Biofoundry-assisted expression and characterization of plant proteins
type: Collection
persistent identity: CellFreeExpression collection
display id: CellFreeExpression_collection
version: 1
description: Many goals in synthetic biology, including the elucidation and refactoring of biosynthetic pathways and the engineering of regulatory circuits and networks, require knowledge of protein function. In plants, the prevalence of large gene families means it can be particularly challenging to link specific functions to individual proteins. However, protein characterization has remained a technical bottleneck, often requiring significant effort to optimize expression and purification protocols. To leverage the ability of biofoundries to accelerate design–built–test–learn cycles, we present a workflow for automated DNA assembly and cell-free expression of plant proteins that accelerates optimization and enables rapid screening of enzyme activity. First, we developed a phytobrick-compatible Golden Gate DNA assembly toolbox containing plasmid acceptors for cell-free expression using Escherichiacoli or wheat germ lysates as well as a set of N- and C-terminal tag parts for detection, purification and improved expression/folding. We next optimized automated assembly of miniaturized cell-free reactions using an acoustic liquid handling platform and then compared tag configurations to identify those that increase expression. We additionally developed a luciferase-based system for rapid quantification that requires a minimal 11–amino acid tag and demonstrate facile removal of tags following synthesis. Finally, we show that several functional assays can be performed with cell-free protein synthesis reactions without the need for protein purification. Together, the combination of automated assembly of DNA parts and cell-free expression reactions should significantly increase the throughput of experiments to test and understand plant protein function and enable the direct reuse of DNA parts in downstream plant engineering workflows.
OBI_0001617: 34693026
creator: Connor Tansley
created: 2024-10-15T15:49:45.756Z
owned by: ct677
owned by: fxbuson
owned by: ykh26
member: pEPQD0KN0024 backbone
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Linearized Backbones
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OBI_0001617: 34693026
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OBI_0001617: 34693026
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type: DnaRegion
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sequence annotation: RBS
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sequence annotation: folA/apaH terminator
sequence annotation: pUC origin of replication (derivative of pBR322/pMB1/ColE1)
sequence annotation: backbone from pJL1-sfGFP (Addgene #69496)
sequence annotation: pBR322 (from pUC18)
sequence annotation: T7 terminator
sequence annotation: diadenosine tetraphosphatase (apaH) C-terminal fragment ( aa 198-280) AVI54590.1 from E coli MG1655
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E01 Translational Enhancer
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OBI_0001617: 34693026
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RBS
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{"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
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folA/apaH terminator
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OBI_0001617: 34693026
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pUC origin of replication (derivative of pBR322/pMB1/ColE1)
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OBI_0001617: 34693026
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backbone from pJL1-sfGFP (Addgene #69496)
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pBR322 (from pUC18)
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OBI_0001617: 34693026
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T7 terminator
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OBI_0001617: 34693026
owned by: ct677
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diadenosine tetraphosphatase (apaH) C-terminal fragment ( aa 198-280) AVI54590.1 from E coli MG1655
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display id: annotation5
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
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type: Range
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display id: annotation5_range
version: 1
OBI_0001617: 34693026
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pEPQD0KN0282 backbone
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display id: pEPQD0KN0282_backbone
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: T7 terminator
sequence annotation: pUC origin of replication (derivative of pBR322/pMB1/ColE1)
sequence annotation: E01 Translational Enhancer
sequence annotation: {"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
sequence annotation: RBS
sequence annotation: pBR322 (from pUC18)
sequence annotation: backbone from pJL1-sfGFP (Addgene #69496)
sequence: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282_backbone_seq/1

T7 terminator
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persistent identity: annotation3
display id: annotation3
version: 1
OBI_0001617: 34693026
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pUC origin of replication (derivative of pBR322/pMB1/ColE1)
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OBI_0001617: 34693026
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E01 Translational Enhancer
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OBI_0001617: 34693026
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{"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
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OBI_0001617: 34693026
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RBS
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
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OBI_0001617: 34693026
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pBR322 (from pUC18)
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OBI_0001617: 34693026
owned by: ct677
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backbone from pJL1-sfGFP (Addgene #69496)
type: SequenceAnnotation
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version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
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pEPQD0KN0283 backbone
type: ComponentDefinition
persistent identity: pEPQD0KN0283 backbone
display id: pEPQD0KN0283_backbone
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: RBS
sequence annotation: {"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
sequence annotation: pBR322 (from pUC18)
sequence annotation: backbone from pJL1-sfGFP (Addgene #69496)
sequence annotation: E01 Translational Enhancer
sequence annotation: T7 terminator
sequence annotation: pUC origin of replication (derivative of pBR322/pMB1/ColE1)
sequence: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283_backbone_seq/1

RBS
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persistent identity: annotation5
display id: annotation5
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
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{"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
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OBI_0001617: 34693026
owned by: ct677
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OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
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pBR322 (from pUC18)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
OBI_0001617: 34693026
owned by: ct677
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persistent identity: annotation6 range
display id: annotation6_range
version: 1
OBI_0001617: 34693026
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backbone from pJL1-sfGFP (Addgene #69496)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
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E01 Translational Enhancer
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0283 backbone
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283_backbone/annotation4/annotation4_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283_backbone/annotation4/annotation4_range/1
type: Range
persistent identity: annotation4 range
display id: annotation4_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0283 backbone
start: 1792
end: 1863
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0283 backbone
role: SO:0000141
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283_backbone/annotation3/annotation3_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283_backbone/annotation3/annotation3_range/1
type: Range
persistent identity: annotation3 range
display id: annotation3_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0283 backbone
start: 65
end: 112
orientation: inline

pUC origin of replication (derivative of pBR322/pMB1/ColE1)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0283 backbone
role: SO:0000296
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283_backbone/annotation1/annotation1_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283_backbone/annotation1/annotation1_range/1
type: Range
persistent identity: annotation1 range
display id: annotation1_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0283 backbone
start: 1067
end: 1655
orientation: inline

pEPQD0KN0285
type: ComponentDefinition
persistent identity: pEPQD0KN0285
display id: pEPQD0KN0285
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: MCS
sequence annotation: folA/apaH terminator
sequence annotation: pBR322 (from pUC18)
sequence annotation: Plac promoter
sequence annotation: E01 Translational Enhancer
sequence annotation: CAP binding site
sequence annotation: diadenosine tetraphosphatase (apaH) C-terminal fragment ( aa 198-280) AVI54590.1 from E coli MG1655
sequence annotation: lac promoter
sequence annotation: lac_promoter
sequence annotation: T7 terminator
sequence annotation: lac operator
sequence annotation: pUC origin of replication (derivative of pBR322/pMB1/ColE1)
sequence annotation: RBS
sequence annotation: {'lacZ-alpha', 'lacZ beta-galactosidase alpha peptide'}
sequence: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285_seq/1

MCS
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0285
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285/annotation2/annotation2_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285/annotation2/annotation2_range/1
type: Range
persistent identity: annotation2 range
display id: annotation2_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0285
start: 1350
end: 1406
orientation: inline

folA/apaH terminator
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0285
role: SO:0000141
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285/annotation10/annotation10_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285/annotation10/annotation10_range/1
type: Range
persistent identity: annotation10 range
display id: annotation10_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0285
start: 1633
end: 1709
orientation: inline

pBR322 (from pUC18)
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0285
role: SO:0000296
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285/annotation13/annotation13_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285/annotation13/annotation13_range/1
type: Range
persistent identity: annotation13 range
display id: annotation13_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0285
start: 166
end: 785
orientation: inline

Plac promoter
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0285
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285/annotation1/annotation1_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285/annotation1/annotation1_range/1
type: Range
persistent identity: annotation1 range
display id: annotation1_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0285
start: 1430
end: 1553
orientation: reverseComplement

E01 Translational Enhancer
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0285
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285/annotation8/annotation8_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285/annotation8/annotation8_range/1
type: Range
persistent identity: annotation8 range
display id: annotation8_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0285
start: 907
end: 978
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0285
role: SO:0000057
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285/annotation5/annotation5_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285/annotation5/annotation5_range/1
type: Range
persistent identity: annotation5 range
display id: annotation5_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0285
start: 1512
end: 1533
orientation: inline

diadenosine tetraphosphatase (apaH) C-terminal fragment ( aa 198-280) AVI54590.1 from E coli MG1655
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0285
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285/annotation9/annotation9_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285/annotation9/annotation9_range/1
type: Range
persistent identity: annotation9 range
display id: annotation9_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0285
start: 1710
end: 1961
orientation: reverseComplement

lac promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0285
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285/annotation4/annotation4_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285/annotation4/annotation4_range/1
type: Range
persistent identity: annotation4 range
display id: annotation4_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0285
start: 1467
end: 1497
orientation: reverseComplement

lac_promoter
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0285
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285/annotation12/annotation12_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285/annotation12/annotation12_range/1
type: Range
persistent identity: annotation12 range
display id: annotation12_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0285
start: 1468
end: 1497
orientation: reverseComplement

T7 terminator
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0285
role: SO:0000141
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285/annotation7/annotation7_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285/annotation7/annotation7_range/1
type: Range
persistent identity: annotation7 range
display id: annotation7_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0285
start: 2309
end: 2356
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0285
role: SO:0000057
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285/annotation3/annotation3_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285/annotation3/annotation3_range/1
type: Range
persistent identity: annotation3 range
display id: annotation3_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0285
start: 1443
end: 1459
orientation: inline

pUC origin of replication (derivative of pBR322/pMB1/ColE1)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0285
role: SO:0000296
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285/annotation6/annotation6_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285/annotation6/annotation6_range/1
type: Range
persistent identity: annotation6 range
display id: annotation6_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0285
start: 182
end: 770
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0285
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285/annotation11/annotation11_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285/annotation11/annotation11_range/1
type: Range
persistent identity: annotation11 range
display id: annotation11_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0285
start: 2465
end: 2471
orientation: inline

{'lacZ-alpha', 'lacZ beta-galactosidase alpha peptide'}
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0285
role: SO:0000316
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285/annotation0/annotation0_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285/annotation0/annotation0_range/1
type: Range
persistent identity: annotation0 range
display id: annotation0_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0285
start: 1100
end: 1423
orientation: reverseComplement

pEPQD0KN0283
type: ComponentDefinition
persistent identity: pEPQD0KN0283
display id: pEPQD0KN0283
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: CAP binding site
sequence annotation: lac promoter
sequence annotation: RBS
sequence annotation: Plac promoter
sequence annotation: lac operator
sequence annotation: MCS
sequence annotation: E01 Translational Enhancer
sequence annotation: {'lacZ-alpha', 'lacZ beta-galactosidase alpha peptide'}
sequence annotation: {"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
sequence annotation: T7 terminator
sequence annotation: lac_promoter
sequence: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283_seq/1

CAP binding site
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0283
role: SO:0000057
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283/annotation6/annotation6_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283/annotation6/annotation6_range/1
type: Range
persistent identity: annotation6 range
display id: annotation6_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0283
start: 743
end: 764
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0283
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283/annotation5/annotation5_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283/annotation5/annotation5_range/1
type: Range
persistent identity: annotation5 range
display id: annotation5_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0283
start: 698
end: 728
orientation: reverseComplement

RBS
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0283
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283/annotation9/annotation9_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283/annotation9/annotation9_range/1
type: Range
persistent identity: annotation9 range
display id: annotation9_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0283
start: 1045
end: 1051
orientation: inline

Plac promoter
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0283
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283/annotation1/annotation1_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283/annotation1/annotation1_range/1
type: Range
persistent identity: annotation1 range
display id: annotation1_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0283
start: 661
end: 784
orientation: reverseComplement

lac operator
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0283
role: SO:0000057
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283/annotation4/annotation4_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283/annotation4/annotation4_range/1
type: Range
persistent identity: annotation4 range
display id: annotation4_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0283
start: 674
end: 690
orientation: inline

