Open DNA Collections Hub

Sequence Annotation	Location	Component / Role(s)
SapI recognition site SapI recognition site TEST_006_0_annot TEST_007_0_annot TEST_008_0_annot TEST_009_0_annot TEST_010_0_annot STA region from pVS1 plasmid A.tumefaciens plasmid left boundary A.tumefaciens plasmid right boundary TEST_014_0_annot TEST_019_0_annot TEST_020_0_annot exon 5UTR_MpUBE2 Mapoly0106s0019 TEST_024_0_annot intron in 5UTR_MpUBE2 TEST_026_0_annot exon 5UTR_MpUBE2 Mapoly0106s0019 TEST_043_0_annot part of AT4G19150 TEST_046_0_annot S4xAla linker TEST_073_0_annot TEST_091_0_annot TEST_097_0_annot	468,474 complement(4984,4990) 9056,9196 9382,9970 6321,6950 7379,8452 8518,8712 6082,7082 433,458 4996,5020 complement(10058,10873) 4502,4755 4771,4974 2280,3051 2280,3505 3052,3272 484,2275 3273,3505 3507,4223 4254,4481 4242,4481 4227,4241 complement(9367,9981) 4502,4974 3510,4223	restriction_enzyme_recognition_site restriction_enzyme_recognition_site basis of mobility region from pBR322 high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 pVS1 StaA stability protein pVS1 RepA replication protein pVS1 oriV origin of replication engineered_region engineered_region engineered_region nptI / KanR nosT (MoClo) 35ST (MoClo) engineered_region 5UTR_MpUBE2 Mapoly0106s0019 engineered_region PROM_MpUBE2 Mapoly0106s0019 engineered_region mTurquoise2 no Stop engineered_region N7-NLS junction rep (pMB1) 3TERM_Nos-35S mTurquoise2 no ATG no STOP

Access	Instance	Definition
public public public public public public public public public public public public public public public	TEST_006_0_comp TEST_007_0_comp TEST_008_0_comp TEST_009_0_comp TEST_010_0_comp TEST_014_0_comp TEST_019_0_comp TEST_020_0_comp TEST_024_0_comp TEST_026_0_comp TEST_043_0_comp TEST_046_0_comp TEST_073_0_comp TEST_091_0_comp TEST_097_0_comp	basis of mobility region from pBR322 high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 pVS1 StaA stability protein pVS1 RepA replication protein pVS1 oriV origin of replication nptI / KanR nosT (MoClo) 35ST (MoClo) 5UTR_MpUBE2 Mapoly0106s0019 PROM_MpUBE2 Mapoly0106s0019 mTurquoise2 no Stop N7-NLS rep (pMB1) 3TERM_Nos-35S mTurquoise2 no ATG no STOP

synbiohub#ownedBy	user/ykh26
synbiohub#topLevel	OP_065/1

L1_UBE2:mT-N7-Ck2