Open DNA Collections Hub

Sequence Annotation	Location	Component / Role(s)
SapI recognition site SapI recognition site TEST_006_0_annot TEST_007_0_annot TEST_008_0_annot TEST_009_0_annot TEST_010_0_annot STA region from pVS1 plasmid A.tumefaciens plasmid left boundary A.tumefaciens plasmid right boundary TEST_014_0_annot TEST_015_0_annot TEST_016_0_annot TEST_017_0_annot TEST_031_0_annot TEST_073_0_annot	468,474 complement(3209,3215) 7281,7421 7607,8195 4546,5175 5604,6677 6743,6937 4307,5307 433,458 3221,3245 complement(8283,9098) complement(2960,2989) complement(2933,2953) complement(2594,2915) 484,2495 complement(7592,8206)	restriction_enzyme_recognition_site restriction_enzyme_recognition_site basis of mobility region from pBR322 high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 pVS1 StaA stability protein pVS1 RepA replication protein pVS1 oriV origin of replication engineered_region engineered_region engineered_region nptI / KanR lac promoter lac operator LacZa p5-MpU6 Sugano rep (pMB1)

Access	Instance	Definition
public public public public public public public public public public public	TEST_006_0_comp TEST_007_0_comp TEST_008_0_comp TEST_009_0_comp TEST_010_0_comp TEST_014_0_comp TEST_015_0_comp TEST_016_0_comp TEST_017_0_comp TEST_031_0_comp TEST_073_0_comp	basis of mobility region from pBR322 high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 pVS1 StaA stability protein pVS1 RepA replication protein pVS1 oriV origin of replication nptI / KanR lac promoter lac operator LacZa p5-MpU6 Sugano rep (pMB1)

synbiohub#ownedBy	user/ykh26
synbiohub#topLevel	OP_075/1

L1_lacZgRNA-Ck2