MCS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0283
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283/annotation3/annotation3_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283/annotation3/annotation3_range/1
type: Range
persistent identity: annotation3 range
display id: annotation3_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0283
start: 581
end: 637
orientation: inline

E01 Translational Enhancer
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0283
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283/annotation8/annotation8_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283/annotation8/annotation8_range/1
type: Range
persistent identity: annotation8 range
display id: annotation8_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0283
start: 138
end: 209
orientation: inline

{'lacZ-alpha', 'lacZ beta-galactosidase alpha peptide'}
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0283
role: SO:0000316
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283/annotation0/annotation0_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283/annotation0/annotation0_range/1
type: Range
persistent identity: annotation0 range
display id: annotation0_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0283
start: 331
end: 654
orientation: reverseComplement

{"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0283
role: SO:0000316
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283/annotation2/annotation2_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283/annotation2/annotation2_range/1
type: Range
persistent identity: annotation2 range
display id: annotation2_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0283
start: 954
end: 1769
orientation: reverseComplement

T7 terminator
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0283
role: SO:0000141
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283/annotation7/annotation7_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283/annotation7/annotation7_range/1
type: Range
persistent identity: annotation7 range
display id: annotation7_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0283
start: 889
end: 936
orientation: inline

lac_promoter
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0283
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283/annotation10/annotation10_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283/annotation10/annotation10_range/1
type: Range
persistent identity: annotation10 range
display id: annotation10_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0283
start: 699
end: 728
orientation: reverseComplement

pEPQD0CB0026 backbone
type: ComponentDefinition
persistent identity: pEPQD0CB0026 backbone
display id: pEPQD0CB0026_backbone
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: {'tet (887 - 636)', 'tet'}
sequence annotation: CAP binding site
sequence annotation: AmpR_promoter
sequence annotation: lac promoter
sequence annotation: pUC origin of replication (derivative of pBR322/pMB1/ColE1)
sequence annotation: AmpR promoter
sequence annotation: diadenosine tetraphosphatase (apaH) C-terminal fragment ( aa 198-280) AVI54590.1 from E coli MG1655
sequence annotation: pBR322 (from pUC18)
sequence annotation: Plac promoter
sequence annotation: lac operator
sequence annotation: beta-lactamase, ampicillin resistance (ampR)
sequence annotation: lac_promoter
sequence annotation: folA/apaH terminator
sequence annotation: RBS
sequence annotation: E01 Translational Enhancer
sequence: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone_seq/1

{'tet (887 - 636)', 'tet'}
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
role: SO:0000110
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation12/annotation12_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation12/annotation12_range/1
type: Range
persistent identity: annotation12 range
display id: annotation12_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
start: 3083
end: 3333
orientation: reverseComplement

CAP binding site
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
role: SO:0000057
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation3/annotation3_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation3/annotation3_range/1
type: Range
persistent identity: annotation3 range
display id: annotation3_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
start: 756
end: 777
orientation: inline

AmpR_promoter
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation11/annotation11_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation11/annotation11_range/1
type: Range
persistent identity: annotation11 range
display id: annotation11_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
start: 2724
end: 2751
orientation: reverseComplement

lac promoter
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation2/annotation2_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation2/annotation2_range/1
type: Range
persistent identity: annotation2 range
display id: annotation2_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
start: 709
end: 738
orientation: reverseComplement

pUC origin of replication (derivative of pBR322/pMB1/ColE1)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
role: SO:0000296
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation4/annotation4_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation4/annotation4_range/1
type: Range
persistent identity: annotation4 range
display id: annotation4_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
start: 1063
end: 1650
orientation: reverseComplement

AmpR promoter
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation14/annotation14_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation14/annotation14_range/1
type: Range
persistent identity: annotation14 range
display id: annotation14_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
start: 2683
end: 2786
orientation: reverseComplement

diadenosine tetraphosphatase (apaH) C-terminal fragment ( aa 198-280) AVI54590.1 from E coli MG1655
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation6/annotation6_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation6/annotation6_range/1
type: Range
persistent identity: annotation6 range
display id: annotation6_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
start: 115
end: 365
orientation: reverseComplement

pBR322 (from pUC18)
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
role: SO:0000296
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation13/annotation13_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation13/annotation13_range/1
type: Range
persistent identity: annotation13 range
display id: annotation13_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
start: 1048
end: 1666
orientation: reverseComplement

Plac promoter
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation0/annotation0_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation0/annotation0_range/1
type: Range
persistent identity: annotation0 range
display id: annotation0_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
start: 672
end: 794
orientation: reverseComplement

lac operator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
role: SO:0000057
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation1/annotation1_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation1/annotation1_range/1
type: Range
persistent identity: annotation1 range
display id: annotation1_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
start: 687
end: 703
orientation: inline

beta-lactamase, ampicillin resistance (ampR)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
role: SO:0000316
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation10/annotation10_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation10/annotation10_range/1
type: Range
persistent identity: annotation10 range
display id: annotation10_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
start: 1822
end: 2681
orientation: reverseComplement

lac_promoter
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation9/annotation9_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation9/annotation9_range/1
type: Range
persistent identity: annotation9 range
display id: annotation9_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
start: 710
end: 738
orientation: reverseComplement

folA/apaH terminator
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
role: SO:0000141
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation7/annotation7_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation7/annotation7_range/1
type: Range
persistent identity: annotation7 range
display id: annotation7_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
start: 40
end: 116
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation8/annotation8_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation8/annotation8_range/1
type: Range
persistent identity: annotation8 range
display id: annotation8_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
start: 2248
end: 2253
orientation: reverseComplement

E01 Translational Enhancer
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation5/annotation5_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone/annotation5/annotation5_range/1
type: Range
persistent identity: annotation5 range
display id: annotation5_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026 backbone
start: 3595
end: 3666
orientation: inline

pEPQD0KN0284
type: ComponentDefinition
persistent identity: pEPQD0KN0284
display id: pEPQD0KN0284
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: MCS
sequence annotation: folA/apaH terminator
sequence annotation: {"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
sequence annotation: diadenosine tetraphosphatase (apaH) C-terminal fragment ( aa 198-280) AVI54590.1 from E coli MG1655
sequence annotation: lac operator
sequence annotation: Plac promoter
sequence annotation: {'lacZ-alpha', 'lacZ beta-galactosidase alpha peptide'}
sequence annotation: E01 Translational Enhancer
sequence annotation: lac promoter
sequence annotation: T7 terminator
sequence annotation: RBS
sequence annotation: CAP binding site
sequence annotation: lac_promoter
sequence: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284_seq/1

MCS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284/annotation3/annotation3_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284/annotation3/annotation3_range/1
type: Range
persistent identity: annotation3 range
display id: annotation3_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284
start: 581
end: 637
orientation: inline

folA/apaH terminator
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284
role: SO:0000141
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284/annotation10/annotation10_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284/annotation10/annotation10_range/1
type: Range
persistent identity: annotation10 range
display id: annotation10_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284
start: 864
end: 940
orientation: inline

{"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284
role: SO:0000316
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284/annotation2/annotation2_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284/annotation2/annotation2_range/1
type: Range
persistent identity: annotation2 range
display id: annotation2_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284
start: 1602
end: 2417
orientation: reverseComplement

diadenosine tetraphosphatase (apaH) C-terminal fragment ( aa 198-280) AVI54590.1 from E coli MG1655
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284/annotation9/annotation9_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284/annotation9/annotation9_range/1
type: Range
persistent identity: annotation9 range
display id: annotation9_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284
start: 941
end: 1192
orientation: reverseComplement

lac operator
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284
role: SO:0000057
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284/annotation4/annotation4_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284/annotation4/annotation4_range/1
type: Range
persistent identity: annotation4 range
display id: annotation4_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284
start: 674
end: 690
orientation: inline

Plac promoter
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284/annotation1/annotation1_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284/annotation1/annotation1_range/1
type: Range
persistent identity: annotation1 range
display id: annotation1_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284
start: 661
end: 784
orientation: reverseComplement

{'lacZ-alpha', 'lacZ beta-galactosidase alpha peptide'}
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284
role: SO:0000316
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284/annotation0/annotation0_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284/annotation0/annotation0_range/1
type: Range
persistent identity: annotation0 range
display id: annotation0_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284
start: 331
end: 654
orientation: reverseComplement

E01 Translational Enhancer
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284/annotation8/annotation8_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284/annotation8/annotation8_range/1
type: Range
persistent identity: annotation8 range
display id: annotation8_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284
start: 138
end: 209
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284/annotation5/annotation5_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284/annotation5/annotation5_range/1
type: Range
persistent identity: annotation5 range
display id: annotation5_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284
start: 698
end: 728
orientation: reverseComplement

T7 terminator
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284
role: SO:0000141
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284/annotation7/annotation7_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284/annotation7/annotation7_range/1
type: Range
persistent identity: annotation7 range
display id: annotation7_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284
start: 1537
end: 1584
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284/annotation11/annotation11_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284/annotation11/annotation11_range/1
type: Range
persistent identity: annotation11 range
display id: annotation11_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284
start: 1693
end: 1699
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284
role: SO:0000057
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284/annotation6/annotation6_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284/annotation6/annotation6_range/1
type: Range
persistent identity: annotation6 range
display id: annotation6_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284
start: 743
end: 764
orientation: inline

lac_promoter
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284/annotation12/annotation12_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284/annotation12/annotation12_range/1
type: Range
persistent identity: annotation12 range
display id: annotation12_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284
start: 699
end: 728
orientation: reverseComplement

pEPQD0KN0245
type: ComponentDefinition
persistent identity: pEPQD0KN0245
display id: pEPQD0KN0245
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: CAP binding site
sequence annotation: MCS
sequence annotation: lac operator
sequence annotation: RBS
sequence annotation: T7 terminator
sequence annotation: T7 promoter
sequence annotation: {"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
sequence annotation: Plac promoter
sequence annotation: efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)
sequence annotation: pUC origin of replication (derivative of pBR322/pMB1/ColE1)
sequence annotation: lac promoter
sequence annotation: T7 promoter and 5' UTR from pJL1
sequence annotation: {'lacZ-alpha', 'lacZ beta-galactosidase alpha peptide'}
sequence annotation: lac_promoter
sequence: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245_seq/1

CAP binding site
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
role: SO:0000057
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation6/annotation6_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation6/annotation6_range/1
type: Range
persistent identity: annotation6 range
display id: annotation6_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
start: 617
end: 638
orientation: inline

MCS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation3/annotation3_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation3/annotation3_range/1
type: Range
persistent identity: annotation3 range
display id: annotation3_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
start: 455
end: 511
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
role: SO:0000057
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation4/annotation4_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation4/annotation4_range/1
type: Range
persistent identity: annotation4 range
display id: annotation4_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
start: 548
end: 564
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation10/annotation10_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation10/annotation10_range/1
type: Range
persistent identity: annotation10 range
display id: annotation10_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
start: 92
end: 98
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
role: SO:0000141
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation8/annotation8_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation8/annotation8_range/1
type: Range
persistent identity: annotation8 range
display id: annotation8_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
start: 763
end: 810
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation12/annotation12_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation12/annotation12_range/1
type: Range
persistent identity: annotation12 range
display id: annotation12_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
start: 27
end: 45
orientation: inline

{"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
role: SO:0000316
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation2/annotation2_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation2/annotation2_range/1
type: Range
persistent identity: annotation2 range
display id: annotation2_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
start: 828
end: 1643
orientation: reverseComplement

RBS
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation11/annotation11_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation11/annotation11_range/1
type: Range
persistent identity: annotation11 range
display id: annotation11_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
start: 919
end: 925
orientation: inline

