Open DNA Collections Hub

Sequence Annotation	Location	Component / Role(s)
SapI recognition site SapI recognition site TEST_006_0_annot TEST_007_0_annot TEST_008_0_annot TEST_009_0_annot TEST_010_0_annot STA region from pVS1 plasmid A.tumefaciens plasmid left boundary A.tumefaciens plasmid right boundary TEST_014_0_annot TEST_019_0_annot TEST_020_0_annot exon 5UTR_MpUBE2 Mapoly0106s0019 TEST_024_0_annot intron in 5UTR_MpUBE2 TEST_026_0_annot exon 5UTR_MpUBE2 Mapoly0106s0019 TEST_033_0_annot SG10xAla linker TEST_039_0_annot TEST_041_0_annot TEST_055_0_annot TEST_073_0_annot TEST_091_0_annot CTAG_linker-Lti6b	468,474 complement(4951,4957) 9023,9163 9349,9937 6288,6917 7346,8419 8485,8679 6049,7049 433,458 4963,4987 complement(10025,10840) 4469,4722 4738,4941 2280,3051 2280,3505 3052,3272 484,2275 3273,3505 4281,4445 4227,4262 4263,4445 3507,4223 3510,4223 complement(9334,9948) 4469,4941 4230,4448	restriction_enzyme_recognition_site restriction_enzyme_recognition_site basis of mobility region from pBR322 high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 pVS1 StaA stability protein pVS1 RepA replication protein pVS1 oriV origin of replication engineered_region engineered_region engineered_region nptI / KanR nosT (MoClo) 35ST (MoClo) engineered_region 5UTR_MpUBE2 Mapoly0106s0019 engineered_region PROM_MpUBE2 Mapoly0106s0019 engineered_region AtRCI2B AT3G05890 junction Lti6b eGFP no Stop eGFP no ATG no Stop rep (pMB1) 3TERM_Nos-35S junction

Access	Instance	Definition
public public public public public public public public public public public public public public public public	TEST_006_0_comp TEST_007_0_comp TEST_008_0_comp TEST_009_0_comp TEST_010_0_comp TEST_014_0_comp TEST_019_0_comp TEST_020_0_comp TEST_024_0_comp TEST_026_0_comp TEST_033_0_comp TEST_039_0_comp TEST_041_0_comp TEST_055_0_comp TEST_073_0_comp TEST_091_0_comp	basis of mobility region from pBR322 high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 pVS1 StaA stability protein pVS1 RepA replication protein pVS1 oriV origin of replication nptI / KanR nosT (MoClo) 35ST (MoClo) 5UTR_MpUBE2 Mapoly0106s0019 PROM_MpUBE2 Mapoly0106s0019 AtRCI2B AT3G05890 Lti6b eGFP no Stop eGFP no ATG no Stop rep (pMB1) 3TERM_Nos-35S

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synbiohub#topLevel	OP_067/1

L1_UBE2:eG-Lt-Ck3