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L2_UBE2:eG-Lt-CsA

OP_072 Version 1

Component

Details

Types	circular DnaRegion
Roles	engineered_plasmid
Sequences	OP_072_seq (Version 1)

Components

Access	Instance	Definition
public public public public public public public public public public public public public public public public public public public public	TEST_006_0_comp TEST_007_0_comp TEST_008_0_comp TEST_009_0_comp TEST_010_0_comp TEST_018_0_comp TEST_019_0_comp TEST_020_0_comp TEST_022_0_comp TEST_024_0_comp TEST_026_0_comp TEST_029_0_comp TEST_032_0_comp TEST_033_0_comp TEST_039_0_comp TEST_040_0_comp TEST_041_0_comp TEST_055_0_comp TEST_073_0_comp TEST_091_0_comp	basis of mobility region from pBR322 high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 pVS1 StaA stability protein pVS1 RepA replication protein pVS1 oriV origin of replication aadA-1 / Sm resistance nosT (MoClo) 35ST (MoClo) 3TERM_35S (MoClo) 5UTR_MpUBE2 Mapoly0106s0019 PROM_MpUBE2 Mapoly0106s0019 min 35S pro hptII / HygR AtRCI2B AT3G05890 Lti6b p5-35S eGFP no Stop eGFP no ATG no Stop rep (pMB1) 3TERM_Nos-35S

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BsaI recognition site BsaI recognition site TEST_006_0_annot TEST_007_0_annot TEST_008_0_annot TEST_009_0_annot TEST_010_0_annot STA region from pVS1 plasmid A.tumefaciens plasmid left boundary A.tumefaciens plasmid right boundary TEST_018_0_annot TEST_019_0_annot TEST_020_0_annot Odd-spacer Odd-spacer TEST_022_0_annot exon 5UTR_MpUBE2 Mapoly0106s0019 TEST_024_0_annot intron in 5UTR_MpUBE2 TEST_026_0_annot exon 5UTR_MpUBE2 Mapoly0106s0019 TATA box TEST_029_0_annot TEST_032_0_annot TEST_033_0_annot SG10xAla linker TEST_039_0_annot TEST_040_0_annot TEST_041_0_annot TEST_055_0_annot TEST_073_0_annot TEST_091_0_annot CTAG_linker-Lti6b	469,474 complement(7497,7502) 11569,11709 11895,12483 8834,9463 9892,10965 11031,11225 8595,9595 433,458 7509,7533 complement(12571,13581) 7010,7263 7279,7482 2599,2798 2812,3011 2382,2585 4821,5592 4821,6046 5593,5813 3025,4816 5814,6046 1288,1294 1272,1317 1352,2377 6822,6986 6768,6803 6804,6986 489,1323 6048,6764 6051,6764 complement(11880,12494) 7010,7482 6771,6989	restriction_enzyme_recognition_site restriction_enzyme_recognition_site basis of mobility region from pBR322 high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 pVS1 StaA stability protein pVS1 RepA replication protein pVS1 oriV origin of replication engineered_region engineered_region engineered_region aadA-1 / Sm resistance nosT (MoClo) 35ST (MoClo) engineered_region engineered_region 3TERM_35S (MoClo) engineered_region 5UTR_MpUBE2 Mapoly0106s0019 engineered_region PROM_MpUBE2 Mapoly0106s0019 engineered_region engineered_region min 35S pro hptII / HygR AtRCI2B AT3G05890 junction Lti6b p5-35S eGFP no Stop eGFP no ATG no Stop rep (pMB1) 3TERM_Nos-35S junction

Other Properties

synbiohub#ownedBy	user/ykh26
synbiohub#topLevel	OP_072/1

Member of these Collections

Marchantia_OpenPlant_Toolkit

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