Plac promoter
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation1/annotation1_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation1/annotation1_range/1
type: Range
persistent identity: annotation1 range
display id: annotation1_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
start: 535
end: 658
orientation: reverseComplement

efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation9/annotation9_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation9/annotation9_range/1
type: Range
persistent identity: annotation9 range
display id: annotation9_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
start: 77
end: 99
orientation: inline

pUC origin of replication (derivative of pBR322/pMB1/ColE1)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
role: SO:0000296
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation7/annotation7_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation7/annotation7_range/1
type: Range
persistent identity: annotation7 range
display id: annotation7_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
start: 1765
end: 2353
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation5/annotation5_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation5/annotation5_range/1
type: Range
persistent identity: annotation5 range
display id: annotation5_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
start: 572
end: 602
orientation: reverseComplement

T7 promoter and 5' UTR from pJL1
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation13/annotation13_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation13/annotation13_range/1
type: Range
persistent identity: annotation13 range
display id: annotation13_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
start: 27
end: 103
orientation: inline

{'lacZ-alpha', 'lacZ beta-galactosidase alpha peptide'}
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
role: SO:0000316
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation0/annotation0_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation0/annotation0_range/1
type: Range
persistent identity: annotation0 range
display id: annotation0_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
start: 205
end: 528
orientation: reverseComplement

lac_promoter
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation14/annotation14_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245/annotation14/annotation14_range/1
type: Range
persistent identity: annotation14 range
display id: annotation14_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245
start: 573
end: 602
orientation: reverseComplement

pEPQD0KN0025 backbone
type: ComponentDefinition
persistent identity: pEPQD0KN0025 backbone
display id: pEPQD0KN0025_backbone
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: T7 promoter and 5' UTR from pJL1
sequence annotation: efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)
sequence annotation: T7 terminator
sequence annotation: {"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
sequence annotation: pBR322 (from pUC18)
sequence annotation: T7 promoter
sequence annotation: backbone from pJL1-sfGFP (Addgene #69496)
sequence annotation: RBS
sequence annotation: pUC origin of replication (derivative of pBR322/pMB1/ColE1)
sequence: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025_backbone_seq/1

T7 promoter and 5' UTR from pJL1
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025 backbone
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025_backbone/annotation8/annotation8_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025_backbone/annotation8/annotation8_range/1
type: Range
persistent identity: annotation8 range
display id: annotation8_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025 backbone
start: 1683
end: 1759
orientation: inline

efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025 backbone
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025_backbone/annotation4/annotation4_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025_backbone/annotation4/annotation4_range/1
type: Range
persistent identity: annotation4 range
display id: annotation4_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025 backbone
start: 1733
end: 1755
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025 backbone
role: SO:0000141
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025_backbone/annotation3/annotation3_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025_backbone/annotation3/annotation3_range/1
type: Range
persistent identity: annotation3 range
display id: annotation3_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025 backbone
start: 65
end: 112
orientation: inline

{"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025 backbone
role: SO:0000316
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025_backbone/annotation0/annotation0_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025_backbone/annotation0/annotation0_range/1
type: Range
persistent identity: annotation0 range
display id: annotation0_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025 backbone
start: 128
end: 942
orientation: reverseComplement

pBR322 (from pUC18)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025 backbone
role: SO:0000296
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025_backbone/annotation9/annotation9_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025_backbone/annotation9/annotation9_range/1
type: Range
persistent identity: annotation9 range
display id: annotation9_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025 backbone
start: 1051
end: 1670
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025 backbone
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025_backbone/annotation7/annotation7_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025_backbone/annotation7/annotation7_range/1
type: Range
persistent identity: annotation7 range
display id: annotation7_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025 backbone
start: 1683
end: 1701
orientation: inline

backbone from pJL1-sfGFP (Addgene #69496)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025 backbone
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025_backbone/annotation2/annotation2_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025_backbone/annotation2/annotation2_range/1
type: Range
persistent identity: annotation2 range
display id: annotation2_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025 backbone
start: 46
end: 1679
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025 backbone
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025_backbone/annotation5/annotation5_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025_backbone/annotation5/annotation5_range/1
type: Range
persistent identity: annotation5 range
display id: annotation5_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025 backbone
start: 221
end: 227
orientation: inline

pUC origin of replication (derivative of pBR322/pMB1/ColE1)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025 backbone
role: SO:0000296
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025_backbone/annotation1/annotation1_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025_backbone/annotation1/annotation1_range/1
type: Range
persistent identity: annotation1 range
display id: annotation1_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025 backbone
start: 1067
end: 1655
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025 backbone
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025_backbone/annotation6/annotation6_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025_backbone/annotation6/annotation6_range/1
type: Range
persistent identity: annotation6 range
display id: annotation6_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025 backbone
start: 1748
end: 1754
orientation: inline

pEPQD0CB0246
type: ComponentDefinition
persistent identity: pEPQD0CB0246
display id: pEPQD0CB0246
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: lac promoter
sequence annotation: lac operator
sequence annotation: Plac promoter
sequence annotation: {'lacZ-alpha', 'lacZ beta-galactosidase alpha peptide'}
sequence annotation: {'tet (887 - 636)', 'tet'}
sequence annotation: folA/apaH terminator
sequence annotation: CAP binding site
sequence annotation: lac_promoter
sequence annotation: beta-lactamase, ampicillin resistance (ampR)
sequence annotation: AmpR promoter
sequence annotation: diadenosine tetraphosphatase (apaH) C-terminal fragment ( aa 198-280) AVI54590.1 from E coli MG1655
sequence annotation: MCS
sequence annotation: E01 Translational Enhancer
sequence annotation: AmpR_promoter
sequence annotation: RBS
sequence annotation: pUC origin of replication (derivative of pBR322/pMB1/ColE1)
sequence annotation: pBR322 (from pUC18)
sequence: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_seq/1

lac promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation7/annotation7_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation7/annotation7_range/1
type: Range
persistent identity: annotation7 range
display id: annotation7_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
start: 819
end: 849
orientation: reverseComplement

lac operator
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
role: SO:0000057
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation4/annotation4_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation4/annotation4_range/1
type: Range
persistent identity: annotation4 range
display id: annotation4_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
start: 795
end: 811
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
role: SO:0000057
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation5/annotation5_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation5/annotation5_range/1
type: Range
persistent identity: annotation5 range
display id: annotation5_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
start: 1632
end: 1648
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation6/annotation6_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation6/annotation6_range/1
type: Range
persistent identity: annotation6 range
display id: annotation6_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
start: 1656
end: 1686
orientation: reverseComplement

Plac promoter
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation2/annotation2_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation2/annotation2_range/1
type: Range
persistent identity: annotation2 range
display id: annotation2_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
start: 782
end: 905
orientation: reverseComplement

{'lacZ-alpha', 'lacZ beta-galactosidase alpha peptide'}
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
role: SO:0000316
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation0/annotation0_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation0/annotation0_range/1
type: Range
persistent identity: annotation0 range
display id: annotation0_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
start: 452
end: 775
orientation: reverseComplement

{'tet (887 - 636)', 'tet'}
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
role: SO:0000110
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation19/annotation19_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation19/annotation19_range/1
type: Range
persistent identity: annotation19 range
display id: annotation19_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
start: 4030
end: 4281
orientation: reverseComplement

Plac promoter
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation1/annotation1_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation1/annotation1_range/1
type: Range
persistent identity: annotation1 range
display id: annotation1_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
start: 1619
end: 1742
orientation: reverseComplement

folA/apaH terminator
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
role: SO:0000141
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation13/annotation13_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation13/annotation13_range/1
type: Range
persistent identity: annotation13 range
display id: annotation13_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
start: 985
end: 1061
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
role: SO:0000057
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation8/annotation8_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation8/annotation8_range/1
type: Range
persistent identity: annotation8 range
display id: annotation8_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
start: 864
end: 885
orientation: inline

lac_promoter
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation16/annotation16_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation16/annotation16_range/1
type: Range
persistent identity: annotation16 range
display id: annotation16_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
start: 820
end: 849
orientation: reverseComplement

beta-lactamase, ampicillin resistance (ampR)
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
role: SO:0000316
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation17/annotation17_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation17/annotation17_range/1
type: Range
persistent identity: annotation17 range
display id: annotation17_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
start: 2769
end: 3629
orientation: reverseComplement

AmpR promoter
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation21/annotation21_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation21/annotation21_range/1
type: Range
persistent identity: annotation21 range
display id: annotation21_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
start: 3630
end: 3734
orientation: reverseComplement

diadenosine tetraphosphatase (apaH) C-terminal fragment ( aa 198-280) AVI54590.1 from E coli MG1655
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation12/annotation12_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation12/annotation12_range/1
type: Range
persistent identity: annotation12 range
display id: annotation12_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
start: 1062
end: 1313
orientation: reverseComplement

MCS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation3/annotation3_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation3/annotation3_range/1
type: Range
persistent identity: annotation3 range
display id: annotation3_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
start: 702
end: 758
orientation: inline

lac_promoter
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation15/annotation15_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation15/annotation15_range/1
type: Range
persistent identity: annotation15 range
display id: annotation15_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
start: 1657
end: 1686
orientation: reverseComplement

E01 Translational Enhancer
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation11/annotation11_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation11/annotation11_range/1
type: Range
persistent identity: annotation11 range
display id: annotation11_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
start: 259
end: 330
orientation: inline

AmpR_promoter
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation18/annotation18_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation18/annotation18_range/1
type: Range
persistent identity: annotation18 range
display id: annotation18_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
start: 3671
end: 3699
orientation: reverseComplement

RBS
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation14/annotation14_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation14/annotation14_range/1
type: Range
persistent identity: annotation14 range
display id: annotation14_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
start: 3195
end: 3201
orientation: reverseComplement

pUC origin of replication (derivative of pBR322/pMB1/ColE1)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
role: SO:0000296
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation10/annotation10_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation10/annotation10_range/1
type: Range
persistent identity: annotation10 range
display id: annotation10_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
start: 2010
end: 2598
orientation: reverseComplement

CAP binding site
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
role: SO:0000057
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation9/annotation9_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation9/annotation9_range/1
type: Range
persistent identity: annotation9 range
display id: annotation9_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
start: 1701
end: 1722
orientation: inline

pBR322 (from pUC18)
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
role: SO:0000296
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation20/annotation20_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246/annotation20/annotation20_range/1
type: Range
persistent identity: annotation20 range
display id: annotation20_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246
start: 1995
end: 2614
orientation: reverseComplement

pEPQD0KN0244 backbone
type: ComponentDefinition
persistent identity: pEPQD0KN0244 backbone
display id: pEPQD0KN0244_backbone
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: RBS
sequence annotation: efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)
sequence annotation: NET tag (N-terminal Expression Tag), MEKKI, from chloramphenicol acetyltransferase, minimizes secondary structure with RBS and 5' UTR
sequence annotation: T7 promoter and 5' UTR from pJL1
sequence annotation: backbone from pJL1-sfGFP (Addgene #69496)
sequence annotation: T7 terminator
sequence annotation: {"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
sequence annotation: T7 promoter
sequence annotation: pBR322 (from pUC18)
sequence annotation: pUC origin of replication (derivative of pBR322/pMB1/ColE1)
sequence: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244_backbone_seq/1

RBS
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244 backbone
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244_backbone/annotation5/annotation5_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244_backbone/annotation5/annotation5_range/1
type: Range
persistent identity: annotation5 range
display id: annotation5_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244 backbone
start: 221
end: 227
orientation: inline

efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244 backbone
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244_backbone/annotation4/annotation4_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244_backbone/annotation4/annotation4_range/1
type: Range
persistent identity: annotation4 range
display id: annotation4_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244 backbone
start: 1733
end: 1755
orientation: inline

