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L2_2xNOP1gRNA-Cas9-CsA

OP_078 Version 1

Component

Details

Types	circular DnaRegion
Roles	engineered_plasmid
Sequences	OP_078_seq (Version 1)

Components

Access	Instance	Definition
public public public public public public public public public public public public public public public public public public public	TEST_006_0_comp TEST_007_0_comp TEST_008_0_comp TEST_009_0_comp TEST_010_0_comp TEST_018_0_comp TEST_019_0_comp TEST_020_0_comp TEST_022_0_comp TEST_029_0_comp TEST_031_0_comp TEST_031_1_comp TEST_032_0_comp TEST_034_0_comp TEST_040_0_comp TEST_045_0_comp TEST_073_0_comp TEST_088_0_comp TEST_091_0_comp	basis of mobility region from pBR322 high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 pVS1 StaA stability protein pVS1 RepA replication protein pVS1 oriV origin of replication aadA-1 / Sm resistance nosT (MoClo) 35ST (MoClo) 3TERM_35S (MoClo) min 35S pro p5-MpU6 Sugano p5-MpU6 Sugano hptII / HygR PROM_MpEF1a p5-35S AtcoCas9 (Puchta)STOP rep (pMB1) AtcoCas9 (Puctha) NoStop 3TERM_Nos-35S

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BsaI recognition site BsaI recognition site TEST_006_0_annot TEST_007_0_annot TEST_008_0_annot TEST_009_0_annot TEST_010_0_annot STA region from pVS1 plasmid A.tumefaciens plasmid left boundary A.tumefaciens plasmid right boundary TEST_018_0_annot TEST_019_0_annot TEST_020_0_annot TEST_022_0_annot TATA box TATA box TEST_029_0_annot TEST_031_0_annot TEST_031_1_annot TEST_032_0_annot TEST_034_0_annot NLS_SV40 Intron in 5UTR_MpEF1a exon 5UTR_MpEF1a TEST_040_0_annot TEST_045_0_annot 5UTR_MpEF1a exon 5UTR_MpEF1a NOP1 target_1 TEST_073_0_annot NOP1 target_2 TEST_088_0_annot TEST_091_0_annot	469,474 complement(13296,13301) 17368,17508 17694,18282 14633,15262 15691,16764 16830,17024 14394,15394 433,458 13308,13332 complement(18370,19380) 12809,13062 13078,13281 2382,2585 1288,1294 7869,7875 1272,1317 2599,4610 4757,6768 1352,2377 6914,7915 12765,12785 8275,8634 8635,8647 489,1323 8649,12788 7916,8647 7916,8274 6769,6787 complement(17679,18293) 4611,4630 8649,12785 12809,13281	restriction_enzyme_recognition_site restriction_enzyme_recognition_site basis of mobility region from pBR322 high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 pVS1 StaA stability protein pVS1 RepA replication protein pVS1 oriV origin of replication engineered_region engineered_region engineered_region aadA-1 / Sm resistance nosT (MoClo) 35ST (MoClo) 3TERM_35S (MoClo) engineered_region engineered_region min 35S pro p5-MpU6 Sugano p5-MpU6 Sugano hptII / HygR PROM_MpEF1a engineered_region engineered_region engineered_region p5-35S AtcoCas9 (Puchta)STOP engineered_region engineered_region engineered_region rep (pMB1) engineered_region AtcoCas9 (Puctha) NoStop 3TERM_Nos-35S

Other Properties

synbiohub#ownedBy	user/ykh26
synbiohub#topLevel	OP_078/1

Member of these Collections

Marchantia_OpenPlant_Toolkit

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