Open DNA Collections Hub

Sequence Annotation	Location	Component / Role(s)
SapI recognition site SapI recognition site TEST_006_0_annot TEST_007_0_annot TEST_008_0_annot TEST_009_0_annot TEST_010_0_annot STA region from pVS1 plasmid A.tumefaciens plasmid left boundary A.tumefaciens plasmid right boundary TEST_014_0_annot TEST_019_0_annot TEST_020_0_annot exon 5UTR_MpUBE2 Mapoly0106s0019 TEST_024_0_annot intron in 5UTR_MpUBE2 TEST_026_0_annot exon 5UTR_MpUBE2 Mapoly0106s0019 TEST_033_0_annot SG10xAla linker TEST_039_0_annot TEST_042_0_annot TEST_073_0_annot TEST_091_0_annot CTAG_linker-Lti6b	468,474 complement(4930,4936) 9002,9142 9328,9916 6267,6896 7325,8398 8464,8658 6028,7028 433,458 4942,4966 complement(10004,10819) 4448,4701 4717,4920 2280,3051 2280,3505 3052,3272 484,2275 3273,3505 4260,4424 4206,4241 4242,4424 3507,4202 complement(9313,9927) 4448,4920 4209,4427	restriction_enzyme_recognition_site restriction_enzyme_recognition_site basis of mobility region from pBR322 high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 pVS1 StaA stability protein pVS1 RepA replication protein pVS1 oriV origin of replication engineered_region engineered_region engineered_region nptI / KanR nosT (MoClo) 35ST (MoClo) engineered_region 5UTR_MpUBE2 Mapoly0106s0019 engineered_region PROM_MpUBE2 Mapoly0106s0019 engineered_region AtRCI2B AT3G05890 junction Lti6b mScarlet-I no Stop rep (pMB1) 3TERM_Nos-35S junction

Access	Instance	Definition
public public public public public public public public public public public public public public public	TEST_006_0_comp TEST_007_0_comp TEST_008_0_comp TEST_009_0_comp TEST_010_0_comp TEST_014_0_comp TEST_019_0_comp TEST_020_0_comp TEST_024_0_comp TEST_026_0_comp TEST_033_0_comp TEST_039_0_comp TEST_042_0_comp TEST_073_0_comp TEST_091_0_comp	basis of mobility region from pBR322 high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 pVS1 StaA stability protein pVS1 RepA replication protein pVS1 oriV origin of replication nptI / KanR nosT (MoClo) 35ST (MoClo) 5UTR_MpUBE2 Mapoly0106s0019 PROM_MpUBE2 Mapoly0106s0019 AtRCI2B AT3G05890 Lti6b mScarlet-I no Stop rep (pMB1) 3TERM_Nos-35S

synbiohub#ownedBy	user/ykh26
synbiohub#topLevel	OP_070/1

L1_UBE2:mS-Lt-Ck4