NET tag (N-terminal Expression Tag), MEKKI, from chloramphenicol acetyltransferase, minimizes secondary structure with RBS and 5' UTR
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244 backbone
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244_backbone/annotation9/annotation9_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244_backbone/annotation9/annotation9_range/1
type: Range
persistent identity: annotation9 range
display id: annotation9_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244 backbone
start: 1763
end: 1777
orientation: inline

T7 promoter and 5' UTR from pJL1
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244 backbone
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244_backbone/annotation10/annotation10_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244_backbone/annotation10/annotation10_range/1
type: Range
persistent identity: annotation10 range
display id: annotation10_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244 backbone
start: 1683
end: 1762
orientation: inline

backbone from pJL1-sfGFP (Addgene #69496)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244 backbone
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244_backbone/annotation2/annotation2_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244_backbone/annotation2/annotation2_range/1
type: Range
persistent identity: annotation2 range
display id: annotation2_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244 backbone
start: 46
end: 1679
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244 backbone
role: SO:0000141
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244_backbone/annotation3/annotation3_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244_backbone/annotation3/annotation3_range/1
type: Range
persistent identity: annotation3 range
display id: annotation3_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244 backbone
start: 65
end: 112
orientation: inline

{"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244 backbone
role: SO:0000316
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244_backbone/annotation0/annotation0_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244_backbone/annotation0/annotation0_range/1
type: Range
persistent identity: annotation0 range
display id: annotation0_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244 backbone
start: 128
end: 942
orientation: reverseComplement

T7 promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244 backbone
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244_backbone/annotation7/annotation7_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244_backbone/annotation7/annotation7_range/1
type: Range
persistent identity: annotation7 range
display id: annotation7_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244 backbone
start: 1683
end: 1701
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244 backbone
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244_backbone/annotation6/annotation6_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244_backbone/annotation6/annotation6_range/1
type: Range
persistent identity: annotation6 range
display id: annotation6_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244 backbone
start: 1748
end: 1754
orientation: inline

pBR322 (from pUC18)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244 backbone
role: SO:0000296
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244_backbone/annotation11/annotation11_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244_backbone/annotation11/annotation11_range/1
type: Range
persistent identity: annotation11 range
display id: annotation11_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244 backbone
start: 1051
end: 1670
orientation: inline

pUC origin of replication (derivative of pBR322/pMB1/ColE1)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244 backbone
role: SO:0000296
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244_backbone/annotation1/annotation1_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244_backbone/annotation1/annotation1_range/1
type: Range
persistent identity: annotation1 range
display id: annotation1_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244 backbone
start: 1067
end: 1655
orientation: inline

T7 promoter and 5' UTR from pJL1
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244 backbone
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244_backbone/annotation8/annotation8_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244_backbone/annotation8/annotation8_range/1
type: Range
persistent identity: annotation8 range
display id: annotation8_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244 backbone
start: 1683
end: 1759
orientation: inline

pEPQD0KN0244
type: ComponentDefinition
persistent identity: pEPQD0KN0244
display id: pEPQD0KN0244
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: RBS
sequence annotation: CAP binding site
sequence annotation: efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)
sequence annotation: {'lacZ-alpha', 'lacZ beta-galactosidase alpha peptide'}
sequence annotation: pUC origin of replication (derivative of pBR322/pMB1/ColE1)
sequence annotation: Plac promoter
sequence annotation: MCS
sequence annotation: {"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
sequence annotation: T7 terminator
sequence annotation: lac promoter
sequence annotation: T7 promoter and 5' UTR from pJL1
sequence annotation: T7 promoter
sequence annotation: lac operator
sequence annotation: lac_promoter
sequence annotation: NET tag (N-terminal Expression Tag), MEKKI, from chloramphenicol acetyltransferase, minimizes secondary structure with RBS and 5' UTR
sequence: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244_seq/1

RBS
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation10/annotation10_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation10/annotation10_range/1
type: Range
persistent identity: annotation10 range
display id: annotation10_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
start: 93
end: 99
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation11/annotation11_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation11/annotation11_range/1
type: Range
persistent identity: annotation11 range
display id: annotation11_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
start: 938
end: 944
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
role: SO:0000057
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation6/annotation6_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation6/annotation6_range/1
type: Range
persistent identity: annotation6 range
display id: annotation6_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
start: 636
end: 657
orientation: inline

efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation9/annotation9_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation9/annotation9_range/1
type: Range
persistent identity: annotation9 range
display id: annotation9_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
start: 78
end: 100
orientation: inline

{'lacZ-alpha', 'lacZ beta-galactosidase alpha peptide'}
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
role: SO:0000316
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation0/annotation0_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation0/annotation0_range/1
type: Range
persistent identity: annotation0 range
display id: annotation0_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
start: 224
end: 547
orientation: reverseComplement

pUC origin of replication (derivative of pBR322/pMB1/ColE1)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
role: SO:0000296
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation7/annotation7_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation7/annotation7_range/1
type: Range
persistent identity: annotation7 range
display id: annotation7_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
start: 1784
end: 2372
orientation: inline

Plac promoter
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation1/annotation1_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation1/annotation1_range/1
type: Range
persistent identity: annotation1 range
display id: annotation1_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
start: 554
end: 677
orientation: reverseComplement

MCS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation3/annotation3_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation3/annotation3_range/1
type: Range
persistent identity: annotation3 range
display id: annotation3_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
start: 474
end: 530
orientation: inline

{"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
role: SO:0000316
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation2/annotation2_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation2/annotation2_range/1
type: Range
persistent identity: annotation2 range
display id: annotation2_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
start: 847
end: 1662
orientation: reverseComplement

T7 terminator
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
role: SO:0000141
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation8/annotation8_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation8/annotation8_range/1
type: Range
persistent identity: annotation8 range
display id: annotation8_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
start: 782
end: 829
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation5/annotation5_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation5/annotation5_range/1
type: Range
persistent identity: annotation5 range
display id: annotation5_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
start: 591
end: 621
orientation: reverseComplement

T7 promoter and 5' UTR from pJL1
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation16/annotation16_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation16/annotation16_range/1
type: Range
persistent identity: annotation16 range
display id: annotation16_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
start: 28
end: 107
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation12/annotation12_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation12/annotation12_range/1
type: Range
persistent identity: annotation12 range
display id: annotation12_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
start: 28
end: 46
orientation: inline

T7 promoter and 5' UTR from pJL1
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation13/annotation13_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation13/annotation13_range/1
type: Range
persistent identity: annotation13 range
display id: annotation13_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
start: 28
end: 104
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
role: SO:0000057
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation4/annotation4_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation4/annotation4_range/1
type: Range
persistent identity: annotation4 range
display id: annotation4_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
start: 567
end: 583
orientation: inline

lac_promoter
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation15/annotation15_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation15/annotation15_range/1
type: Range
persistent identity: annotation15 range
display id: annotation15_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
start: 592
end: 621
orientation: reverseComplement

NET tag (N-terminal Expression Tag), MEKKI, from chloramphenicol acetyltransferase, minimizes secondary structure with RBS and 5' UTR
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation14/annotation14_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244/annotation14/annotation14_range/1
type: Range
persistent identity: annotation14 range
display id: annotation14_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0244
start: 108
end: 122
orientation: inline

pEPQD0KN0024 backbone
type: ComponentDefinition
persistent identity: pEPQD0KN0024 backbone
display id: pEPQD0KN0024_backbone
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)
sequence annotation: RBS
sequence annotation: backbone from pJL1-sfGFP (Addgene #69496)
sequence annotation: NET tag (N-terminal Expression Tag), MEKKI, from chloramphenicol acetyltransferase, minimizes secondary structure with RBS and 5' UTR
sequence annotation: T7 terminator
sequence annotation: T7 promoter
sequence annotation: pBR322 (from pUC18)
sequence annotation: {"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
sequence annotation: T7 promoter and 5' UTR from pJL1
sequence annotation: pUC origin of replication (derivative of pBR322/pMB1/ColE1)
sequence: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024_backbone_seq/1

efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024 backbone
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024_backbone/annotation4/annotation4_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024_backbone/annotation4/annotation4_range/1
type: Range
persistent identity: annotation4 range
display id: annotation4_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024 backbone
start: 1733
end: 1755
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024 backbone
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024_backbone/annotation6/annotation6_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024_backbone/annotation6/annotation6_range/1
type: Range
persistent identity: annotation6 range
display id: annotation6_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024 backbone
start: 1748
end: 1754
orientation: inline

backbone from pJL1-sfGFP (Addgene #69496)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024 backbone
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024_backbone/annotation2/annotation2_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024_backbone/annotation2/annotation2_range/1
type: Range
persistent identity: annotation2 range
display id: annotation2_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024 backbone
start: 46
end: 1679
orientation: inline

NET tag (N-terminal Expression Tag), MEKKI, from chloramphenicol acetyltransferase, minimizes secondary structure with RBS and 5' UTR
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024 backbone
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024_backbone/annotation9/annotation9_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024_backbone/annotation9/annotation9_range/1
type: Range
persistent identity: annotation9 range
display id: annotation9_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024 backbone
start: 1763
end: 1777
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024 backbone
role: SO:0000141
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024_backbone/annotation3/annotation3_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024_backbone/annotation3/annotation3_range/1
type: Range
persistent identity: annotation3 range
display id: annotation3_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024 backbone
start: 65
end: 112
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024 backbone
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024_backbone/annotation7/annotation7_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024_backbone/annotation7/annotation7_range/1
type: Range
persistent identity: annotation7 range
display id: annotation7_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
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top level: pEPQD0KN0024 backbone
start: 1683
end: 1701
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024 backbone
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024_backbone/annotation5/annotation5_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024_backbone/annotation5/annotation5_range/1
type: Range
persistent identity: annotation5 range
display id: annotation5_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
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top level: pEPQD0KN0024 backbone
start: 221
end: 227
orientation: inline

pBR322 (from pUC18)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024 backbone
role: SO:0000296
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024_backbone/annotation11/annotation11_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024_backbone/annotation11/annotation11_range/1
type: Range
persistent identity: annotation11 range
display id: annotation11_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
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top level: pEPQD0KN0024 backbone
start: 1051
end: 1670
orientation: inline

{"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024 backbone
role: SO:0000316
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024_backbone/annotation0/annotation0_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024_backbone/annotation0/annotation0_range/1
type: Range
persistent identity: annotation0 range
display id: annotation0_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024 backbone
start: 128
end: 942
orientation: reverseComplement

T7 promoter and 5' UTR from pJL1
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024 backbone
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024_backbone/annotation10/annotation10_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024_backbone/annotation10/annotation10_range/1
type: Range
persistent identity: annotation10 range
display id: annotation10_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
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top level: pEPQD0KN0024 backbone
start: 1683
end: 1762
orientation: inline

