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L2_lacZgRNA-Cas9-CsA

OP_076 Version 1

Component

Details

Types	circular DnaRegion
Roles	engineered_plasmid
Sequences	OP_076_seq (Version 1)

Components

Access	Instance	Definition
public public public public public public public public public public public public public public public public public public public public public public	TEST_006_0_comp TEST_007_0_comp TEST_008_0_comp TEST_009_0_comp TEST_010_0_comp TEST_015_0_comp TEST_016_0_comp TEST_017_0_comp TEST_018_0_comp TEST_019_0_comp TEST_020_0_comp TEST_022_0_comp TEST_029_0_comp TEST_031_0_comp TEST_032_0_comp TEST_034_0_comp TEST_045_0_comp TEST_066_0_comp TEST_068_0_comp TEST_073_0_comp TEST_088_0_comp TEST_091_0_comp	basis of mobility region from pBR322 high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 pVS1 StaA stability protein pVS1 RepA replication protein pVS1 oriV origin of replication lac promoter lac operator LacZa aadA-1 / Sm resistance nosT (MoClo) 35ST (MoClo) 3TERM_35S (MoClo) min 35S pro p5-MpU6 Sugano hptII / HygR PROM_MpEF1a AtcoCas9 (Puchta)STOP p5-35Sx2 p5-35Sx2 rep (pMB1) AtcoCas9 (Puctha) NoStop 3TERM_Nos-35S

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BsaI recognition site BsaI recognition site SapI recognition site SapI recognition site TEST_006_0_annot TEST_007_0_annot TEST_008_0_annot TEST_009_0_annot TEST_010_0_annot STA region from pVS1 plasmid A.tumefaciens plasmid left boundary A.tumefaciens plasmid right boundary TEST_015_0_annot TEST_016_0_annot TEST_017_0_annot TEST_018_0_annot TEST_019_0_annot TEST_020_0_annot Odd-spacer TEST_022_0_annot TATA box TATA box TEST_029_0_annot 35S 35S TEST_031_0_annot TEST_032_0_annot TEST_034_0_annot NLS_SV40 Intron in 5UTR_MpEF1a exon 5UTR_MpEF1a TEST_045_0_annot 5UTR_MpEF1a exon 5UTR_MpEF1a TEST_066_0_annot TEST_068_0_annot TEST_073_0_annot TEST_088_0_annot TEST_091_0_annot	469,474 complement(11899,11904) 5385,5391 complement(4801,4807) 15971,16111 16297,16885 13236,13865 14294,15367 15433,15627 12997,13997 433,458 11911,11935 complement(5264,5293) complement(5237,5257) complement(4898,5219) complement(16973,17983) 11412,11665 11681,11884 2575,2774 2358,2561 1216,1222 6472,6478 1200,1245 505,829 830,1154 2788,4799 1328,2353 5517,6518 11368,11388 6878,7237 7238,7250 7252,11391 6519,7250 6519,6877 505,1255 489,1294 complement(16282,16896) 7252,11388 11412,11884	restriction_enzyme_recognition_site restriction_enzyme_recognition_site restriction_enzyme_recognition_site restriction_enzyme_recognition_site basis of mobility region from pBR322 high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 pVS1 StaA stability protein pVS1 RepA replication protein pVS1 oriV origin of replication engineered_region engineered_region engineered_region lac promoter lac operator LacZa aadA-1 / Sm resistance nosT (MoClo) 35ST (MoClo) engineered_region 3TERM_35S (MoClo) engineered_region engineered_region min 35S pro engineered_region engineered_region p5-MpU6 Sugano hptII / HygR PROM_MpEF1a engineered_region engineered_region engineered_region AtcoCas9 (Puchta)STOP engineered_region engineered_region p5-35Sx2 p5-35Sx2 rep (pMB1) AtcoCas9 (Puctha) NoStop 3TERM_Nos-35S

Other Properties

synbiohub#ownedBy	user/ykh26
synbiohub#topLevel	OP_076/1

Member of these Collections

Marchantia_OpenPlant_Toolkit

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