Open DNA Collections Hub

Sequence Annotation	Location	Component / Role(s)
SapI recognition site SapI recognition site TEST_006_0_annot TEST_007_0_annot TEST_008_0_annot TEST_009_0_annot TEST_010_0_annot STA region from pVS1 plasmid A.tumefaciens plasmid left boundary A.tumefaciens plasmid right boundary TEST_014_0_annot TEST_019_0_annot TEST_020_0_annot TEST_042_0_annot part of AT4G19150 TEST_046_0_annot S4xAla linker intron in 5UTR_MpNOP1 exon 5UTR_MpNOP1 exon in 5UTR_MpNOP1 TEST_064_0_annot TEST_065_0_annot TEST_073_0_annot TEST_091_0_annot	468,474 complement(7630,7636) 11702,11842 12028,12616 8967,9596 10025,11098 11164,11358 8728,9728 433,458 7642,7666 complement(12704,13519) 7148,7401 7417,7620 6174,6869 6900,7127 6888,7127 6873,6887 3848,6154 3446,3847 6155,6172 484,3441 3446,6172 complement(12013,12627) 7148,7620	restriction_enzyme_recognition_site restriction_enzyme_recognition_site basis of mobility region from pBR322 high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 pVS1 StaA stability protein pVS1 RepA replication protein pVS1 oriV origin of replication engineered_region engineered_region engineered_region nptI / KanR nosT (MoClo) 35ST (MoClo) mScarlet-I no Stop engineered_region N7-NLS junction engineered_region engineered_region engineered_region PROM_MpNOP1 Tak 5UTR_MpNOP1 Tak rep (pMB1) 3TERM_Nos-35S

Access	Instance	Definition
public public public public public public public public public public public public public public	TEST_006_0_comp TEST_007_0_comp TEST_008_0_comp TEST_009_0_comp TEST_010_0_comp TEST_014_0_comp TEST_019_0_comp TEST_020_0_comp TEST_042_0_comp TEST_046_0_comp TEST_064_0_comp TEST_065_0_comp TEST_073_0_comp TEST_091_0_comp	basis of mobility region from pBR322 high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 pVS1 StaA stability protein pVS1 RepA replication protein pVS1 oriV origin of replication nptI / KanR nosT (MoClo) 35ST (MoClo) mScarlet-I no Stop N7-NLS PROM_MpNOP1 Tak 5UTR_MpNOP1 Tak rep (pMB1) 3TERM_Nos-35S

synbiohub#ownedBy	user/ykh26
synbiohub#topLevel	OP_064/1

L1_NOP1:mS-N7-Ck2