| Types | circular DnaRegion |
| Roles | engineered_plasmid |
| Sequences | OP_054_seq (Version 1) |
| Access | Instance | Definition |
| public public public public public public | TEST_003_0_comp TEST_004_0_comp TEST_005_0_comp TEST_019_0_comp TEST_022_0_comp TEST_092_0_comp | high-copy-number ColE1/pMB1/pBR322/pUC origin of replication cat / CmR lambda t0 terminator nosT (MoClo) 3TERM_35S (MoClo) 3TERM_Nos-35S |
| Sequence Annotation | Location | Component / Role(s) |
| BsaI recognition site BsaI recognition site TEST_003_0_annot TEST_004_0_annot TEST_005_0_annot TEST_019_0_annot TEST_022_0_annot TEST_092_0_annot | 236,241 complement(725,730) complement(989,1577) complement(1875,2534) complement(1760,1854) 247,500 516,719 247,719 | restriction_enzyme_recognition_site restriction_enzyme_recognition_site high-copy-number ColE1/pMB1/pBR322/pUC origin of replication cat / CmR lambda t0 terminator nosT (MoClo) 3TERM_35S (MoClo) 3TERM_Nos-35S |