Open DNA Collections Hub

Sequence Annotation	Location	Component / Role(s)
SapI recognition site SapI recognition site TEST_006_0_annot TEST_007_0_annot TEST_008_0_annot TEST_009_0_annot TEST_010_0_annot STA region from pVS1 plasmid A.tumefaciens plasmid left boundary A.tumefaciens plasmid right boundary TEST_014_0_annot TEST_019_0_annot TEST_020_0_annot TATA box TEST_034_0_annot NLS_SV40 Intron in 5UTR_MpEF1a exon 5UTR_MpEF1a TEST_045_0_annot 5UTR_MpEF1a exon 5UTR_MpEF1a TEST_073_0_annot TEST_088_0_annot TEST_091_0_annot	468,474 complement(6861,6867) 10933,11073 11259,11847 8198,8827 9256,10329 10395,10589 7959,8959 433,458 6873,6897 complement(11935,12750) 6379,6632 6648,6851 1439,1445 484,1485 6335,6355 1845,2204 2205,2217 2219,6358 1486,2217 1486,1844 complement(11244,11858) 2219,6355 6379,6851	restriction_enzyme_recognition_site restriction_enzyme_recognition_site basis of mobility region from pBR322 high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 pVS1 StaA stability protein pVS1 RepA replication protein pVS1 oriV origin of replication engineered_region engineered_region engineered_region nptI / KanR nosT (MoClo) 35ST (MoClo) engineered_region PROM_MpEF1a engineered_region engineered_region engineered_region AtcoCas9 (Puchta)STOP engineered_region engineered_region rep (pMB1) AtcoCas9 (Puctha) NoStop 3TERM_Nos-35S

Access	Instance	Definition
public public public public public public public public public public public public public	TEST_006_0_comp TEST_007_0_comp TEST_008_0_comp TEST_009_0_comp TEST_010_0_comp TEST_014_0_comp TEST_019_0_comp TEST_020_0_comp TEST_034_0_comp TEST_045_0_comp TEST_073_0_comp TEST_088_0_comp TEST_091_0_comp	basis of mobility region from pBR322 high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 pVS1 StaA stability protein pVS1 RepA replication protein pVS1 oriV origin of replication nptI / KanR nosT (MoClo) 35ST (MoClo) PROM_MpEF1a AtcoCas9 (Puchta)STOP rep (pMB1) AtcoCas9 (Puctha) NoStop 3TERM_Nos-35S

synbiohub#ownedBy	user/ykh26
synbiohub#topLevel	OP_073/1

L1_Cas9-Ck4_