T7 promoter and 5' UTR from pJL1
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024 backbone
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024_backbone/annotation8/annotation8_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024_backbone/annotation8/annotation8_range/1
type: Range
persistent identity: annotation8 range
display id: annotation8_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024 backbone
start: 1683
end: 1759
orientation: inline

pUC origin of replication (derivative of pBR322/pMB1/ColE1)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024 backbone
role: SO:0000296
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024_backbone/annotation1/annotation1_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024_backbone/annotation1/annotation1_range/1
type: Range
persistent identity: annotation1 range
display id: annotation1_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024 backbone
start: 1067
end: 1655
orientation: inline

pEPQD0CB0246 backbone
type: ComponentDefinition
persistent identity: pEPQD0CB0246 backbone
display id: pEPQD0CB0246_backbone
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: lac operator
sequence annotation: Plac promoter
sequence annotation: AmpR_promoter
sequence annotation: pBR322 (from pUC18)
sequence annotation: beta-lactamase, ampicillin resistance (ampR)
sequence annotation: folA/apaH terminator
sequence annotation: CAP binding site
sequence annotation: E01 Translational Enhancer
sequence annotation: RBS
sequence annotation: AmpR promoter
sequence annotation: lac_promoter
sequence annotation: lac promoter
sequence annotation: pUC origin of replication (derivative of pBR322/pMB1/ColE1)
sequence annotation: diadenosine tetraphosphatase (apaH) C-terminal fragment ( aa 198-280) AVI54590.1 from E coli MG1655
sequence annotation: {'tet (887 - 636)', 'tet'}
sequence: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone_seq/1

lac operator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
role: SO:0000057
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation1/annotation1_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation1/annotation1_range/1
type: Range
persistent identity: annotation1 range
display id: annotation1_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
start: 687
end: 703
orientation: inline

Plac promoter
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation0/annotation0_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation0/annotation0_range/1
type: Range
persistent identity: annotation0 range
display id: annotation0_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
start: 672
end: 794
orientation: reverseComplement

AmpR_promoter
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation11/annotation11_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation11/annotation11_range/1
type: Range
persistent identity: annotation11 range
display id: annotation11_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
start: 2724
end: 2751
orientation: reverseComplement

pBR322 (from pUC18)
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
role: SO:0000296
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation13/annotation13_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation13/annotation13_range/1
type: Range
persistent identity: annotation13 range
display id: annotation13_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
start: 1048
end: 1666
orientation: reverseComplement

beta-lactamase, ampicillin resistance (ampR)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
role: SO:0000316
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation10/annotation10_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation10/annotation10_range/1
type: Range
persistent identity: annotation10 range
display id: annotation10_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
start: 1822
end: 2681
orientation: reverseComplement

folA/apaH terminator
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
role: SO:0000141
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation7/annotation7_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation7/annotation7_range/1
type: Range
persistent identity: annotation7 range
display id: annotation7_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
start: 40
end: 116
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
role: SO:0000057
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation3/annotation3_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation3/annotation3_range/1
type: Range
persistent identity: annotation3 range
display id: annotation3_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
start: 756
end: 777
orientation: inline

E01 Translational Enhancer
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation5/annotation5_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation5/annotation5_range/1
type: Range
persistent identity: annotation5 range
display id: annotation5_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
start: 3595
end: 3666
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation8/annotation8_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation8/annotation8_range/1
type: Range
persistent identity: annotation8 range
display id: annotation8_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
start: 2248
end: 2253
orientation: reverseComplement

AmpR promoter
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation14/annotation14_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation14/annotation14_range/1
type: Range
persistent identity: annotation14 range
display id: annotation14_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
start: 2683
end: 2786
orientation: reverseComplement

lac_promoter
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation9/annotation9_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation9/annotation9_range/1
type: Range
persistent identity: annotation9 range
display id: annotation9_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
start: 710
end: 738
orientation: reverseComplement

lac promoter
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation2/annotation2_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation2/annotation2_range/1
type: Range
persistent identity: annotation2 range
display id: annotation2_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
start: 709
end: 738
orientation: reverseComplement

pUC origin of replication (derivative of pBR322/pMB1/ColE1)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
role: SO:0000296
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation4/annotation4_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation4/annotation4_range/1
type: Range
persistent identity: annotation4 range
display id: annotation4_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
start: 1063
end: 1650
orientation: reverseComplement

diadenosine tetraphosphatase (apaH) C-terminal fragment ( aa 198-280) AVI54590.1 from E coli MG1655
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation6/annotation6_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation6/annotation6_range/1
type: Range
persistent identity: annotation6 range
display id: annotation6_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
start: 115
end: 365
orientation: reverseComplement

{'tet (887 - 636)', 'tet'}
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
role: SO:0000110
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation12/annotation12_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone/annotation12/annotation12_range/1
type: Range
persistent identity: annotation12 range
display id: annotation12_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0246 backbone
start: 3083
end: 3333
orientation: reverseComplement

pEPQD0KN0024
type: ComponentDefinition
persistent identity: pEPQD0KN0024
display id: pEPQD0KN0024
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: NET tag (N-terminal Expression Tag), MEKKI, from chloramphenicol acetyltransferase, minimizes secondary structure with RBS and 5' UTR
sequence annotation: lac promoter
sequence annotation: CAP binding site
sequence annotation: {"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
sequence annotation: pUC origin of replication (derivative of pBR322/pMB1/ColE1)
sequence annotation: lac operator
sequence annotation: T7 promoter
sequence annotation: T7 promoter and 5' UTR from pJL1
sequence annotation: MCS
sequence annotation: RBS
sequence annotation: efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)
sequence annotation: lac_promoter
sequence annotation: {'lacZ-alpha', 'lacZ beta-galactosidase alpha peptide'}
sequence annotation: T7 terminator
sequence annotation: Plac promoter
sequence: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024_seq/1

NET tag (N-terminal Expression Tag), MEKKI, from chloramphenicol acetyltransferase, minimizes secondary structure with RBS and 5' UTR
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation14/annotation14_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation14/annotation14_range/1
type: Range
persistent identity: annotation14 range
display id: annotation14_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
start: 108
end: 122
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation5/annotation5_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation5/annotation5_range/1
type: Range
persistent identity: annotation5 range
display id: annotation5_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
start: 589
end: 619
orientation: reverseComplement

CAP binding site
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
role: SO:0000057
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation6/annotation6_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation6/annotation6_range/1
type: Range
persistent identity: annotation6 range
display id: annotation6_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
start: 634
end: 655
orientation: inline

{"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
role: SO:0000316
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation2/annotation2_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation2/annotation2_range/1
type: Range
persistent identity: annotation2 range
display id: annotation2_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
start: 845
end: 1660
orientation: reverseComplement

pUC origin of replication (derivative of pBR322/pMB1/ColE1)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
role: SO:0000296
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation7/annotation7_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation7/annotation7_range/1
type: Range
persistent identity: annotation7 range
display id: annotation7_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
start: 1782
end: 2370
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
role: SO:0000057
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation4/annotation4_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation4/annotation4_range/1
type: Range
persistent identity: annotation4 range
display id: annotation4_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
start: 565
end: 581
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation12/annotation12_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation12/annotation12_range/1
type: Range
persistent identity: annotation12 range
display id: annotation12_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
start: 28
end: 46
orientation: inline

T7 promoter and 5' UTR from pJL1
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation13/annotation13_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation13/annotation13_range/1
type: Range
persistent identity: annotation13 range
display id: annotation13_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
start: 28
end: 104
orientation: inline

MCS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation3/annotation3_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation3/annotation3_range/1
type: Range
persistent identity: annotation3 range
display id: annotation3_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
start: 472
end: 528
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation11/annotation11_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation11/annotation11_range/1
type: Range
persistent identity: annotation11 range
display id: annotation11_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
start: 936
end: 942
orientation: inline

efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation9/annotation9_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation9/annotation9_range/1
type: Range
persistent identity: annotation9 range
display id: annotation9_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
start: 78
end: 100
orientation: inline

lac_promoter
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation15/annotation15_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation15/annotation15_range/1
type: Range
persistent identity: annotation15 range
display id: annotation15_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
start: 590
end: 619
orientation: reverseComplement

RBS
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation10/annotation10_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation10/annotation10_range/1
type: Range
persistent identity: annotation10 range
display id: annotation10_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
start: 93
end: 99
orientation: inline

{'lacZ-alpha', 'lacZ beta-galactosidase alpha peptide'}
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
role: SO:0000316
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation0/annotation0_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation0/annotation0_range/1
type: Range
persistent identity: annotation0 range
display id: annotation0_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
start: 222
end: 545
orientation: reverseComplement

T7 promoter and 5' UTR from pJL1
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation16/annotation16_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation16/annotation16_range/1
type: Range
persistent identity: annotation16 range
display id: annotation16_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
start: 28
end: 107
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
role: SO:0000141
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation8/annotation8_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation8/annotation8_range/1
type: Range
persistent identity: annotation8 range
display id: annotation8_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
start: 780
end: 827
orientation: inline

Plac promoter
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation1/annotation1_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024/annotation1/annotation1_range/1
type: Range
persistent identity: annotation1 range
display id: annotation1_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0024
start: 552
end: 675
orientation: reverseComplement

pEPQD0KN0245 backbone
type: ComponentDefinition
persistent identity: pEPQD0KN0245 backbone
display id: pEPQD0KN0245_backbone
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: T7 promoter and 5' UTR from pJL1
sequence annotation: {"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
sequence annotation: T7 terminator
sequence annotation: efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)
sequence annotation: RBS
sequence annotation: pBR322 (from pUC18)
sequence annotation: backbone from pJL1-sfGFP (Addgene #69496)
sequence annotation: T7 promoter
sequence annotation: pUC origin of replication (derivative of pBR322/pMB1/ColE1)
sequence: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245_backbone_seq/1

T7 promoter and 5' UTR from pJL1
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245 backbone
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245_backbone/annotation8/annotation8_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245_backbone/annotation8/annotation8_range/1
type: Range
persistent identity: annotation8 range
display id: annotation8_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245 backbone
start: 1683
end: 1759
orientation: inline

{"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245 backbone
role: SO:0000316
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245_backbone/annotation0/annotation0_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245_backbone/annotation0/annotation0_range/1
type: Range
persistent identity: annotation0 range
display id: annotation0_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245 backbone
start: 128
end: 942
orientation: reverseComplement

T7 terminator
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245 backbone
role: SO:0000141
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245_backbone/annotation3/annotation3_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245_backbone/annotation3/annotation3_range/1
type: Range
persistent identity: annotation3 range
display id: annotation3_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245 backbone
start: 65
end: 112
orientation: inline

efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245 backbone
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245_backbone/annotation4/annotation4_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245_backbone/annotation4/annotation4_range/1
type: Range
persistent identity: annotation4 range
display id: annotation4_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245 backbone
start: 1733
end: 1755
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245 backbone
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245_backbone/annotation5/annotation5_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245_backbone/annotation5/annotation5_range/1
type: Range
persistent identity: annotation5 range
display id: annotation5_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245 backbone
start: 221
end: 227
orientation: inline

pBR322 (from pUC18)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245 backbone
role: SO:0000296
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245_backbone/annotation9/annotation9_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245_backbone/annotation9/annotation9_range/1
type: Range
persistent identity: annotation9 range
display id: annotation9_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245 backbone
start: 1051
end: 1670
orientation: inline

backbone from pJL1-sfGFP (Addgene #69496)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245 backbone
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245_backbone/annotation2/annotation2_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245_backbone/annotation2/annotation2_range/1
type: Range
persistent identity: annotation2 range
display id: annotation2_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245 backbone
start: 46
end: 1679
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245 backbone
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245_backbone/annotation7/annotation7_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245_backbone/annotation7/annotation7_range/1
type: Range
persistent identity: annotation7 range
display id: annotation7_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245 backbone
start: 1683
end: 1701
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245 backbone
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245_backbone/annotation6/annotation6_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245_backbone/annotation6/annotation6_range/1
type: Range
persistent identity: annotation6 range
display id: annotation6_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245 backbone
start: 1748
end: 1754
orientation: inline

pUC origin of replication (derivative of pBR322/pMB1/ColE1)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245 backbone
role: SO:0000296
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245_backbone/annotation1/annotation1_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245_backbone/annotation1/annotation1_range/1
type: Range
persistent identity: annotation1 range
display id: annotation1_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0245 backbone
start: 1067
end: 1655
orientation: inline

pEPQD0KN0284 backbone
type: ComponentDefinition
persistent identity: pEPQD0KN0284 backbone
display id: pEPQD0KN0284_backbone
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: folA/apaH terminator
sequence annotation: E01 Translational Enhancer
sequence annotation: backbone from pJL1-sfGFP (Addgene #69496)
sequence annotation: diadenosine tetraphosphatase (apaH) C-terminal fragment ( aa 198-280) AVI54590.1 from E coli MG1655
sequence annotation: pUC origin of replication (derivative of pBR322/pMB1/ColE1)
sequence annotation: {"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
sequence annotation: T7 terminator
sequence annotation: RBS
sequence annotation: pBR322 (from pUC18)
sequence: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284_backbone_seq/1

folA/apaH terminator
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284 backbone
role: SO:0000141
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284_backbone/annotation6/annotation6_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284_backbone/annotation6/annotation6_range/1
type: Range
persistent identity: annotation6 range
display id: annotation6_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284 backbone
start: 40
end: 116
orientation: inline

