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L2_UBE2:mT-N7-CsA

OP_071 Version 1

Component

Details

Types	circular DnaRegion
Roles	engineered_plasmid
Sequences	OP_071_seq (Version 1)

Components

Access	Instance	Definition
public public public public public public public public public public public public public public public public public public public	TEST_006_0_comp TEST_007_0_comp TEST_008_0_comp TEST_009_0_comp TEST_010_0_comp TEST_018_0_comp TEST_019_0_comp TEST_020_0_comp TEST_022_0_comp TEST_024_0_comp TEST_026_0_comp TEST_029_0_comp TEST_032_0_comp TEST_040_0_comp TEST_043_0_comp TEST_046_0_comp TEST_073_0_comp TEST_091_0_comp TEST_097_0_comp	basis of mobility region from pBR322 high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 pVS1 StaA stability protein pVS1 RepA replication protein pVS1 oriV origin of replication aadA-1 / Sm resistance nosT (MoClo) 35ST (MoClo) 3TERM_35S (MoClo) 5UTR_MpUBE2 Mapoly0106s0019 PROM_MpUBE2 Mapoly0106s0019 min 35S pro hptII / HygR p5-35S mTurquoise2 no Stop N7-NLS rep (pMB1) 3TERM_Nos-35S mTurquoise2 no ATG no STOP

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BsaI recognition site BsaI recognition site TEST_006_0_annot TEST_007_0_annot TEST_008_0_annot TEST_009_0_annot TEST_010_0_annot STA region from pVS1 plasmid A.tumefaciens plasmid left boundary A.tumefaciens plasmid right boundary TEST_018_0_annot TEST_019_0_annot TEST_020_0_annot Odd-spacer Odd-spacer TEST_022_0_annot exon 5UTR_MpUBE2 Mapoly0106s0019 TEST_024_0_annot intron in 5UTR_MpUBE2 TEST_026_0_annot exon 5UTR_MpUBE2 Mapoly0106s0019 TATA box TEST_029_0_annot TEST_032_0_annot TEST_040_0_annot TEST_043_0_annot part of AT4G19150 TEST_046_0_annot S4xAla linker TEST_073_0_annot TEST_091_0_annot TEST_097_0_annot	469,474 complement(7530,7535) 11602,11742 11928,12516 8867,9496 9925,10998 11064,11258 8628,9628 433,458 7542,7566 complement(12604,13614) 6830,7083 7099,7302 2599,2798 7316,7515 2382,2585 4608,5379 4608,5833 5380,5600 2812,4603 5601,5833 1288,1294 1272,1317 1352,2377 489,1323 5835,6551 6582,6809 6570,6809 6555,6569 complement(11913,12527) 6830,7302 5838,6551	restriction_enzyme_recognition_site restriction_enzyme_recognition_site basis of mobility region from pBR322 high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 pVS1 StaA stability protein pVS1 RepA replication protein pVS1 oriV origin of replication engineered_region engineered_region engineered_region aadA-1 / Sm resistance nosT (MoClo) 35ST (MoClo) engineered_region engineered_region 3TERM_35S (MoClo) engineered_region 5UTR_MpUBE2 Mapoly0106s0019 engineered_region PROM_MpUBE2 Mapoly0106s0019 engineered_region engineered_region min 35S pro hptII / HygR p5-35S mTurquoise2 no Stop engineered_region N7-NLS junction rep (pMB1) 3TERM_Nos-35S mTurquoise2 no ATG no STOP

Other Properties

synbiohub#ownedBy	user/ykh26
synbiohub#topLevel	OP_071/1

Member of these Collections

Marchantia_OpenPlant_Toolkit

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