LOCUS pEPQD0CB0246 4281 bp DNA circular 23-Jun-2025 ACCESSION pEPQD0CB0246 VERSION pEPQD0CB0246.1 FEATURES Location/Qualifiers CDS complement(452..775) /OBI_0001617=34693026 /label={'lacZ-alpha', 'lacZ beta-galactosidase alpha peptide'} promoter complement(1619..1742) /OBI_0001617=34693026 /label=Plac promoter promoter complement(782..905) /OBI_0001617=34693026 /label=Plac promoter misc_feature 702..758 /OBI_0001617=34693026 /label=MCS misc_feature 795..811 /OBI_0001617=34693026 /label=lac operator misc_feature 1632..1648 /OBI_0001617=34693026 /label=lac operator promoter complement(1656..1686) /OBI_0001617=34693026 /label=lac promoter promoter complement(819..849) /OBI_0001617=34693026 /label=lac promoter misc_feature 864..885 /OBI_0001617=34693026 /label=CAP binding site misc_feature 1701..1722 /OBI_0001617=34693026 /label=CAP binding site rep_origin complement(2010..2598) /OBI_0001617=34693026 /label=pUC origin of replication (derivative of pBR322/pMB1/ColE1) misc_feature 259..330 /OBI_0001617=34693026 /label=E01 Translational Enhancer misc_feature complement(1062..1313) /OBI_0001617=34693026 /label=diadenosine tetraphosphatase (apaH) C-terminal fragment ( aa 198-280) AVI54590.1 from E coli MG1655 terminator 985..1061 /OBI_0001617=34693026 /label=folA/apaH terminator RBS complement(3195..3201) /OBI_0001617=34693026 /label=RBS promoter complement(1657..1686) /OBI_0001617=34693026 /label=lac_promoter promoter complement(820..849) /OBI_0001617=34693026 /label=lac_promoter CDS complement(2769..3629) /OBI_0001617=34693026 /label=beta-lactamase, ampicillin resistance (ampR) promoter complement(3671..3699) /OBI_0001617=34693026 /label=AmpR_promoter misc_feature complement(4030..4281) /OBI_0001617=34693026 /label={'tet (887 - 636)', 'tet'} rep_origin complement(1995..2614) /OBI_0001617=34693026 /label=pBR322 (from pUC18) promoter complement(3630..3734) /OBI_0001617=34693026 /label=AmpR promoter ORIGIN 1 GGCGTAGAGG ATCTGGCTAG CGATGACCCT GCTGATTGGT TCGCTGACCA TTTCCGGGTG 61 CGGGACGGCG TTACCAGAAA CTCAGAAGGT TCGTCCAACC AAACCGACTC TGGCGGCAGT 121 TTACGAGAGA GATGATAGGG TCTGCTTCAG TAAGCCAGAT GCTACACAAT TAGGCTTGTA 181 CATACTGTCG TTAGAACGCG GCTACAATTA ATACATAACC TTATGTATCA TACACATACG 241 ATTTAGGTGA CACTATAGAA CTCACCTATC TCCCCAACAC CTAATAACAT TCAATCACTC 301 TTTCCACTAA CCACCTATCT ACATCACCAA GATATCACTA GTTCTCGAGC TCCATTGAGA 361 CCGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG 421 CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA 481 GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA 541 GGCGCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT 601 CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC 661 CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAATTCGAG CTCGGTACCC 721 GGGGATCCTC TAGAGTCGAC CTGCAGGCAT GCAAGCTTGG CGTAATCATG GTCATAGCTG 781 TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA 841 AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA 901 CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTAG GTCTCAGCTT CGGGTTATAA 961 TTACCTCAGG TCGACGTCCC ATGGTTTTGT ATAGAATTTA CGGCTAGCGC CGGATGCGAC 1021 GCCGGTCGCG TCTTATCCGG CCTTCCTATA TCAGGCGGTG TTTAAGACGC CGCCGCTTCG 1081 CCCAAATCCT TATGCCGGTT CGACGACTGG ACAAAATACT GTTTATCTTC CCAGCGCAGG 1141 CAGGTTAATG TACCACCCCA GCAGCAGCCG GTATCCAGCG CGTATATACC TTCCGGCGTA 1201 CCTTTGCCCT CCAGCGATGC CCAGTGACCA AAGGCGATGC TGTATTCTTC AGCGACAGGG 1261 CCAGGAATCG CAAACCACGG TTTCAGTGGG GCAGGGGCCT CTTCCGGCGA TTCTTACTAG 1321 CTAGTATGCA TAGGTGCTGA AATATAAAGT TTGTGTTTCT AAAACACACG TGGTACGTAC 1381 GATAACGTAC AGTGTTTTTC CCTCCACTTA AATCGAAGGG TAGTGTCTTG GAGCGCGCGG 1441 AGTAAACATA TATGGTTCAT ATATGTCCGT AGGCACGTAA AAAAAGCGAG GGATTCGAAT 1501 TCCCCCGGAA CCCCCGGTTG GGGCCCACGC CTCGATCGAG CAAAAAAAAA AAAAAAGAAA 1561 AAAAAAAAAA AAAAAAGCTT TCCCGCGGCC AGCTTGGCGT AATCATGGTC ATAGCTGTTT 1621 CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG 1681 TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG 1741 CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG 1801 GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CACTCACTGA CTCGCTGCGC 1861 TCGGTCGCTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC 1921 ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG 1981 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT 2041 CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG 2101 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA 2161 TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG 2221 TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT 2281 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC 2341 GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC 2401 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT 2461 GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC 2521 GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC 2581 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG 2641 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG 2701 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG 2761 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT 2821 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA 2881 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GATCCACGCT CACCGGCTCC AGATTTATCA 2941 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC 3001 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT 3061 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG 3121 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC 3181 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG 3241 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA 3301 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGCG TATGCGGCGA 3361 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA 3421 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG 3481 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT 3541 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA 3601 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT 3661 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 3721 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT 3781 ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT CGCGCGTTTC 3841 GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG 3901 TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT 3961 CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATTCGACG 4021 CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG 4081 CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC 4141 GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG 4201 ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT 4261 GATGCCGGCC ACGATGCGTC C //