E01 Translational Enhancer
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284 backbone
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284_backbone/annotation4/annotation4_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284_backbone/annotation4/annotation4_range/1
type: Range
persistent identity: annotation4 range
display id: annotation4_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284 backbone
start: 2440
end: 2511
orientation: inline

backbone from pJL1-sfGFP (Addgene #69496)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284 backbone
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284_backbone/annotation2/annotation2_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284_backbone/annotation2/annotation2_range/1
type: Range
persistent identity: annotation2 range
display id: annotation2_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284 backbone
start: 694
end: 2327
orientation: inline

diadenosine tetraphosphatase (apaH) C-terminal fragment ( aa 198-280) AVI54590.1 from E coli MG1655
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284 backbone
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284_backbone/annotation5/annotation5_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284_backbone/annotation5/annotation5_range/1
type: Range
persistent identity: annotation5 range
display id: annotation5_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284 backbone
start: 115
end: 365
orientation: reverseComplement

pUC origin of replication (derivative of pBR322/pMB1/ColE1)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284 backbone
role: SO:0000296
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284_backbone/annotation1/annotation1_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284_backbone/annotation1/annotation1_range/1
type: Range
persistent identity: annotation1 range
display id: annotation1_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284 backbone
start: 1715
end: 2303
orientation: inline

{"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284 backbone
role: SO:0000316
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284_backbone/annotation0/annotation0_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284_backbone/annotation0/annotation0_range/1
type: Range
persistent identity: annotation0 range
display id: annotation0_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284 backbone
start: 776
end: 1590
orientation: reverseComplement

T7 terminator
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284 backbone
role: SO:0000141
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284_backbone/annotation3/annotation3_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284_backbone/annotation3/annotation3_range/1
type: Range
persistent identity: annotation3 range
display id: annotation3_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284 backbone
start: 713
end: 760
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284 backbone
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284_backbone/annotation7/annotation7_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284_backbone/annotation7/annotation7_range/1
type: Range
persistent identity: annotation7 range
display id: annotation7_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284 backbone
start: 869
end: 875
orientation: inline

pBR322 (from pUC18)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284 backbone
role: SO:0000296
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284_backbone/annotation8/annotation8_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284_backbone/annotation8/annotation8_range/1
type: Range
persistent identity: annotation8 range
display id: annotation8_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0284 backbone
start: 1699
end: 2318
orientation: inline

pEPQD0KN0282
type: ComponentDefinition
persistent identity: pEPQD0KN0282
display id: pEPQD0KN0282
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: {'lacZ-alpha', 'lacZ beta-galactosidase alpha peptide'}
sequence annotation: lac_promoter
sequence annotation: lac operator
sequence annotation: E01 Translational Enhancer
sequence annotation: RBS
sequence annotation: pBR322 (from pUC18)
sequence annotation: lac promoter
sequence annotation: {"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
sequence annotation: MCS
sequence annotation: T7 terminator
sequence annotation: pUC origin of replication (derivative of pBR322/pMB1/ColE1)
sequence annotation: CAP binding site
sequence annotation: Plac promoter
sequence: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282_seq/1

{'lacZ-alpha', 'lacZ beta-galactosidase alpha peptide'}
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0282
role: SO:0000316
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282/annotation0/annotation0_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282/annotation0/annotation0_range/1
type: Range
persistent identity: annotation0 range
display id: annotation0_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0282
start: 315
end: 638
orientation: reverseComplement

lac_promoter
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0282
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282/annotation11/annotation11_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282/annotation11/annotation11_range/1
type: Range
persistent identity: annotation11 range
display id: annotation11_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0282
start: 683
end: 712
orientation: reverseComplement

lac operator
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0282
role: SO:0000057
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282/annotation4/annotation4_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282/annotation4/annotation4_range/1
type: Range
persistent identity: annotation4 range
display id: annotation4_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0282
start: 658
end: 674
orientation: inline

E01 Translational Enhancer
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0282
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282/annotation9/annotation9_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282/annotation9/annotation9_range/1
type: Range
persistent identity: annotation9 range
display id: annotation9_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0282
start: 122
end: 193
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0282
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282/annotation10/annotation10_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282/annotation10/annotation10_range/1
type: Range
persistent identity: annotation10 range
display id: annotation10_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0282
start: 1029
end: 1035
orientation: inline

pBR322 (from pUC18)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0282
role: SO:0000296
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282/annotation12/annotation12_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282/annotation12/annotation12_range/1
type: Range
persistent identity: annotation12 range
display id: annotation12_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0282
start: 1859
end: 2478
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0282
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282/annotation5/annotation5_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282/annotation5/annotation5_range/1
type: Range
persistent identity: annotation5 range
display id: annotation5_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0282
start: 682
end: 712
orientation: reverseComplement

{"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0282
role: SO:0000316
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282/annotation2/annotation2_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282/annotation2/annotation2_range/1
type: Range
persistent identity: annotation2 range
display id: annotation2_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0282
start: 938
end: 1753
orientation: reverseComplement

MCS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0282
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282/annotation3/annotation3_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282/annotation3/annotation3_range/1
type: Range
persistent identity: annotation3 range
display id: annotation3_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0282
start: 565
end: 621
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0282
role: SO:0000141
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282/annotation8/annotation8_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282/annotation8/annotation8_range/1
type: Range
persistent identity: annotation8 range
display id: annotation8_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0282
start: 873
end: 920
orientation: inline

pUC origin of replication (derivative of pBR322/pMB1/ColE1)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0282
role: SO:0000296
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282/annotation7/annotation7_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282/annotation7/annotation7_range/1
type: Range
persistent identity: annotation7 range
display id: annotation7_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0282
start: 1875
end: 2463
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0282
role: SO:0000057
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282/annotation6/annotation6_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282/annotation6/annotation6_range/1
type: Range
persistent identity: annotation6 range
display id: annotation6_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0282
start: 727
end: 748
orientation: inline

Plac promoter
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0282
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282/annotation1/annotation1_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282/annotation1/annotation1_range/1
type: Range
persistent identity: annotation1 range
display id: annotation1_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0282
start: 645
end: 768
orientation: reverseComplement

pEPQD0CB0026
type: ComponentDefinition
persistent identity: pEPQD0CB0026
display id: pEPQD0CB0026
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: lac operator
sequence annotation: lac_promoter
sequence annotation: RBS
sequence annotation: CAP binding site
sequence annotation: pUC origin of replication (derivative of pBR322/pMB1/ColE1)
sequence annotation: lac promoter
sequence annotation: Plac promoter
sequence annotation: pBR322 (from pUC18)
sequence annotation: AmpR promoter
sequence annotation: AmpR_promoter
sequence annotation: MCS
sequence annotation: beta-lactamase, ampicillin resistance (ampR)
sequence annotation: diadenosine tetraphosphatase (apaH) C-terminal fragment ( aa 198-280) AVI54590.1 from E coli MG1655
sequence annotation: {'tet (887 - 636)', 'tet'}
sequence annotation: folA/apaH terminator
sequence annotation: E01 Translational Enhancer
sequence annotation: {'lacZ-alpha', 'lacZ beta-galactosidase alpha peptide'}
sequence: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_seq/1

lac operator
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
role: SO:0000057
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation5/annotation5_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation5/annotation5_range/1
type: Range
persistent identity: annotation5 range
display id: annotation5_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
start: 1632
end: 1648
orientation: inline

lac_promoter
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation15/annotation15_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation15/annotation15_range/1
type: Range
persistent identity: annotation15 range
display id: annotation15_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
start: 1657
end: 1686
orientation: reverseComplement

RBS
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation14/annotation14_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation14/annotation14_range/1
type: Range
persistent identity: annotation14 range
display id: annotation14_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
start: 3195
end: 3201
orientation: reverseComplement

CAP binding site
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
role: SO:0000057
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation8/annotation8_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation8/annotation8_range/1
type: Range
persistent identity: annotation8 range
display id: annotation8_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
start: 864
end: 885
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
role: SO:0000057
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation9/annotation9_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation9/annotation9_range/1
type: Range
persistent identity: annotation9 range
display id: annotation9_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
start: 1701
end: 1722
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
role: SO:0000057
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation4/annotation4_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation4/annotation4_range/1
type: Range
persistent identity: annotation4 range
display id: annotation4_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
start: 795
end: 811
orientation: inline

pUC origin of replication (derivative of pBR322/pMB1/ColE1)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
role: SO:0000296
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation10/annotation10_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation10/annotation10_range/1
type: Range
persistent identity: annotation10 range
display id: annotation10_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
start: 2010
end: 2598
orientation: reverseComplement

lac promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation7/annotation7_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation7/annotation7_range/1
type: Range
persistent identity: annotation7 range
display id: annotation7_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
start: 819
end: 849
orientation: reverseComplement

Plac promoter
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation1/annotation1_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation1/annotation1_range/1
type: Range
persistent identity: annotation1 range
display id: annotation1_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
start: 1619
end: 1742
orientation: reverseComplement

pBR322 (from pUC18)
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
role: SO:0000296
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation20/annotation20_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation20/annotation20_range/1
type: Range
persistent identity: annotation20 range
display id: annotation20_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
start: 1995
end: 2614
orientation: reverseComplement

AmpR promoter
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation21/annotation21_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation21/annotation21_range/1
type: Range
persistent identity: annotation21 range
display id: annotation21_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
start: 3630
end: 3734
orientation: reverseComplement

AmpR_promoter
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
role: SO:0000167
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http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation18/annotation18_range/1
type: Range
persistent identity: annotation18 range
display id: annotation18_range
version: 1
OBI_0001617: 34693026
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owned by: fxbuson
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top level: pEPQD0CB0026
start: 3671
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orientation: reverseComplement

Plac promoter
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
OBI_0001617: 34693026
owned by: ct677
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top level: pEPQD0CB0026
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http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation2/annotation2_range/1
type: Range
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display id: annotation2_range
version: 1
OBI_0001617: 34693026
owned by: ct677
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top level: pEPQD0CB0026
start: 782
end: 905
orientation: reverseComplement

lac_promoter
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
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top level: pEPQD0CB0026
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location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation16/annotation16_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation16/annotation16_range/1
type: Range
persistent identity: annotation16 range
display id: annotation16_range
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OBI_0001617: 34693026
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top level: pEPQD0CB0026
start: 820
end: 849
orientation: reverseComplement

