind     404..422
                     /label=SLiCE-for
     primer_bind     complement(2376..2393)
                     /label=SLiCE-rev
     misc_feature    1161..1350
                     /label=spacer
     CDS             521..571
                     /label=spacer
     terminator      437..484
                     /label=T7 terminator
     terminator      2349..2380
                     /label=tonB terminator
     misc_feature    399..2399
                     /label=pOpen_v3 backbone
     misc_feature    2420..2425
                     /label=BsaI_site
     misc_feature    complement(312..317)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=GCTT
     misc_feature    307..310
                     /label=CGCT
ORIGIN
        1 GCTTGGATCC GAATTCGAGC TCCGTCGACC TAGCATAACC CCGCCGGGCC TCTTCGGGGG
       61 TCTGGCGGGG TTTTTTGCTG AAAGAAGCTT CAAATAAAAC GAAAGGCTCA GTCGAAAGAC
      121 TGGGCCTTTC GTTTTATCTG TTGTTTGTCG CTGCGGCCGC ACTCGAGCAC CACCACCACC
      181 ACCACTGAGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG
      241 CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC
      301 TGAAAGCGCT CGAGACCACT GGAATCAGCC TTGTGTCTTT TAGTACATTA GTCTAGCGTT
      361 AGGGATAACA GGGTAATTTC GCCTCCGTGC TAGCATACCG CTGAGGTGTC AATCGTCGGA
      421 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT
      481 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT
      541 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA
      601 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
      661 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
      721 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
      781 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
      841 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
      901 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
      961 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT
     1021 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
     1081 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
     1141 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT
     1201 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
     1261 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
     1321 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC
     1381 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT
     1441 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC
     1501 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT
     1561 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA
     1621 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG
     1681 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT
     1741 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC
     1801 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT
     1861 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC
     1921 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC
     1981 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA
     2041 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC
     2101 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA
     2161 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT
     2221 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
     2281 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC
     2341 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTTCAGGTG GAGTGGGAGA
     2401 AGAATTACTG ACCCCTCGGG GTCTCA
//