MCS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
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location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation3/annotation3_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation3/annotation3_range/1
type: Range
persistent identity: annotation3 range
display id: annotation3_range
version: 1
OBI_0001617: 34693026
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top level: pEPQD0CB0026
start: 702
end: 758
orientation: inline

beta-lactamase, ampicillin resistance (ampR)
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
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top level: pEPQD0CB0026
role: SO:0000316
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation17/annotation17_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation17/annotation17_range/1
type: Range
persistent identity: annotation17 range
display id: annotation17_range
version: 1
OBI_0001617: 34693026
owned by: ct677
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top level: pEPQD0CB0026
start: 2769
end: 3629
orientation: reverseComplement

diadenosine tetraphosphatase (apaH) C-terminal fragment ( aa 198-280) AVI54590.1 from E coli MG1655
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
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top level: pEPQD0CB0026
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation12/annotation12_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation12/annotation12_range/1
type: Range
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display id: annotation12_range
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OBI_0001617: 34693026
owned by: ct677
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top level: pEPQD0CB0026
start: 1062
end: 1313
orientation: reverseComplement

{'tet (887 - 636)', 'tet'}
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
role: SO:0000110
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation19/annotation19_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation19/annotation19_range/1
type: Range
persistent identity: annotation19 range
display id: annotation19_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
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top level: pEPQD0CB0026
start: 4030
end: 4281
orientation: reverseComplement

folA/apaH terminator
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
role: SO:0000141
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation13/annotation13_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation13/annotation13_range/1
type: Range
persistent identity: annotation13 range
display id: annotation13_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
start: 985
end: 1061
orientation: inline

E01 Translational Enhancer
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation11/annotation11_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation11/annotation11_range/1
type: Range
persistent identity: annotation11 range
display id: annotation11_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
start: 259
end: 330
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation6/annotation6_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation6/annotation6_range/1
type: Range
persistent identity: annotation6 range
display id: annotation6_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
start: 1656
end: 1686
orientation: reverseComplement

{'lacZ-alpha', 'lacZ beta-galactosidase alpha peptide'}
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
role: SO:0000316
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation0/annotation0_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026/annotation0/annotation0_range/1
type: Range
persistent identity: annotation0 range
display id: annotation0_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0CB0026
start: 452
end: 775
orientation: reverseComplement

pEPQD0KN0025
type: ComponentDefinition
persistent identity: pEPQD0KN0025
display id: pEPQD0KN0025
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: Plac promoter
sequence annotation: {'lacZ-alpha', 'lacZ beta-galactosidase alpha peptide'}
sequence annotation: T7 promoter and 5' UTR from pJL1
sequence annotation: lac promoter
sequence annotation: RBS
sequence annotation: pUC origin of replication (derivative of pBR322/pMB1/ColE1)
sequence annotation: lac_promoter
sequence annotation: MCS
sequence annotation: lac operator
sequence annotation: CAP binding site
sequence annotation: T7 terminator
sequence annotation: efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)
sequence annotation: T7 promoter
sequence annotation: {"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
sequence: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025_seq/1

Plac promoter
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation1/annotation1_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation1/annotation1_range/1
type: Range
persistent identity: annotation1 range
display id: annotation1_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
start: 537
end: 660
orientation: reverseComplement

{'lacZ-alpha', 'lacZ beta-galactosidase alpha peptide'}
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
role: SO:0000316
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation0/annotation0_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation0/annotation0_range/1
type: Range
persistent identity: annotation0 range
display id: annotation0_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
start: 207
end: 530
orientation: reverseComplement

T7 promoter and 5' UTR from pJL1
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation13/annotation13_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation13/annotation13_range/1
type: Range
persistent identity: annotation13 range
display id: annotation13_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
start: 28
end: 104
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation5/annotation5_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation5/annotation5_range/1
type: Range
persistent identity: annotation5 range
display id: annotation5_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
start: 574
end: 604
orientation: reverseComplement

RBS
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation10/annotation10_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation10/annotation10_range/1
type: Range
persistent identity: annotation10 range
display id: annotation10_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
start: 93
end: 99
orientation: inline

pUC origin of replication (derivative of pBR322/pMB1/ColE1)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
role: SO:0000296
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation7/annotation7_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation7/annotation7_range/1
type: Range
persistent identity: annotation7 range
display id: annotation7_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
start: 1767
end: 2355
orientation: inline

lac_promoter
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation14/annotation14_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation14/annotation14_range/1
type: Range
persistent identity: annotation14 range
display id: annotation14_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
start: 575
end: 604
orientation: reverseComplement

MCS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
role: SO:0000804
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation3/annotation3_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation3/annotation3_range/1
type: Range
persistent identity: annotation3 range
display id: annotation3_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
start: 457
end: 513
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation11/annotation11_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation11/annotation11_range/1
type: Range
persistent identity: annotation11 range
display id: annotation11_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
start: 921
end: 927
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
role: SO:0000057
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation4/annotation4_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation4/annotation4_range/1
type: Range
persistent identity: annotation4 range
display id: annotation4_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
start: 550
end: 566
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
role: SO:0000057
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation6/annotation6_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation6/annotation6_range/1
type: Range
persistent identity: annotation6 range
display id: annotation6_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
start: 619
end: 640
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
role: SO:0000141
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation8/annotation8_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation8/annotation8_range/1
type: Range
persistent identity: annotation8 range
display id: annotation8_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
start: 765
end: 812
orientation: inline

efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
role: SO:0000139
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation9/annotation9_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation9/annotation9_range/1
type: Range
persistent identity: annotation9 range
display id: annotation9_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
start: 78
end: 100
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
role: SO:0000167
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation12/annotation12_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation12/annotation12_range/1
type: Range
persistent identity: annotation12 range
display id: annotation12_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
start: 28
end: 46
orientation: inline

{"aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)", 'KanR'}
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
role: SO:0000316
location: http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation2/annotation2_range/1

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025/annotation2/annotation2_range/1
type: Range
persistent identity: annotation2 range
display id: annotation2_range
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
top level: pEPQD0KN0025
start: 830
end: 1645
orientation: reverseComplement

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_backbone_seq/1
type: Sequence
persistent identity: pEPQD0CB0246 backbone seq
display id: pEPQD0CB0246_backbone_seq
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
elements: GCTTCGGGTTATAATTACCTCAGGTCGACGTCCCATGGTTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCTATATCAGGCGGTGTTTAAGACGCCGCCGCTTCGCCCAAATCCTTATGCCGGTTCGACGACTGGACAAAATACTGTTTATCTTCCCAGCGCAGGCAGGTTAATGTACCACCCCAGCAGCAGCCGGTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTCAGTGGGGCAGGGGCCTCTTCCGGCGATTCTTACTAGCTAGTATGCATAGGTGCTGAAATATAAAGTTTGTGTTTCTAAAACACACGTGGTACGTACGATAACGTACAGTGTTTTTCCCTCCACTTAAATCGAAGGGTAGTGTCTTGGAGCGCGCGGAGTAAACATATATGGTTCATATATGTCCGTAGGCACGTAAAAAAAGCGAGGGATTCGAATTCCCCCGGAACCCCCGGTTGGGGCCCACGCCTCGATCGAGCAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAGCTTTCCCGCGGCCAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCACTCACTGACTCGCTGCGCTCGGTCGCTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGATCCACGCTCACCGGCTCCAGATTTATCAGCAATA…
encoding: naseq.html

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025_backbone_seq/1
type: Sequence
persistent identity: pEPQD0KN0025 backbone seq
display id: pEPQD0KN0025_backbone_seq
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
elements: GCTTCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGCCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCTTCGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGATCCCGCGAAATTAATACGACTCACTATAGGGACACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACAAATG
encoding: naseq.html

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0025_seq/1
type: Sequence
persistent identity: pEPQD0KN0025 seq
display id: pEPQD0KN0025_seq
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
elements: AACGCCAGCAACGCGATCCCGCGAAATTAATACGACTCACTATAGGGACACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACAAATGTGAGACCGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTAGGTCTCAGCTTCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGCCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCTTCGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGT…
encoding: naseq.html

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024_backbone_seq/1
type: Sequence
persistent identity: pEPQD0KN0024 backbone seq
display id: pEPQD0KN0024_backbone_seq
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
elements: GCTTCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGCCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCTTCGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGATCCCGCGAAATTAATACGACTCACTATAGGGACACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGAGAAAAAAATAATG
encoding: naseq.html

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282_backbone_seq/1
type: Sequence
persistent identity: pEPQD0KN0282 backbone seq
display id: pEPQD0KN0282_backbone_seq
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
elements: GCTTCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGCCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCTTCGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGATCCCGCGACGAATAAGCCAGATGCTACACAATTAGGCTTGTACATACTGTCGTTAGAACGCGGCTACAATTAATACATAACCTTATGTATCATACACATACGATTTAGGTGACACTATAGAACTCACCTATCTCCCCAACACCTAATAACATTCAATCACTCTTTCCACTAACCACCTATCTACATCACCAAGATATCACTAGTTCTCGAGCTAATG
encoding: naseq.html

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0282_seq/1
type: Sequence
persistent identity: pEPQD0KN0282 seq
display id: pEPQD0KN0282_seq
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
elements: ATCCCGCGACGAATAAGCCAGATGCTACACAATTAGGCTTGTACATACTGTCGTTAGAACGCGGCTACAATTAATACATAACCTTATGTATCATACACATACGATTTAGGTGACACTATAGAACTCACCTATCTCCCCAACACCTAATAACATTCAATCACTCTTTCCACTAACCACCTATCTACATCACCAAGATATCACTAGTTCTCGAGCTAATGAGAGACCGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTAGGTCTCAGCTTCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGCCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCTTCGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAG…
encoding: naseq.html

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_backbone_seq/1
type: Sequence
persistent identity: pEPQD0CB0026 backbone seq
display id: pEPQD0CB0026_backbone_seq
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
elements: GCTTCGGGTTATAATTACCTCAGGTCGACGTCCCATGGTTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCTATATCAGGCGGTGTTTAAGACGCCGCCGCTTCGCCCAAATCCTTATGCCGGTTCGACGACTGGACAAAATACTGTTTATCTTCCCAGCGCAGGCAGGTTAATGTACCACCCCAGCAGCAGCCGGTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTCAGTGGGGCAGGGGCCTCTTCCGGCGATTCTTACTAGCTAGTATGCATAGGTGCTGAAATATAAAGTTTGTGTTTCTAAAACACACGTGGTACGTACGATAACGTACAGTGTTTTTCCCTCCACTTAAATCGAAGGGTAGTGTCTTGGAGCGCGCGGAGTAAACATATATGGTTCATATATGTCCGTAGGCACGTAAAAAAAGCGAGGGATTCGAATTCCCCCGGAACCCCCGGTTGGGGCCCACGCCTCGATCGAGCAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAGCTTTCCCGCGGCCAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCACTCACTGACTCGCTGCGCTCGGTCGCTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGATCCACGCTCACCGGCTCCAGATTTATCAGCAATA…
encoding: naseq.html

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245_backbone_seq/1
type: Sequence
persistent identity: pEPQD0KN0245 backbone seq
display id: pEPQD0KN0245_backbone_seq
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
elements: GCTTCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGCCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCTTCGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGATCCCGCGAAATTAATACGACTCACTATAGGGACACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATAACCAT
encoding: naseq.html

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283_seq/1
type: Sequence
persistent identity: pEPQD0KN0283 seq
display id: pEPQD0KN0283_seq
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
elements: AAACGCCAGCAACGCGATCCCGCGACGAATAAGCCAGATGCTACACAATTAGGCTTGTACATACTGTCGTTAGAACGCGGCTACAATTAATACATAACCTTATGTATCATACACATACGATTTAGGTGACACTATAGAACTCACCTATCTCCCCAACACCTAATAACATTCAATCACTCTTTCCACTAACCACCTATCTACATCACCAAGATATCACTAGTTCTCGAGCTCCATAGAGACCGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTAGGTCTCAGCTTCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGCCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCTTCGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGG…
encoding: naseq.html

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0024_seq/1
type: Sequence
persistent identity: pEPQD0KN0024 seq
display id: pEPQD0KN0024_seq
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
elements: AACGCCAGCAACGCGATCCCGCGAAATTAATACGACTCACTATAGGGACACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGAGAAAAAAATAATGTGAGACCGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTAGGTCTCAGCTTCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGCCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCTTCGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCT…
encoding: naseq.html

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0246_seq/1
type: Sequence
persistent identity: pEPQD0CB0246 seq
display id: pEPQD0CB0246_seq
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
elements: GGCGTAGAGGATCTGGCTAGCGATGACCCTGCTGATTGGTTCGCTGACCATTTCCGGGTGCGGGACGGCGTTACCAGAAACTCAGAAGGTTCGTCCAACCAAACCGACTCTGGCGGCAGTTTACGAGAGAGATGATAGGGTCTGCTTCAGTAAGCCAGATGCTACACAATTAGGCTTGTACATACTGTCGTTAGAACGCGGCTACAATTAATACATAACCTTATGTATCATACACATACGATTTAGGTGACACTATAGAACTCACCTATCTCCCCAACACCTAATAACATTCAATCACTCTTTCCACTAACCACCTATCTACATCACCAAGATATCACTAGTTCTCGAGCTCCATTGAGACCGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTAGGTCTCAGCTTCGGGTTATAATTACCTCAGGTCGACGTCCCATGGTTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCTATATCAGGCGGTGTTTAAGACGCCGCCGCTTCGCCCAAATCCTTATGCCGGTTCGACGACTGGACAAAATACTGTTTATCTTCCCAGCGCAGGCAGGTTAATGTACCACCCCAGCAGCAGCCGGTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTCAGTGGGGCAGGGGCCTCTTCCGGCGATTCTTACTAGCTAGTATGCATAGGTGCTGAAATATAAAGTTTGTGTTTCTAAAACACACGTGGTACGTACGATAACGTACAGTGTTTTTCCCTCCACTTAAATCGAAGGGTAGTGTCTTGGAGCGCGCGGAGTAAACATATATGGTTCATATATGTCCGTAGGCACGTAAAAAAAGCGAGGGATTCGAATTCCCCCGGAACCCCCGGTTGGGGCCCACGCCTCGATCGAGCAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAGCTTTCCCGCGGCCAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCACTCACTGACTCGCTGCGCTCGGTCGCTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTT…
encoding: naseq.html

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284_backbone_seq/1
type: Sequence
persistent identity: pEPQD0KN0284 backbone seq
display id: pEPQD0KN0284_backbone_seq
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
elements: GCTTCGGGTTATAATTACCTCAGGTCGACGTCCCATGGTTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCTATATCAGGCGGTGTTTAAGACGCCGCCGCTTCGCCCAAATCCTTATGCCGGTTCGACGACTGGACAAAATACTGTTTATCTTCCCAGCGCAGGCAGGTTAATGTACCACCCCAGCAGCAGCCGGTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTCAGTGGGGCAGGGGCCTCTTCCGGCGATTCTTACTAGCTAGTATGCATAGGTGCTGAAATATAAAGTTTGTGTTTCTAAAACACACGTGGTACGTACGATAACGTACAGTGTTTTTCCCTCCACTTAAATCGAAGGGTAGTGTCTTGGAGCGCGCGGAGTAAACATATATGGTTCATATATGTCCGTAGGCACGTAAAAAAAGCGAGGGATTCGAATTCCCCCGGAACCCCCGGTTGGGGCCCACGCCTCGATCGAGCAAAAAAAAAAAAAGAAAAAAAAAAAAAAAAAGCTTTCCCGCGGCCAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGCCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCTTCGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGAT…
encoding: naseq.html

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285_seq/1
type: Sequence
persistent identity: pEPQD0KN0285 seq
display id: pEPQD0KN0285_seq
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
elements: ATCCATGTTGGAATTTAATCGCGGCTTCGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGATCCCGCGACGAATAAGCCAGATGCTACACAATTAGGCTTGTACATACTGTCGTTAGAACGCGGCTACAATTAATACATAACCTTATGTATCATACACATACGATTTAGGTGACACTATAGAACTCACCTATCTCCCCAACACCTAATAACATTCAATCACTCTTTCCACTAACCACCTATCTACATCACCAAGATATCACTAGTTCTCGAGCTCCATAGAGACCGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTAGGTCTCAGCTTCGGGTTATAATTACCTCAGGTCGACGTCCCATGGTTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCTATATCAGGCGGTGTTTAAGACGCCGCCGCTTCGCCCAAATCCTTATGCCGGTTCGACGACTGGACAAAATACTGTTTATCTTCCCAGCGCAGGCAGGTTAATGTACCACCCCAGCAGCAGCCGGTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTCAGTGGGGCAGGGGCCTCTTCCGGCGATTCTTACTAGCTAGTATGCATAGGTGCTGAAATATAAAGTTT…
encoding: naseq.html

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0285_backbone_seq/1
type: Sequence
persistent identity: pEPQD0KN0285 backbone seq
display id: pEPQD0KN0285_backbone_seq
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
elements: GCTTCGGGTTATAATTACCTCAGGTCGACGTCCCATGGTTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCTATATCAGGCGGTGTTTAAGACGCCGCCGCTTCGCCCAAATCCTTATGCCGGTTCGACGACTGGACAAAATACTGTTTATCTTCCCAGCGCAGGCAGGTTAATGTACCACCCCAGCAGCAGCCGGTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTCAGTGGGGCAGGGGCCTCTTCCGGCGATTCTTACTAGCTAGTATGCATAGGTGCTGAAATATAAAGTTTGTGTTTCTAAAACACACGTGGTACGTACGATAACGTACAGTGTTTTTCCCTCCACTTAAATCGAAGGGTAGTGTCTTGGAGCGCGCGGAGTAAACATATATGGTTCATATATGTCCGTAGGCACGTAAAAAAAGCGAGGGATTCGAATTCCCCCGGAACCCCCGGTTGGGGCCCACGCCTCGATCGAGCAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAGCTTTCCCGCGGCCAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGCCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCTTCGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGAC…
encoding: naseq.html

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244_seq/1
type: Sequence
persistent identity: pEPQD0KN0244 seq
display id: pEPQD0KN0244_seq
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
elements: AACGCCAGCAACGCGATCCCGCGAAATTAATACGACTCACTATAGGGACACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGAGAAAAAAATATCCATTGAGACCGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTAGGTCTCAGCTTCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGCCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCTTCGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTG…
encoding: naseq.html

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0244_backbone_seq/1
type: Sequence
persistent identity: pEPQD0KN0244 backbone seq
display id: pEPQD0KN0244_backbone_seq
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
elements: GCTTCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGCCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCTTCGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGATCCCGCGAAATTAATACGACTCACTATAGGGACACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGAGAAAAAAATATCCAT
encoding: naseq.html

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0245_seq/1
type: Sequence
persistent identity: pEPQD0KN0245 seq
display id: pEPQD0KN0245_seq
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
elements: ACGCCAGCAACGCGATCCCGCGAAATTAATACGACTCACTATAGGGACACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATAACCATTGAGACCGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTAGGTCTCAGCTTCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGCCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCTTCGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGG…
encoding: naseq.html

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0283_backbone_seq/1
type: Sequence
persistent identity: pEPQD0KN0283 backbone seq
display id: pEPQD0KN0283_backbone_seq
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
elements: GCTTCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGCCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCTTCGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGATCCCGCGACGAATAAGCCAGATGCTACACAATTAGGCTTGTACATACTGTCGTTAGAACGCGGCTACAATTAATACATAACCTTATGTATCATACACATACGATTTAGGTGACACTATAGAACTCACCTATCTCCCCAACACCTAATAACATTCAATCACTCTTTCCACTAACCACCTATCTACATCACCAAGATATCACTAGTTCTCGAGCTCCAT
encoding: naseq.html

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0KN0284_seq/1
type: Sequence
persistent identity: pEPQD0KN0284 seq
display id: pEPQD0KN0284_seq
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
elements: AAACGCCAGCAACGCGATCCCGCGACGAATAAGCCAGATGCTACACAATTAGGCTTGTACATACTGTCGTTAGAACGCGGCTACAATTAATACATAACCTTATGTATCATACACATACGATTTAGGTGACACTATAGAACTCACCTATCTCCCCAACACCTAATAACATTCAATCACTCTTTCCACTAACCACCTATCTACATCACCAAGATATCACTAGTTCTCGAGCTAATGAGAGACCGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTAGGTCTCAGCTTCGGGTTATAATTACCTCAGGTCGACGTCCCATGGTTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCTATATCAGGCGGTGTTTAAGACGCCGCCGCTTCGCCCAAATCCTTATGCCGGTTCGACGACTGGACAAAATACTGTTTATCTTCCCAGCGCAGGCAGGTTAATGTACCACCCCAGCAGCAGCCGGTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTCAGTGGGGCAGGGGCCTCTTCCGGCGATTCTTACTAGCTAGTATGCATAGGTGCTGAAATATAAAGTTTGTGTTTCTAAAACACACGTGGTACGTACGATAACGTACAGTGTTTTTCCCTCCACTTAAATCGAAGGGTAGTGTCTTGGAGCGCGCGGAGTAAACATATATGGTTCATATATGTCCGTAGGCACGTAAAAAAAGCGAGGGATTCGAATTCCCCCGGAACCCCCGGTTGGGGCCCACGCCTCGATCGAGCAAAAAAAAAAAAAGAAAAAAAAAAAAAAAAAGCTTTCCCGCGGCCAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGCCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGG…
encoding: naseq.html

http://hub.openbioeconomy.org/user/ct677/CellFreeExpression/pEPQD0CB0026_seq/1
type: Sequence
persistent identity: pEPQD0CB0026 seq
display id: pEPQD0CB0026_seq
version: 1
OBI_0001617: 34693026
owned by: ct677
owned by: fxbuson
owned by: ykh26
elements: GGCGTAGAGGATCTGGCTAGCGATGACCCTGCTGATTGGTTCGCTGACCATTTCCGGGTGCGGGACGGCGTTACCAGAAACTCAGAAGGTTCGTCCAACCAAACCGACTCTGGCGGCAGTTTACGAGAGAGATGATAGGGTCTGCTTCAGTAAGCCAGATGCTACACAATTAGGCTTGTACATACTGTCGTTAGAACGCGGCTACAATTAATACATAACCTTATGTATCATACACATACGATTTAGGTGACACTATAGAACTCACCTATCTCCCCAACACCTAATAACATTCAATCACTCTTTCCACTAACCACCTATCTACATCACCAAGATATCACTAGTTCTCGAGCTAATGTGAGACCGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTAGGTCTCAGCTTCGGGTTATAATTACCTCAGGTCGACGTCCCATGGTTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCTATATCAGGCGGTGTTTAAGACGCCGCCGCTTCGCCCAAATCCTTATGCCGGTTCGACGACTGGACAAAATACTGTTTATCTTCCCAGCGCAGGCAGGTTAATGTACCACCCCAGCAGCAGCCGGTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTCAGTGGGGCAGGGGCCTCTTCCGGCGATTCTTACTAGCTAGTATGCATAGGTGCTGAAATATAAAGTTTGTGTTTCTAAAACACACGTGGTACGTACGATAACGTACAGTGTTTTTCCCTCCACTTAAATCGAAGGGTAGTGTCTTGGAGCGCGCGGAGTAAACATATATGGTTCATATATGTCCGTAGGCACGTAAAAAAAGCGAGGGATTCGAATTCCCCCGGAACCCCCGGTTGGGGCCCACGCCTCGATCGAGCAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAGCTTTCCCGCGGCCAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCACTCACTGACTCGCTGCGCTCGGTCGCTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTT…
encoding: naseq.html